Sensitivity and specificity of Indirect Enzyme Linked Immuno sorbent Assay (ELISA) for diagnosis of hydatidosis in dromedary camels using hydatid cyst fluid antigens.

El-Kattan, Adel M.; Abdel-Ra’ouf, Aml M.; Yousef, Randa; AbouElnga, T. R.; Mousa, Waheed M.

doi:10.21608/jvmr.2020.69696

Cited by 3 publications

(4 citation statements)

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“…Cystic echinococcosis is a major disease in livestock and humans [40]. Its diagnosis in intermediate hosts is mainly based on necropsy procedures and in most cases the disease is asymptomatic in the early stages [41], so there is an urgency to develop alternative diagnostic methods [42], such as antibody detection through ELISA. Here, we identified and successfully deployed a potential candidate antigen, the iEg67 kDa crude antigen (from cystic fluid collected from buffalo liver) in a sero-diagnostic assay (Ab-detection ELISA) for the diagnosis of cystic echinococcosis in cattle and buffalo with much improved sensitivity and specificity.…”

Section: Discussionmentioning

confidence: 99%

“…This notion is strengthened by Ibrahem et al [49] who found that the sensitivity of recombinant-protein-based ELISA (25%) was lower compared to crude/native antigens from camel and sheep (see Table 5). Lower specificity might also arise due to the presence of other infections such as Taenia hydatigena, which can be mistaken as hydatid cysts, but would not have been detectable in an HCFantigen-based ELISA [41]. Gatti et al [37] used three forms of antigens (LHT, S2B and B) in their enzyme immunoassay (EIA) and recorded the sensitivity and specificity of each (see Table 4), concluding that either LHT or S2B should be used for the sero-diagnosis of cystic echinococcosis.…”

Section: Discussionmentioning

confidence: 99%

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IEg67 kDa Bovine Hydatid Cyst Antigen: A Candidate for Developing Sero-Diagnostic Assays for Cystic Echinococcosis, a Disease of One Health Importance

Cable

Younus

Rashid

et al. 2023

Animals

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Cystic echinococcosis (hydatidosis) is a world-wide zoonotic disease of mainly humans, livestock and dogs, caused by Echinococcus granulosus. The disease can negatively impact food production and animal welfare and causes socio-economic hardship. Here, we aimed to identify the local bovine hydatid cyst fluid (BHCF) antigen for developing a sero-diagnostic assay to be used for the pre-slaughter screening of food animals. In total, 264 bovines approved for slaughter in Pakistan were subjected to serum collection and post-mortem screening for hydatid cysts. These cysts were assessed microscopically to assess fertility and viability, and by PCR for molecular confirmation of species. A BHCF antigen was identified from positive sera via SDS-PAGE, confirmed by Western blot, and quantified via a bicinchoninic acid (BCA) assay. The quantified crude BHCF antigen (iEg67 kDa) was then used in ELISA screening to test all sera collected from known positive and negative animals based on hydatid cyst presence/absence. Of the 264 bovines examined, 38 (14.4%) showed hydatid cysts during post-mortem examination. All of these individuals, plus an additional 14 (total: 52; 19.6%) tested positive based on less time-consuming ELISA examination. Based on ELISA, occurrence in females (18.8%) was significantly higher than in males (9.2%) and was higher in cattle (19.5%) compared to buffalo (9.5%). The infection rate increased with age in both host species: cumulatively, 3.6% in animals aged 2–3 years, 14.6% in 4–5-year-olds and 25.6% in 6–7-year-olds. The occurrence of cysts in cattle was significantly higher in the lungs (14.1%) compared to their livers (5.5%), whereas the opposite was true in buffalo (6.6% livers, 2.9% lungs). For both host species, most cysts in the lungs were fertile (65%), while the majority in the liver were sterile (71.4%). We conclude that the identified iEg67 kDa antigen is a strong candidate for the development of a sero-diagnostic screening assay for the pre-slaughter diagnosis of hydatidosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

IEg67 kDa Bovine Hydatid Cyst Antigen: A Candidate for Developing Sero-Diagnostic Assays for Cystic Echinococcosis, a Disease of One Health Importance

Cable

Younus

Rashid

et al. 2023

Animals

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“…(2015) Camels 125 55 (44.0) Liver, lung Kandil et al. (2019) Camels 528 93 (17.6) Liver, lung El-Kattan et al. (2020) Camels 180 (18.9) na Mahdy et al (2014) Buffaloes na (16.0) na El Kordy (1946) Buffaloes na (0) Lung, liver Rahman et al (1992) Cattle na (10.0) na El Kordy (1946) Cattle 1114 (0.3) na Hamdy et al.…”

Section: Studies On Cystic Echinococcosis In Egyptmentioning

confidence: 99%

“…Among all livestock, a higher prevalence rate was frequently detected in camels than in other animals. For instance, in Menofia and Cairo, prevalence rates of 17.6–44.0% have been reported in camel herds ( El-Meleh et al., 2019 ; El-Bahy et al., 2019 ; Kandil et al., 2019 ; El-Kattan et al., 2020 ). This observation could be attributed to the importation of camels from countries bordering Egypt, such as Libya and Sudan where CE is endemic and the pastoral system of camels breeding along with nearby dogs exist ( Omar et al., 2013 ; El-Dakhly et al., 2019 ; Abbas et al, 2020 ).…”

Section: Studies On Cystic Echinococcosis In Egyptmentioning

confidence: 99%

Cystic echinococcosis in humans and animals in Egypt: An epidemiological overview

Abdelbaset

Yagi

Nonaka

2021

Current Research in Parasitology & Vector-Borne Diseases

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Animal hydatid cyst genotypes as a potential substitute for human hydatid cyst as a source of antigen for diagnosis of zoonotichydatidosis

et al. 2020

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The Diagnosis of hydatidosis is still an unsolved issue due to difficulties in obtaining of patient's hydatid cyst appropriate for antigen extraction. This study evaluated the suitability of HC protoscolices somatic antigens (HCPsS-Ag) fractions from animal origin to substitute that extracted from HC of patients in diagnosis of hydatidosis using enzyme-linked immunoelectro-transfer blot and Enzyme-linked immunosorbent assay (ELISA). Eight fractions in HC-G6 from patients react specifically versus HC-G6 infected patient's sera. Five of them (28, 32, 38, 59 and 89 Kilo Dalton (KDa) and two of them (28 KDa and 45 KDa) reacted versus HC-G1 and HC-G4 infected sheep and equine sera, respectively. Six fractions in HCPsS-Ag-G1 of sheep react versus HC-G1 sheep infected sera, four (28, 32, 52 and 58 KDa) and two of them reacted versus HC-G6 and HC-G4 infected patient and equine sera, respectively. Two fractions only in HCPsS-Ag-G4 of equine reacted versus infected human and sheep sera. This fraction displayed the same degree of ELISA value versus different infected sera with a significantly perfect classification for kappa agreement and non-statistically significant difference (p C 0.05) for ELISA Optical density values of the positive samples without cross-reaction versus other parasites antibodies in sera. HCPsS-Ag from HC genotypes that developed in humans and animals as HC-G6 and HC-G1 can substitute each other for diagnosis of infection than antigens extracted from non-zoonotic HC-G4. The fraction at 28 KDa is the only fraction that can be extracted from any animals HC and used in diagnosis of zoonotic hydatidosis.

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Sensitivity and specificity of Indirect Enzyme Linked Immuno sorbent Assay (ELISA) for diagnosis of hydatidosis in dromedary camels using hydatid cyst fluid antigens.

Cited by 3 publications

References 14 publications

IEg67 kDa Bovine Hydatid Cyst Antigen: A Candidate for Developing Sero-Diagnostic Assays for Cystic Echinococcosis, a Disease of One Health Importance

IEg67 kDa Bovine Hydatid Cyst Antigen: A Candidate for Developing Sero-Diagnostic Assays for Cystic Echinococcosis, a Disease of One Health Importance

Cystic echinococcosis in humans and animals in Egypt: An epidemiological overview

Animal hydatid cyst genotypes as a potential substitute for human hydatid cyst as a source of antigen for diagnosis of zoonotichydatidosis

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