Sensitivity and Regulation of Diel Photosynthesis in Red-Fleshed Pitaya (Hylocereus polyrhizus) Micropropagules under Mannitol-Induced Water Stress/Rehydration Cycle In Vitro

Abstract

Climate change-induced prolonged water stress (WS) affects crassulacean acid metabolism photosynthesis in pitaya (Hylocereus), limiting crop productivity through insufficient photosynthate. To document how WS/rehydration affects diel photosynthesis, red-fleshed pitaya (H. polyrhizus) micropropagules were studied for 5 weeks in a mannitol-induced water potential gradient replaced with moderate (MWS; −1.0 MPa in week 2; −0.5 MPa for the rest) or intensified (IWS; −1.0 and −1.5 MPa in weeks 2 and 3; −0.5 MPa for the rest) WS in vitro. Net photosynthetic rate (Pn) and integrated net CO2 uptake (INCU) were measured using an Arduino-based photosynthesis system. Micropropagules under MWS had similar Pn in weeks 5 and 1, whereas the control (−0.5 MPa) increased. Pn recovery did not occur after IWS. The average relative INCU was similar in the control and MWS, but lower in IWS. The Pn difference increased with WS, becoming more evident at dawn (Phase II), evening (Phase IV), and predawn the next day (Phase I), and occurred earlier in Phases IV and I under IWS. MWS did not reduce photosynthesis, demonstrating that the photosynthetic regulation could respond to short-term WS in pitaya and indicating the potential of watering for Pn recovery at evening and predawn under IWS.