Sensitivity and Detection Efficiency of a Novel Two-Step Detection System (PowerVision) for Immunohistochemistry

Shi, Shan‐Rong; Guo, James; Côté, Richard J.; Young, Lillian; Hawes, Debra; Shi, Yan; Thu, Sandra; Taylor, Clive R.

doi:10.1097/00129039-199909000-00005

Cited by 46 publications

(35 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The key reagent for higher sensitivity is the anti-mouse-HRP polymer (Powervision), which has a high peroxidase enzyme content as compared with the small size of the polymer molecule. The HRP-polymer has recently been shown to be extremely sensitive in antibody immunohistochemistry (Shi et al, 1999). With the HRP-polymer-based detection, we estimate that the sensitivity of the probe detection in CISH is close to that of FISH, in which no special signal enhancements are usually needed.…”

Section: Discussionmentioning

confidence: 91%

Amplification of c-myc Oncogene by Chromogenic and Fluorescence In Situ Hybridization in Archival Breast Cancer Tissue Array Samples

Rummukainen

Salminen

Lundin

et al. 2001

Laboratory Investigation

View full text Add to dashboard Cite

SUMMARY:Fluorescence in situ hybridization (FISH) is currently considered to be the most specific and sensitive method for detection of oncogene amplifications in human tumor samples. However, FISH requires fluorescence microscopy, which is tedious and does not allow histopathologic evaluation of the cells and tissues examined. Here we compared FISH with the newly developed chromogenic in situ hybridization (CISH), which uses peroxidase enzyme for probe detection instead of fluorescent dyes. CISH was found to be highly concordant with FISH in a tissue array series of 177 archival breast cancer samples. This was true both when comparing CISH with single-color and two-color FISH, the latter including the chromosome 8 centromere probe as reference (the kappa coefficients were 0.67 and 0.76, respectively). Clinicopathologic correlations of c-myc amplification as detected by FISH and CISH were generally the same. By both methods, c-myc amplification was significantly associated with high histologic grade, negative progesterone receptor status, DNA aneuploidy, and high S-phase fraction. c-myc amplification was strongly associated with poor distant metastasis-free survival when amplification was detected by CISH (p ϭ 0.0013), but this association was weaker when FISH was used (p ϭ 0.16 for two-color FISH and p ϭ 0.065 for single-color FISH). These data suggest that CISH is at least as sensitive and specific as FISH in the detection of oncogene amplification in human tumor samples. The possibility for concomitant tissue architecture evaluation using an ordinary transmitted light microscope may favor the use of CISH over FISH in oncogene amplification detection in large tumor series, and tissue arrays and, ultimately, in routine clinical diagnostics. (Lab Invest 2001, 81:1545-1551.

show abstract

Section: Discussionmentioning

confidence: 91%

Amplification of c-myc Oncogene by Chromogenic and Fluorescence In Situ Hybridization in Archival Breast Cancer Tissue Array Samples

Rummukainen

Salminen

Lundin

et al. 2001

Laboratory Investigation

View full text Add to dashboard Cite

show abstract

“…149,411 Its advantages are (1) simplicity compared with the 3-step methods, (2) equal or higher sensitivity than ABC or LSAB methods, and (3) lack of background due to endogenous biotin or avidin. 269,318,335,370,398 However, this method is usually more expensive than ABC or LSAB methods (Fig. 15).…”

Section: Avidin-biotin Methodsmentioning

confidence: 99%

When Tissue Antigens and Antibodies Get Along

2013

View full text Add to dashboard Cite

Once focused mainly on the characterization of neoplasms, immunohistochemistry (IHC) today is used in the investigation of a broad range of disease processes with applications in diagnosis, prognostication, therapeutic decisions to tailor treatment to an individual patient, and investigations into the pathogenesis of disease. This review addresses the technical aspects of immunohistochemistry (and, to a lesser extent, immunocytochemistry) with attention to the antigen-antibody reaction, optimal fixation techniques, tissue processing considerations, antigen retrieval methods, detection systems, selection and use of an autostainer, standardization and validation of IHC tests, preparation of proper tissue and reagent controls, tissue microarrays and other high-throughput systems, quality assurance/quality control measures, interpretation of the IHC reaction, and reporting of results. It is now more important than ever, with these sophisticated applications, to standardize the entire IHC process from tissue collection through interpretation and reporting to minimize variability among laboratories and to facilitate quantification and interlaboratory comparison of IHC results.

show abstract

“…After that, hypophyseal sections were immunohistochemically stained with the polyclonal rabbit anti-human growth hormone (hGH) antibody (Code A0570, Dako Cytomation, Glostrup, Denmark, 1:100) using streptavidin-biotin method (LSAB ® 2 System-HRP; DAKO A/S, Glostrup, Denmark) for the detection of the anterior pituitary's somatotropes (Sternberger et al, 1970;Milosevic et al, 2002). Immunohistochemical analysis of the psoas major muscle sections was performed by means of the UltraVision LP Detection System HRP Polymer and DAB Plus Chromogen detection system using mouse monoclonal anti -fast myosin (MY-32) antibody (Clone MYSN02, Ready to Use, Thermo Scientific TM Lab Vision, CA, 1:320) for the detection of type 2 muscle fibers (Shi et al, 1999;Petrosyan et al, 2002). Histological analysis of the stained sections was performed by Olympus CHK2 light microscope under the 40× lens magnification.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Morphometric analysis of somatotropic cells of the adenohypophysis and muscle fibers of the psoas muscle in the process of aging in humans

Antić

Stefanović

Jovanović

et al. 2015

Annals of Anatomy - Anatomischer Anzeiger

View full text Add to dashboard Cite

Sensitivity and Detection Efficiency of a Novel Two-Step Detection System (PowerVision) for Immunohistochemistry

Cited by 46 publications

References 18 publications

Amplification of c-myc Oncogene by Chromogenic and Fluorescence In Situ Hybridization in Archival Breast Cancer Tissue Array Samples

Amplification of c-myc Oncogene by Chromogenic and Fluorescence In Situ Hybridization in Archival Breast Cancer Tissue Array Samples

When Tissue Antigens and Antibodies Get Along

Morphometric analysis of somatotropic cells of the adenohypophysis and muscle fibers of the psoas muscle in the process of aging in humans

Contact Info

Product

Resources

About