Sensitivity Analysis of a Nuclear Electroporation Model—A Theoretical Study

Costa, Jorge Alberto Vieira; Oliveira, Pedro Xavier de; Pereira, Lucenara S.; Rodrigues, Jéssica Ferreira; Suzuki, Daniela Ota Hisayasu

doi:10.1109/tdei.2021.009456

Cited by 5 publications

(1 citation statement)

References 35 publications

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“…COMSOL Multiphysics ® version 4.4 software is used for FEM modeling and 2D electric currents application ('ec', from the COMSOL's AC/DC Module). The electroporation model is solved in the time domain (as described previously [4,31]), giving results of pore density N(t) and average membrane electrical conductivity (see Appendix A Equations (A1) and (A2)). The simulation steps are defined as linear and were 0.5 µs when using 50 µs PEF and 0.01 µs when using 1 µs PEF.…”

Section: Numerical Modelingmentioning

confidence: 99%

Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation

Pintarelli

Silva

Yang

et al. 2022

Sensors

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A specific pulsed electric field protocol can be used to induce electroporation. This is used in the food industry for yeast pasteurization, in laboratories for generic transfer and the medical field for cancer treatment. The sensing of electroporation can be done with simple ‘instantaneous’ voltage-current analysis. However, there are some intrinsic low-frequency phenomena superposing the electroporation current, such as electrode polarization. The biological media are non-homogeneous, giving them specific characterization in the broad frequency spectrum. For example, the cell barrier, i.e., cell membrane, causes so called β-dispersion in the frequency range of tens to thousands of kHz. Electroporation is a dynamic phenomenon characterized by altering the cell membrane permeability. In this work, we show that the impedance measurement at certain frequencies could be used to detect the occurrence of electroporation, i.e., dielectric dispersion modulated sensing. This approach may be used for the design and implementation of electroporation systems. Yeast suspension electroporation is simulated to show changes in the frequency spectrum. Moreover, the alteration depends on characteristics of the system. Three types of external buffers and their characteristics are evaluated.

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Section: Numerical Modelingmentioning

confidence: 99%

Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation

Pintarelli

Silva

Yang

et al. 2022

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

Simulation of microfluidic intracellular delivery based on the synergy of cell squeezing and electrical field

Chen,

Liu,

et al. 2023

Microfluid Nanofluid

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Higher dose makes higher lethality? A dose–response model of pulsed electric fields inactivation from multiscale coarse-graining method

Wu,

Li,

Chen

et al. 2024

Journal of Applied Physics

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As an emerging technology in liquid inactivation, one of the main challenges of pulsed electric fields (PEFs) inactivation lies in quantitatively describing and predicting its lethality to microorganisms. However, due to its cross-scaled complexity and the consequent numerous regulatory factors, there is currently still no unified framework to understand the PEF dose–response relationship and the population dynamics theoretically. In this study, a simple yet powerful model from multiscale coarse-graining method is proposed to simulate the bacterial inactivation in suspensions during PEF processing. The complex dose–response effects at the macroscale are successfully reconstructed from simple evolution rules and several coarse-graining parameters, while considering the damage and death of a single bacterium at the microscale. Our model uncovers the seemingly chaotic and even controversial dose–response relationship of PEF in literatures and systematically explores the regulatory effect of experimental parameters in a unified framework. One of the interesting findings is that PEF with shorter pulsed width enhances lethality and reduces the minimal inhibitory time at a constant energy output per pulse, owing to the phase transitions in three bacterial population dynamics (Bistability mode, Avalanche mode, and Hybrid mode). Our study provides a new insight for numerically modeling PEF lethality in liquid inactivation and could serve as a guide for dosage management in practical applications.

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Sensitivity Analysis of a Nuclear Electroporation Model—A Theoretical Study

Cited by 5 publications

References 35 publications

Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation

Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation

Simulation of microfluidic intracellular delivery based on the synergy of cell squeezing and electrical field

Higher dose makes higher lethality? A dose–response model of pulsed electric fields inactivation from multiscale coarse-graining method

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