Sensitivity Analysis for Ultra-Wideband 2-Port Impedance Spectroscopy of a Live Cell

Ma, Xiao; Du, Xiaotian; Li, Lei; Li, Hang; Cheng, Xuanhong; Hwang, J.C.M.

doi:10.1109/jerm.2019.2921221

Cited by 14 publications

(9 citation statements)

References 18 publications

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“…In general, whether a cell is treated or not, Δ| S 21 | increases with increasing frequency and Δ| S 21 | < Δ| S 11 |. This is consistent with the theoretical analysis [ 25 ], independent of the calibration technique.…”

Section: Resultssupporting

confidence: 88%

“…The S parameters are deembedded from the as-measured S parameters shown in Figure 2 using the calibrated error matrixes X −1 and Y −1 as described in the above. It can be seen that the deembedded S -parameter magnitudes agree with the simulated values of Figure 6 a, whereas the deembedded S -parameter phases are too small and noisy to be useful as seen in previous experiment [ 13 ] and analysis [ 25 ].…”

Section: Calibration Technique and Standardssupporting

confidence: 73%

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Broadband Electrical Sensing of a Live Biological Cell with In Situ Single-Connection Calibration

et al. 2020

Sensors

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Single-connection in situ calibration using biocompatible solutions is demonstrated in single-cell sensing from 0.5 to 9 GHz. The sensing is based on quickly trapping and releasing a live cell by dielectrophoresis on a coplanar transmission line with a little protrusion in one of its ground electrodes. The same transmission line is used as the calibration standard when covered by various solutions of known permittivities. The results show that the calibration technique may be precise enough to differentiate cells of different nucleus sizes, despite the measured difference being less than 0.01 dB in the deembedded scattering parameters. With better accuracy and throughput, the calibration technique may allow broadband electrical sensing of live cells in a high-throughput cytometer.

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Section: Resultssupporting

confidence: 88%

Section: Calibration Technique and Standardssupporting

confidence: 73%

Broadband Electrical Sensing of a Live Biological Cell with In Situ Single-Connection Calibration

et al. 2020

Sensors

Self Cite

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“…Combined with the use and the manipulation of small liquid volumes, this sensing technique has been successfully developed for molecular and cellular analysis [4][5][6]. The technique has been applied to tissues, cells suspensions and down to individual cells analysis [7,8]. It was also demonstrated for cell quantification, identification and the monitoring of biological processes, while cells were maintained in their traditional environment, their culture medium.…”

Section: Introductionmentioning

confidence: 99%

Microwave dielectric spectroscopy of individual electroporated cells

Tamra

Grenier

Rols

et al. 2021

2021 XXXIVth General Assembly and Scientific Symposium of the International Union of Radio Science (URSI GASS)

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This paper illustrates the interest of analyzing electroporated cells with microwave dielectric spectroscopy. Different electroporation conditions have been applied on cells, for which individual microwave characterization leads to not only quantitative capacitive spectra with similar results as with the trypan blue standard viability test, but with a possible fine graduation of the electroporation impact on a cell population at the single cell level.

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“…Firstly demonstrated with tissues, microwave spectroscopy was demonstrated for cell quantification, pathological state discrimination and even cell identification [4,5], for cells suspensions and also at the single cell level [6,7].…”

Section: Introductionmentioning

confidence: 99%

Non-invasive real time monitoring of cellular modifications with microwave dielectric spectroscopy at the single cell level

Grenier

Chen

Dubuc

2021

2021 XXXIVth General Assembly and Scientific Symposium of the International Union of Radio Science (URSI GASS)

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Microwave dielectric spectroscopy constitutes an attractive sensing technique for cellular analysis. One key feature is its ability to non-invasively and dielectrically monitor cellular modifications at the single cell level. The proposed microwave sensor dedicated to single cell measurement includes a mechanical trap, which permits to apply on individual cells whatever liquid-based environment. The dielectric consequences on the cell due to the application of saponine at 0.02% is recorded every two minutes, demonstrating the possibility to reach dielectric transformation kinetic of cells. This proof of concept opens the door to the non-invasive and nondestructive evaluation of biological reactions at the cellular level.

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Sensitivity Analysis for Ultra-Wideband 2-Port Impedance Spectroscopy of a Live Cell

Cited by 14 publications

References 18 publications

Broadband Electrical Sensing of a Live Biological Cell with In Situ Single-Connection Calibration

Broadband Electrical Sensing of a Live Biological Cell with In Situ Single-Connection Calibration

Microwave dielectric spectroscopy of individual electroporated cells

Non-invasive real time monitoring of cellular modifications with microwave dielectric spectroscopy at the single cell level

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