Sensitive, simple staining method for use in electrophoretic determination of amylase isoenzymes in serum.

The origin, mechanism, and significance of the bile duct proliferation (BDP) associated with cholestasis remain unexplained. This study examined the effect of oral administration of ursodeoxycholic acid (UDCA) on both BDP and cholestasis in the rat. After bile duct ligation, male Sprague-Dawley rats were treated for 30 days with either UDCA (5 mg/day) (group A) or saline solution (group B). Animals were sacrificed at day 30. The serum activity of aminotransferase (ALT, AST), alkaline phosphatase, and gamma-glutamyltransferase (GGT) was significantly lower (P < 0.01) in the UDCA-treated rats. Total serum bilirubin and total serum bile acids were lower (P < 0.001) in group A. Moreover, the control of BA in bile was reduced also (P < 0.02). Conversely, serum cholesterol levels were not different between the two groups. Histological examination showed that the number of ductular cells in the portal areas was significantly (P < 0.001) reduced in UDCA-treated as compared to saline-treated rats. The replication activity, assessed as the number of bromodeoxyuridine-positive cells, was also significantly lower in treated animals (33 +/- 11 vs 64 +/- 22 per 1000 cells; P < 0.001). Lobular bile ductules were three times larger in group B, and extrahepatic duct measurements confirmed this increase in size of the larger biliary ducts (P < 0.001). These findings demonstrate that UDCA reduces BDP in response to BD ligation. Although the mechanism(s) of this effect is still hypothetical, UDCA may reduce the level of irritating bile salts such as chenodeoxycholic acid and lithocolate and increase periductular bile acid recirculation.(ABSTRACT TRUNCATED AT 250 WORDS)

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Effect of ursodeoxycholic acid administration on bile duct proliferation and cholestasis in bile duct ligated rat

Frezza

Gerunda

Plebani

et al. 1993

Digest Dis Sci

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Sources of variation analysis and derivation of reference intervals for ALP, LDH, and amylase isozymes using sera from the Asian multicenter study on reference values

Shimizu

Ichihara

2015

Clinica Chimica Acta

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Alpha-Amylase Inhibitor, CS-1036 Binds to Serum Amylase in a Concentration-Dependent and Saturable Manner

et al. 2013

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(2R,3R,4R)-4-hydroxy-2-(hydroxymethyl)pyrrolidin-3-yl 4-O-(6-deoxyb-D-glucopyranosyl)-a-D-glucopyranoside (CS-1036), which is an a-amylase inhibitor, exhibited biphasic and sustained elimination with a long t 1/2 (18.4-30.0 hours) in rats and monkeys, but exhibited a short t 1/2 (3.7-7.9 hours) in humans. To clarify the species differences in the t 1/2 , the plasma protein binding of CS-1036 was evaluated by ultrafiltration. A concentration-dependent and saturable plasma protein binding of CS-1036 was observed in rats and monkeys with the dissociation rate constant (K D ) of 8.95 and 27.2 nM, and maximal binding capacity (B max ) of 52.8 and 22.1 nM, respectively. By the assessments of the recombinant amylase and immunoprecipitation, the major binding protein of CS-1036 in rats was identified as salivary amylase (K D 5.64 nM). CS-1036 also showed concentration-dependent and saturable binding to human salivary and pancreatic amylase, with similar binding affinity in rats. However, the protein binding of CS-1036 was constant in human plasma (£10.2%) due to the lower serum amylase level compared with rats and monkeys. From the calculation of the unbound fraction (fu) in plasma based on in vitro K D and B max , the dose-dependent increase in fu after oral administration is speculated to lead to a dose-dependent increase in total body clearance and a high area under the curve/dose at lower doses, such as 0.3 mg/kg in rats.

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Sensitive, simple staining method for use in electrophoretic determination of amylase isoenzymes in serum.

Cited by 3 publications

References 3 publications

Effect of ursodeoxycholic acid administration on bile duct proliferation and cholestasis in bile duct ligated rat

Effect of ursodeoxycholic acid administration on bile duct proliferation and cholestasis in bile duct ligated rat

Sources of variation analysis and derivation of reference intervals for ALP, LDH, and amylase isozymes using sera from the Asian multicenter study on reference values

Alpha-Amylase Inhibitor, CS-1036 Binds to Serum Amylase in a Concentration-Dependent and Saturable Manner

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