Sensitive SERS nanotags for use with a hand-held 1064 nm Raman spectrometer

Kearns, Hayleigh; Ali, Fatima; Bedics, Matthew A.; Shand, Neil C.; Faulds, Karen; Detty, Michael R.; Graham, Duncan

doi:10.1098/rsos.170422

Cited by 15 publications

(17 citation statements)

References 39 publications

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“…Secondly, the step-by-step imaging mode realizes rapid movement of the laser spot by the rotation of galvo mirrors, which greatly reduces the scanning time. Thirdly, data processing line-by-line in parallel in SWIFT mode also helps time saving 57–59 . More importantly, three Raman spectra randomly selected from different parts (point 1–3 in Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultrabright gap-enhanced Raman tags for high-speed bioimaging

et al. 2019

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Surface-enhanced Raman spectroscopy (SERS) is advantageous over fluorescence for bioimaging due to ultra-narrow linewidth of the fingerprint spectrum and weak photo-bleaching effect. However, the existing SERS imaging speed lags far behind practical needs, mainly limited by Raman signals of SERS nanoprobes. In this work, we report ultrabright gap-enhanced Raman tags (GERTs) with strong electromagnetic hot spots from interior sub-nanometer gaps and external petal-like shell structures, larger immobilization surface area, and Raman cross section of reporter molecules. These GERTs reach a Raman enhancement factor beyond 5 × 10 9 and a detection sensitivity down to a single-nanoparticle level. We use a 370 μW laser to realize high-resolution cell imaging within 6 s and high-contrast (a signal-to-background ratio of 80) wide-area (3.2 × 2.8 cm 2 ) sentinel lymph node imaging within 52 s. These nanoprobes offer a potential solution to overcome the current bottleneck in the field of SERS-based bioimaging.

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Section: Resultsmentioning

confidence: 99%

Ultrabright gap-enhanced Raman tags for high-speed bioimaging

et al. 2019

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“…For many applications, fluorescence is a major problem and the most efficient method to contrast it consists in working with excitations in the near infrared. Therefore, the development of substrates able to enhance Raman in that region is a relevant topic [21,525,526,527]. SERS as such possesses recognition capabilities; however, when the analyte is mixed in very complex matrices, it could be difficult to extract its signal due to the interference of other compounds.…”

Section: Discussionmentioning

confidence: 99%

A Review on Surface-Enhanced Raman Scattering

et al. 2019

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Surface-enhanced Raman scattering (SERS) has become a powerful tool in chemical, material and life sciences, owing to its intrinsic features (i.e., fingerprint recognition capabilities and high sensitivity) and to the technological advancements that have lowered the cost of the instruments and improved their sensitivity and user-friendliness. We provide an overview of the most significant aspects of SERS. First, the phenomena at the basis of the SERS amplification are described. Then, the measurement of the enhancement and the key factors that determine it (the materials, the hot spots, and the analyte-surface distance) are discussed. A section is dedicated to the analysis of the relevant factors for the choice of the excitation wavelength in a SERS experiment. Several types of substrates and fabrication methods are illustrated, along with some examples of the coupling of SERS with separation and capturing techniques. Finally, a representative selection of applications in the biomedical field, with direct and indirect protocols, is provided. We intentionally avoided using a highly technical language and, whenever possible, intuitive explanations of the involved phenomena are provided, in order to make this review suitable to scientists with different degrees of specialization in this field.

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“…A significant amount of work in advancing SERS tags into the second NIR window has been performed by Graham and colleagues. They have designed hollow gold nanoshells and resonant Raman reporters to generate SERS tags with strong enhancements at wavelengths greater than 1000 nm [48,50,61,62]. Specifically, their SERS tags were able to achieve picomolar detection sensitivities using both 1280 nm and 1550 nm excitation wavelengths [48,61].…”

Section: Sers In the Second Nir Windowmentioning

confidence: 99%

“…Specifically, their SERS tags were able to achieve picomolar detection sensitivities using both 1280 nm and 1550 nm excitation wavelengths [48,61]. Recently, they were able to demonstrate designs achieving 4–5 fM detection limits using a hand-held device operating with 1064 nm excitation at 30 mW using 0.5-s acquisition [62]. These particles are promising, as they are bright enough to be useful for in-vivo spectroscopic detection.…”

Section: Sers In the Second Nir Windowmentioning

confidence: 99%

“…As there are a wide variety of anisotropic particles with strong resonances in the NIR spectral window, the push towards increased sensitivity for SERS has shifted towards Raman reporter development [6,48,62]. Though there are numerous dyes that can be used for SERS tags having high sensitivities in the first NIR window, the number of dyes optimized for the second window are far fewer.…”

Section: Prospects and Future Directionsmentioning

confidence: 99%

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Emergence of two near-infrared windows for in vivo and intraoperative SERS

Lane

Xue

Nie

2018

Current Opinion in Chemical Biology

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Two clear windows in the near-infrared (NIR) spectrum are of considerable current interest for in vivo molecular imaging and spectroscopic detection. The main rationale is that near-infrared light can penetrate biological tissues such as skin and blood more efficiently than visible light because these tissues scatter and absorb less light at longer wavelengths. The first clear window, defined as light wavelengths between 650nm and 950nm, has been shown to be far superior for in vivo and intraoperative optical imaging than visible light. The second clear window, operating in the wavelength range of 1000-1700nm, has been reported to further improve detection sensitivity, spatial resolution, and tissue penetration because tissue photon scattering and background interference are further reduced at longer wavelengths. Here we discuss recent advances in developing biocompatible plasmonic nanoparticles for in vivo and intraoperative surface-enhanced Raman scattering (SERS) in both the first and second NIR windows. In particular, a new class of 'broad-band' plasmonic nanostructures is well suited for surface Raman enhancement across a broad range of wavelengths allowing a direct comparison of detection sensitivity and tissue penetration between the two NIR window. Also, optimized and encoded SERS nanoparticles are generally nontoxic and are much brighter than near-infrared quantum dots (QDs), raising new possibilities for ultrasensitive detection of microscopic tumors and image-guided precision surgery.

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Sensitive SERS nanotags for use with a hand-held 1064 nm Raman spectrometer

Cited by 15 publications

References 39 publications

Ultrabright gap-enhanced Raman tags for high-speed bioimaging

Ultrabright gap-enhanced Raman tags for high-speed bioimaging

A Review on Surface-Enhanced Raman Scattering

Emergence of two near-infrared windows for in vivo and intraoperative SERS

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