“…ELISA has been demonstrated to be effective for the detection of several viruses present in the seeds, such as Alfalfa mosaic virus (Pesic and Hiruke, 1986), Barley stripe mosaic virus (Lister et al, 1981), Bean common mosaic virus (Jafarpour et al, 1979), Broad bean stain virus (Makkouk et al, 1987), Cucumber mosaic virus (Jones and Proudlove, 1991), Cucumber green mottle virus (Faris-Mukhayyish and Makkouk, 1983), Melon necrotic spot virus (Avegelis and Barba, 1986), Pea seed-borne mosaic virus (Hamilton and Nicholas, 1978;Ding et al, 1992), Pea early browning virus (van Vuurde and Maaqt, 1985), Peanut mottle virus (Bharathan et al, 1984), Peanut stripe virus Culver and Sherwood, 1988), Prune dwarf virus and Prune necrotic ringspot virus (Mink and Aichele, 1984), Soybean mosaic virus (Maury et al, 1985), Squash mosaic virus (Nolan and Campbell, 1984), and Tobacco ringspot virus (Lister, 1978). Sweet corn seedlings grown from 46,600 seeds were tested by ELISA for the presence of High Plains virus (HPV) and three plants tested positive for HPV, whose occurrence was first observed in 1993 (Forster et al, 2001).…”