Sensitive quantification of coixol, a potent insulin secretagogue, in Scoparia dulcis extract using high‐performance liquid chromatography combined with tandem mass spectrometry and UV detection

Abstract

Diabetes is a major global health problem which requires new studies for its prevention and control. Scoparia dulcis, a herbal product, is widely used for treatment of diabetes. Recent studies demonstrate coixol as a potent and nontoxic insulin secretagog from S. dulcis. This study focuses on developing two quantitative methods of coixol in S. dulcis methanol-based extracts. Quantification of coixol was performed using high-performance liquid chromatography-tandem mass spectrometry (method 1) and high-performance liquid chromatography-ultraviolet detection (method 2) with limits of detection of 0.26 and 11.6 pg/μL, respectively, and limits of quantification of 0.78 and 35.5 pg/μL, respectively. S. dulcis is rich in coixol content with values of 255.5 ± 2.1 mg/kg (method 1) and 220.4 ± 2.9 mg/kg (method 2). Excellent linearity with determination coefficients >0.999 was achieved for calibration curves from 10 to 7500 ng/mL (method 1) and from 175 to 7500 ng/mL (method 2). Good accuracy (bias < -8.6%) and precision (RSD < 8.5%) were obtained for both methods. Thus, they can be employed to analyze coixol in plant extracts and herbal formulations.

Molecular Action Mechanism of Coixol from Soft-Shelled Adlay on Tyrosinase: The Future of Cosmetics

“…The content of coixol in the extracts of coicis seeds was determined according to Ali et al (2017) [ 54 ]. In brief, the HPLC analyses of the standard coixol and coicis extracts were performed on Agilent 1200 SL Rapid Resolution HPLC-UV system (Agilent Technologies, Singapore).…”

“…Solution A (0.1% TFA in water, v/v ) and solution B (methanol) were operated in a linear gradient manner: 15% B from 0–1 min, 15–50% B from 1–2 min, 50–95% B from 2–6 min, maintained at 95% 6–7 min, and 95–15% B from 7–8 min. The column temperature was maintained at 30 °C [ 54 ].…”

Acute and Prolonged Effects of a Polyherbal Formulation on Blood Glucose, Lipid Profile and Liver Function in Normal and Streptozotocin-Induced Diabetic Rats

“…[34] [36] [38] [65] [66][67], suggesting that the wide variety of chemical classes among the many extracted substances of each plant indicates probable involvement of variety of mechanisms in the blood glucose reduction as reported byGushiken et al (2016) [68]. Constituents of these hypoglycemic plants as Scoparic acid D, Coxoil and Glutinol from S. dulcis[15] [69][70], some flavonoids, terpenes as saponins and alkaloids from all of them are known to stimulate β cells insulin secretion in vitro and/or in vivo, to inhibit hepatic gluconeogenesis and intestinal glucose absorption trough synthesis modulation of intestinal and hepatic enzymes involved in carbohydrates metabolism, to stimulate hepatic glycogenesis, to stimulate peripheral glucose uptake and utilization, and residual pancreatic β cells regeneration[11] [38] [40] [41] [43] [44] [45] [71] [72] [73]. The insulinopenia due to β cells damage after STZ administration and the consequent hyperglycemia also subsequently contribute to the serum creatinine increase as observed in DC rats compared to NC rats.…”