Sensitive QD@SiO2-based immunoassay for triplex determination of cereal-borne mycotoxins

Beloglazova, Natalia V.; Foubert, Astrid; Gordienko, Anna; Tessier, Martine; Aubert, Tangi; Drijvers, Emile; Goryacheva, Irina Yu.; Hens, Zeger; Saeger, Sarah De

doi:10.1016/j.talanta.2016.05.015

Cited by 29 publications

(10 citation statements)

References 28 publications

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“…QDs have a wide excitation, narrow emission spectra, high quantum yield, and exceptional photochemical stability. This approach has been described for immunohistochemistry (Stroh et al, 2005), immunoassays (Beloglazova et al, 2014, 2016) and detection of proteins in the biological samples (Qiu et al, 2017; Shu and Tang, 2017; Zhang et al, 2017). These nanoparticles contribute high specificity, substantial surface area and stability to the miRNA nano-biosensors.…”

Section: Determination Of Circulating Cell Free Mirnamentioning

confidence: 99%

Quantum Dot Based Nano-Biosensors for Detection of Circulating Cell Free miRNAs in Lung Carcinogenesis: From Biology to Clinical Translation

et al. 2018

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Lung cancer is the most frequently occurring malignancy and the leading cause of cancer-related death for men in our country. The only recommended screening method is clinic based low-dose computed tomography (also called a low-dose CT scan, or LDCT). However, the effect of LDCT on overall mortality observed in lung cancer patients is not statistically significant. Over-diagnosis, excessive cost, risks associated with radiation exposure, false positive results and delay in the commencement of the treatment procedure questions the use of LDCT as a reliable technique for population-based screening. Therefore, identification of minimal-invasive biomarkers able to detect malignancies at an early stage might be useful to reduce the disease burden. Circulating nucleic acids are emerging as important source of information for several chronic pathologies including lung cancer. Of these, circulating cell free miRNAs are reported to be closely associated with the clinical outcome of lung cancer patients. Smaller size, sequence homology between species, low concentration and stability are some of the major challenges involved in characterization and specific detection of miRNAs. To circumvent these problems, synthesis of a quantum dot based nano-biosensor might assist in sensitive, specific and cost-effective detection of differentially regulated miRNAs. The wide excitation and narrow emission spectra of these nanoparticles result in excellent fluorescent quantum yields with a broader color spectrum which make them ideal bio-entities for fluorescence resonance energy transfer (FRET) based detection for sequential or simultaneous study of multiple targets. In addition, photo-resistance and higher stability of these nanoparticles allows extensive exposure and offer state-of-the art sensitivity for miRNA targeting. A major obstacle for integrating QDs into clinical application is the QD-associated toxicity. However, the use of non-toxic shells along with surface modification not only overcomes the toxicity issues, but also increases the ability of QDs to quickly detect circulating cell free miRNAs in a non-invasive mode. The present review illustrates the importance of circulating miRNAs in lung cancer diagnosis and highlights the translational prospects of developing QD-based nano-biosensor for rapid early disease detection.

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Section: Determination Of Circulating Cell Free Mirnamentioning

confidence: 99%

Quantum Dot Based Nano-Biosensors for Detection of Circulating Cell Free miRNAs in Lung Carcinogenesis: From Biology to Clinical Translation

et al. 2018

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“…The other way around, where epoxide containing molecules are coupled to QDs-COOH is already published [94]. To our knowledge, we were the first to synthesize and use epoxidefunctionalized silica QDs for the development of a multiplex immunosorbent assay for the simultaneous detection of three mycotoxins [95].…”

Section: Conjugation Of Qds Containing Epoxide Functionalitymentioning

confidence: 99%

Bioconjugation of quantum dots: Review & impact on future application

Foubert

Beloglazova

Rajković

et al. 2016

TrAC Trends in Analytical Chemistry

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117

110

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Nowadays luminescent semiconductor quantum dots (QDs) are widely applied in different areas due to their unique optical properties. QDs can be used as photoluminescent labels with excellent possibilities for high-throughput detection and diagnostics. For most of such applications QDs must be coupled to biomolecules, which often represents a fundamental challenge. Although QDs have a lot of advantages over organic dyes, most of the techniques that have been developed for QD functionalization and bioconjugation, are more complicated than the corresponding techniques for organic fluorescent dyes. Here, the importance of choosing a suitable bioconjugation strategy in different applications, such as imaging and assays is described. The main goal of this review is to give a structured and detailed overview and comparison of the most widely used conjugation strategies in function of the active groups (carboxyl, amine, thiol, epoxy, hydroxyl and aldehyde groups) present on QD surface.

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“…Wu et al established multiplexed fluorescence resonance energy transfer aptasensor between upconversion nanoparticles and graphene oxide for the simultaneous determination of OTA and FB1 with the LOD of 0.02 ng/mL for OTA and 0.1 ng/mL for FB 1 [101]. Chen et al applied antigen-modified magnetic nanoparticles as biosensor probes and antibody-functionalized improved upconversion nanoparticles as signal probes to develop an ultrasensitive fluorescence biosensor, which showed the LOD of 0.001 ng/mL and detection linear range of 0.001-0.1 ng/mL for AFB 1 and DON in peanut oils [102]. Saeger group used quantum dot (QD) nanolabels to develop the multiplex fluorescent immunosorbent simultaneous analysis method which has LODs of 3.2, 0.6, 0.2, 10 and 0.4 μg/kg for DON, ZEN, AFB 1 , T-2 and FB1 in maize [103].…”

Section: Other Biosensor Techniques For Multiplex Mycotoxin Analysismentioning

confidence: 99%

High Throughput Detection Methods for Multiplex Mycotoxins

Li¹,

Deng²,

Liu³

et al. 2017

Toxicol Open Access

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Mycotoxins are toxic low-molecular-weight compounds which produced by the metabolism of certain fungi species. Due to their toxicity, chemistry stability, diversity and co-occurrence in agriculture products, it is urgently needed to develop the rapid, simple and effective detection technique methods to monitor and prevent mycotoxin contamination in whole food chain. This article gives a review about high throughput detection methods for multiplex mycotoxins which mainly includes chromatographic instrument techniques, microarray chip, suspension array, lateral flow biosensors, surface plasmon resonance (SPR) and nanoparticle-based biosensors. The advantages and disadvantages and the key steps of them have been discussed. The insight and evaluation of the technique progress were given, which would be helpful to further develop this filed.

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Sensitive QD@SiO2-based immunoassay for triplex determination of cereal-borne mycotoxins

Cited by 29 publications

References 28 publications

Quantum Dot Based Nano-Biosensors for Detection of Circulating Cell Free miRNAs in Lung Carcinogenesis: From Biology to Clinical Translation

Quantum Dot Based Nano-Biosensors for Detection of Circulating Cell Free miRNAs in Lung Carcinogenesis: From Biology to Clinical Translation

Bioconjugation of quantum dots: Review & impact on future application

High Throughput Detection Methods for Multiplex Mycotoxins

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