Sensitive Mercury Speciation Analysis in Water by High-Performance Liquid Chromatography-Atomic Fluorescence Spectrometry Coupling with Solid-Phase Extraction

Abstract

An efficient method based on high performance liquid chromatography coupled with atomic fluorescence spectrometry (HPLC-AFS) was successfully developed for the simultaneous determination of four mercury species including Hg 2+ , methylmercury (MeHg), ethylmercury (EtHg), and phenylmercury (PhHg) in water. Samples were enriched and cleaned up with solid phase extraction (SPE) pretreatment using thiol cartridge, some key parameters including selection of SPE cartridge, eluent type, eluent volume, and the interference factors were systematically investigated. The chromatographic separation was achieved on C 18 column using a mobile phase consisting of methanol and 60 mmol L -1 ammonium acetate with 10 mmol L -1 L-cysteine by gradient elution. Under the optimized conditions, good linearity (r ≥ 0.9991) was observed between 0.20 μg L -1 to 10.

Recent Progress on Fluorescent Probes in Heavy Metal Determinations for Food Safety: A Review

“…The method successfully reduced the detection limits, ranging from 0.8 ng•L −1 for Mn to 0.09 µg•L −1 for Hg, with relative standard deviations all < 5%, and was successfully applied to the determination of heavy metals in rice and rice products. Similarly, D. Chen et al [35] established a simple and efficient method, which combined high-performance liquid chromatography-atomic fluorescence spectrometry (HPLC-AFS) and SPE. The method showed high sorption capacity and a wide range of adaptability for the simultaneous determination of four forms of mercury (Hg 2+ , MeHg, EtHg, PhHg).…”

A Fluorescent Probe for Hg2+ Specific Recognition Based on Xanthene and its Application in Food Detection and Cell Imaging

“…The detection of Hg 2+ has evolved from traditional instrumental analysis (such as spectrophotometry, high-performance liquid chromatography, and inductively coupled plasma emission spectrometry [13][14][15][16] ) to sensing detection in recent years. The sensing analysis method includes the advantages of the sensitivity and accuracy of the traditional detection method, and makes up for the shortcomings of the traditional detection in terms of cost investment, operation technology and practical application.…”

Rhodamine-Based Fluorescent Probe for Highly Selective Determination of Hg2+

“…To date, many analytic approaches have been employed for sensing Hg 2+ such as high-performance liquid chromatography–inductively coupled plasma mass spectrometry (HPLC–ICP-MS), HPLC coupled with atomic fluorescence spectrometry (HPLC–AFS), ICP-atomic emission spectrometry (ICP-AES), atomic absorption spectrometry (AAS), ultraviolet–visible absorption spectrometry (UV–vis), fluorometry, , electrochemical methods, , and so on. Among all the methods developed, fluorometry possess remarkable advantages because of its high sensitivity, inherent simplicity, instrument operability, in situ detection, and bioimaging analysis in vivo. , Hitherto, many fluorescent probes had been constructed to determine Hg 2+ . − However, some analytical performances of these probes are unsatisfactory, including the slow response time, − cross interference, , poor sensitivity, − and so forth.…”