Abstract:DNA repair enzyme ALKBH3 has shown great potential as diagnostic as well as therapeutic target for several cancers. However, methods for the sensitive measurement of ALKBH3 repair activity are highly desirable but challenging. Herein, we report a dual DNA amplifier by integrating catalytic hairpin assembly (CHA) with CRISPR/Cas12a for highly sensitive monitoring of ALKBH3 activity. A 3MeC‐modified initiator DNA was designed as the substrate of ALKBH3. Upon ALKBH3‐mediated DNA repair to remove the lesion from i… Show more
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