Sensitive immunosensor for benzo[a]pyrene detection based on dual amplification strategy of PAMAM dendrimer and amino-modified methylene blue/SiO2 core–shell nanoparticles

Lin, Mouhong; Liu, Yingju; Liu, Chuanhe; Yang, Zhuohong; Huang, Yibin

doi:10.1016/j.bios.2011.02.028

Cited by 29 publications

(5 citation statements)

References 38 publications

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“…The BaP detection limit of a SPR immunosensor by employing a self-assembled mixed monolayer presenting BaP head groups was 50 ppt (about 0.2 nmol·L −1 ) [26]. Based on a dual amplification strategy of PAMAM dendrimer and amino-modified methylene blue/SiO 2 core-shell nanoparticles, a detection limit of 6 pg/mL (about 23.8 pmol·L −1 ) was obtained for the electrochemical immunosensor [27]. An electro-switchable biosurface could detect 10 ng/L (about 40 pmol·L −1 ) BaP by an indirect immunoassay format [28].…”

Section: Resultsmentioning

confidence: 99%

Nanoporous Gold Films Prepared by a Combination of Sputtering and Dealloying for Trace Detection of Benzo[a]pyrene Based on Surface Plasmon Resonance Spectroscopy

Wang

Wan

et al. 2017

Sensors

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A wavelength-interrogated surface plasmon resonance (SPR) sensor based on a nanoporous gold (NPG) film has been fabricated for the sensitive detection of trace quantities of benzo[a]pyrene (BaP) in water. The large-area uniform NPG film was prepared by a two-step process that includes sputtering deposition of a 60-nm-thick AuAg alloy film on a glass substrate and chemical dealloying of the alloy film in nitric acid. For SPR sensor applications, the NPG film plays the dual roles of analyte enrichment and supporting surface plasmon waves, which leads to sensitivity enhancement. In this work, the as-prepared NPG film was first modified with 1-dodecanethiol molecules to make the film hydrophobic so as to improve BaP enrichment from water via hydrophobic interactions. The SPR sensor with the hydrophobic NPG film enables one to detect BaP at concentrations as low as 1 nmol·L−1. In response to this concentration of BaP the sensor produced a resonance-wavelength shift of ΔλR = 2.22 nm. After the NPG film was functionalized with mouse monoclonal IgG1 that is the antibody against BaP, the sensor’s sensitivity was further improved and the BaP detection limit decreased further down to 5 pmol·L−1 (the corresponding ΔλR = 1.77 nm). In contrast, the conventional SPR sensor with an antibody-functionalized dense gold film can give a response of merely ΔλR = 0.9 nm for 100 pmol·L−1 BaP.

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Section: Resultsmentioning

confidence: 99%

Nanoporous Gold Films Prepared by a Combination of Sputtering and Dealloying for Trace Detection of Benzo[a]pyrene Based on Surface Plasmon Resonance Spectroscopy

Wang

Wan

et al. 2017

Sensors

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“…Mainly electrochemical immunosensors have attracted considerable interest due to their advantages, such as simple pretreatment procedure, fast analytical time, low detection limits, low cost, and the possibility of miniaturization [ 17 ]. Measurements in a voltammetric immunosensor are based on the application of constant potential at the working electrode, which is in close proximity to the reference electrode, and the current is registered due to electrochemical oxidation or reduction of an investigated electroactive species [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Strategy for Selective Thyroid Hormone Determination Based on an Electrochemical Biosensor with Graphene Nanocomposite

Baluta

Romaniec

Alicka³

et al. 2023

Sensors

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This article presents a novel and selective electrochemical bioassay with antibody and laccase for the determination of free thyroid hormone (free triiodothyronine, fT3). The biosensor was based on a glassy carbon electrode modified with a Fe3O4@graphene nanocomposite with semiconducting properties, an antibody (anti-PDIA3) with high affinity for fT3, and laccase, which was responsible for catalyzing the redox reaction of fT3. The electrode modification procedure was investigated using a cyclic voltammetry technique, based on the response of the peak current after modifications. All characteristic working parameters of the developed biosensor were analyzed using differential pulse voltammetry. Obtained experimental results showed that the biosensor revealed a sensitive response to fT3 in a concentration range of 10–200 µM, a detection limit equal to 27 nM, and a limit of quantification equal to 45.9 nM. Additionally, the constructed biosensor was selective towards fT3, even in the presence of interference substances: ascorbic acid, tyrosine, and levothyroxine, and was applied for the analysis of fT3 in synthetic serum samples with excellent recovery results. The designed biosensor also exhibited good stability and can find application in future medical diagnostics.

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“…Another example of a dendrimer-layered immunosensor architecture was described by Lin and co-workers, which covalently attached PAMAM G-2.0 dendrimer on glassy carbon electrodes previously coated with an electrodeposited film of graphene/chitosan to construct an amperometric immunosensor for benzo[a]pyrene [50]. In addition, these authors constructed a competitive immunosensor for the same analyte by using Au electrodes coated with electropolymerized 2-amino-5,2 :5 2 -terthiophene and PAMAM G-2.0 dendrimer as transducers, and methylene blue@SiO 2 core@shell nanoparticle loaded with horseradish peroxidase and a secondary antibody as the labeling element [51].…”

mentioning

confidence: 99%

Dendrimers as Soft Nanomaterials for Electrochemical Immunosensors

et al. 2019

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Electrochemical immunosensors are antibody-based affinity biosensors with a high impact on clinical, environmental, food, and pharmaceutical analysis. In general, the analytical performance of these devices is critically determined by the materials and reagents used for their construction, signal production and amplification. Dendrimers are monodisperse and highly branched polymers with three-dimensional structures widely employed as “soft” nanomaterials in electrochemical immunosensor technology. This review provides an overview on the state-of-the-art in dendrimer-based electrochemical immunosensors, focusing on those using polyamidoamine and poly (propylene imine) dendrimers. Special emphasis is given to the most original methods recently reported for the construction of immunosensor architectures incorporating dendrimers, as well as to novel sensing approaches based on dendrimer-assisted signal enhancement strategies.

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Sensitive immunosensor for benzo[a]pyrene detection based on dual amplification strategy of PAMAM dendrimer and amino-modified methylene blue/SiO2 core–shell nanoparticles

Cited by 29 publications

References 38 publications

Nanoporous Gold Films Prepared by a Combination of Sputtering and Dealloying for Trace Detection of Benzo[a]pyrene Based on Surface Plasmon Resonance Spectroscopy

Nanoporous Gold Films Prepared by a Combination of Sputtering and Dealloying for Trace Detection of Benzo[a]pyrene Based on Surface Plasmon Resonance Spectroscopy

A Novel Strategy for Selective Thyroid Hormone Determination Based on an Electrochemical Biosensor with Graphene Nanocomposite

Dendrimers as Soft Nanomaterials for Electrochemical Immunosensors

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