Sensitive immunochromatographic assay for the detection of the dimethachlone fungicide in tomatoes and lettuces

Abstract

Here, we describe a sensitive and rapid gold nanoparticle-based immunochromatographic strip (GNP-ICS) for the detection of dimethachlone (DMT) in tomatoes and lettuces using GNP-labeled monoclonal antibodies (mAbs) as the probe....

Rapid and sensitive detection of chlordimeform in cucumber and tomato samples using an immunochromatographic assay

“…The synthesis of colloidal gold was carried out by the sodium citrate reduction method. 33,34 Briefly, 0.01% HAuCl 4 (50 mL) was brought to boil. We added 1% trisodium citrate (1 mL) and continued to stir the solution until it turned wine-red (15 min).…”

Monoclonal antibody production and development of immunochromatographic strip assays for screening of the herbicide bispyribac-sodium in rice

“…Spleen cells from the selected mice were fused with myeloma cells (SP2/0) to generate hybridoma cell lines as previously reported. 17,18 Aer four rounds of subcloning using the limited gradient dilution method, the hybridoma cell lines against BIS were screened. [19][20][21] The selected hybridoma cell lines were injected into a mouse aer expanding in culture.…”