Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid

Katz, J. I.; Alstad, Arnold D.; Gustafson, G A; Moser, Kristina

doi:10.1128/jcm.31.11.3028-3030.1993

Cited by 45 publications

(12 citation statements)

References 11 publications

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“…All PCRs were carried out with denaturation at 94°C for 15 s, annealing at 55°C for 30 s and extension at 68°C with one minute for every thousand bases of the PCR product. The isolate was confirmed as BTV by Seg‐5‐ and Seg‐7‐based conventional RT‐PCR as described earlier (Katz et al., ; OIE, , Wade‐Evans et al., ). Initially, IND2010/01 cDNA was subjected to serotype‐specific RT‐PCR for eight common serotypes identified during the last decade from India (Reddy et al., ), followed by primers against 26 global serotypes of BTV (Maan et al., ) to identify the serotype of the isolate.…”

Section: Methodsmentioning

confidence: 90%

Isolation of Bluetongue Virus 24 from India - An Exotic Serotype to Australasia

Krishnajyothi

Maan

Kandimalla

et al. 2016

Transbound Emerg Dis

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Bluetongue (BT) is a viral disease of ruminants and is caused by different serotypes of bluetongue virus (BTV), which is transmitted by several species of Culicoides midges. The disease is endemic in tropical areas, and incursions have been observed in some of the temperate areas. Twenty-seven recognized serotypes of BTV have been reported so far. Some serotype viruses have been shown to circulate in certain geographical areas. BTV-24 has been reported from Africa, the Mediterranean and the Americas, whereas it is exotic to Australasia. Here, we report isolation of BTV-24 from India and show that it has high sequence homology in genome segment 2 with other Western isolates of BTV-24. Entry of this serotype into Australasian region is a cause of concern.

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Section: Methodsmentioning

confidence: 90%

Isolation of Bluetongue Virus 24 from India - An Exotic Serotype to Australasia

Krishnajyothi

Maan

Kandimalla

et al. 2016

Transbound Emerg Dis

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“…Previously published primers were used for NS1 and VP7 genes (Wade‐Evans et al., 1990; Katz et al., 1993; OIE, 2010), and for VP2 , NS2 and VP6 genes, primers were designed using VP2, NS2 and VP6 sequences of USA BTV‐10 vaccine virus using NCBI‐Primer Blast (Rozen and Skaletsky, 2000) program (Table 1). Infected cell lysate was subjected to centrifugation, and RNA was extracted from pelleted cells and membranes using Trizol‐LS ® (Invitrogen Bioservices Private Limited, Bangalore, India), denatured at 65°C for 10 min and snap cooled on ice.…”

Section: Methodsmentioning

confidence: 99%

Sequences of Genes Encoding Type-Specific and Group-Specific Antigens of an Indian Isolate of Bluetongue Virus Serotype 10 (BTV-10) and Implications for their Origin

Gollapalli

Mallavarapu²,

Uma³

et al. 2011

Transboundary and Emerging Diseases

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Bluetongue, a transboundary disease, is endemic in several tropical countries and is caused by bluetongue virus (BTV). The origin and movement of BTV can be predicted by comparing nucleotide sequences of its segmented RNA genome. Such analyses have been useful in evaluating the source of the virus responsible for recent incursion of BTV into previously unreported areas. Besides several serotypes, genetically related BTV strains circulate in each endemic area, but such clusters of strains have been reported to be distinct from one geographical region to another. We obtained partial or complete sequences of the open reading frames encoded by VP2, VP6, VP7, NS1 and NS2 genes of a BTV-10 isolate of India and compared them with other BTV-10 sequences available in public database. Sequences of all the five genes showed >99% identity to BTV-10 prototype, vaccine strain and vaccine-like virus isolates from the USA. Our results suggest that Indian BTV-10 virus analysed in this study possibly originated from the United States.

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“…Recently, several investigators have presented sensitive assays for the diagnosis of various viral (16,17,24,30,35), bacterial (3,5,7,8,20,22,23,28,33,37), fungal (14), and protozoal (2) infections on the basis of nonradioactive (colorimetric or chemiluminescent) nucleic acid hybridization. These kinds of techniques are simple, and colorimetry requires only a conventional microtiter spectrophotometric reader and can be readily done on a large scale.…”

mentioning

confidence: 99%

Evaluation of performances of three DNA enzyme immunoassays for detection of Helicobacter pylori PCR products from biopsy specimens

1997

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PCR is recognized as a promising method for the detection of Helicobacter pylori in gastric biopsy specimens. However, detection of PCR products by gel electrophoresis is difficult to implement in routine clinical laboratories. The aim of this study was to compare three new DNA enzyme immunoassays with the standard method in their ability to detect PCR products. The three assays were based on the amplification of a fragment of the ureC gene of H. pylori and a colorimetric hybridization assay. The first assay (GEN-ETI-K DNA enzyme immunoassay; Sorin, Sallugia, Italy) was based on the hybridization of amplified DNA with a probe bound in microtiter wells and detected with labelled anti-DNA antibody. The second assay (Pylori-prob; Biocode, Sclessin, Belgium) comprised a solid-phase sandwich hybridization system with a specific biotinylated probe being used for detection. Finally, the third assay (PCR enzyme-linked immunosorbent assay; Boehringer, Mannheim, Germany) was based on the hybridization of amplified DNA labelled with digoxigenin as a probe (used as a coating in microtiter wells) and detected with antidigoxigenin-peroxidase as conjugate. The sensitivity of the colorimetric assay was evaluated by using amplification products from PCR assays performed on several 10-fold dilutions of DNA from H. pylori CIP 101260, and the specificity was assessed with different urease-positive bacteria. Biopsy specimens from 199 patients were tested; 106 were classified as H. pylori positive, and 93 were classified as H. pylori negative by culture and/or histological examination as the "gold standard." The receiving operating characteristic curve was used to determine the best cutoff point for each assay. The detection of PCR products by colorimetric hybridization increases the sensitivity up to 100-fold compared to that with gel electrophoresis. The results are rapid (4 h) and easy to interpret and can be automated.

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Sensitive identification of bluetongue virus serogroup by a colorimetric dual oligonucleotide sorbent assay of amplified viral nucleic acid

Cited by 45 publications

References 11 publications

Isolation of Bluetongue Virus 24 from India - An Exotic Serotype to Australasia

Isolation of Bluetongue Virus 24 from India - An Exotic Serotype to Australasia

Sequences of Genes Encoding Type-Specific and Group-Specific Antigens of an Indian Isolate of Bluetongue Virus Serotype 10 (BTV-10) and Implications for their Origin

Evaluation of performances of three DNA enzyme immunoassays for detection of Helicobacter pylori PCR products from biopsy specimens

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