Sensitive glucometer-based microfluidic immune-sensing platform via DNA signal amplification coupled with enzymatic reaction

A novel β-cyclodextrin-assisted enhancement strategy for portable and sensitive detection of miR-21 in human serum

“…[18][19][20] This further application of a PGM is highly cost-effective and greatly expands the range of applications for POCT. [21][22][23][24] The main challenge in using a PGM for the detection of targets other than glucose is that the detection limit of a PGM can't meet the requirements of most analytes. 25 Therefore, the signal transduction and ampli-cation are particularly important.…”

A dual-mode strategy based on β-galactosidase and target-induced DNA polymerase protection for transcription factor detection using colorimetry and a glucose meter

“…[23][24][25] Representative PGMbased strategies often combine target amplification with signal transduction to achieve sensitive and specific detection. [25][26][27] As the signal transduction involved transducing the non-glucose substrates into glucose, only several enzymes, including α-glucosidase, invertase, alkaline phosphatase (ALP), hexokinase, and glucose oxidase, have been chosen to turn the substrates into glucose. [28][29][30][31] Furthermore, the PGM-based signal can be influenced by test conditions, detection surroundings, and device efficiency.…”