Sensitive Glass Microfibre‐Based Histamine Analysis for Allergy Testing in Washed Blood Cells

Ps, Skov; Mosbech, H.; Norn, S.; Weeke, B.

doi:10.1111/j.1398-9995.1985.tb00219.x

Cited by 103 publications

(34 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fibers were inserted through 2 cm of the cutis of the volar forearm under local anesthesia (Emla, AstraZeneca) and perfused with sterile isotonic saline solution at a rate of 3.0 µl/min. Histamine concentrations were determined by fluorescence spectroscopy [9] in the microdialysis eluate obtained consecutively for 24 (12 × 2) min each at baseline, after cold stimulation (ice cube) and after intracutaneous injection of 50 µg of codeine 1 mm away from the fiber. …”

Section: Methodsmentioning

confidence: 99%

Cold Urticaria Patients Exhibit Normal Skin Levels of Functional Mast Cells and Histamine after Tolerance Induction

et al. 2012

View full text Add to dashboard Cite

Cold urticaria is a skin condition characterized by rapid appearance of itchy wheals and occasionally angioedema in response to cold stimulation. Antihistamines do not sufficiently protect all patients from symptoms, even when used in higher than standard doses. In these patients, desensitization to cold can be beneficial. The aim was to investigate whether desensitization can lower temperature thresholds and reduce release of histamine in the skin. Cold urticaria patients were subjected to desensitization and assessed for skin responses to cold stimulation and codeine before and after. Histamine levels mediated by cold and codeine were determined by cutaneous microdialysis before and after desensitization in patients and healthy controls. Desensitization to cold resulted in protection from cold-induced symptoms and lower temperature thresholds in six out of nine patients. Desensitization also prevented histamine release after skin exposure to cold. Surprisingly, skin histamine levels and release after codeine injection were found to be normal in desensitized patients.

show abstract

Section: Methodsmentioning

confidence: 99%

Cold Urticaria Patients Exhibit Normal Skin Levels of Functional Mast Cells and Histamine after Tolerance Induction

et al. 2012

View full text Add to dashboard Cite

show abstract

“…After pas sive sensitization, this dependence disappeared and no significant difference in the maximum anti-IgE-in- duced HR was seen between the three plgE levels. Furthermore, the dose response to anti-IgE became similar to that observed in adult blood, exhibiting the well-desribed bell shape [13,14] with maximum re lease occurring at a tenfold lower concentration of anti-lgE than before passive sensitization.…”

Section: Ige-mediated Histamine Releasementioning

confidence: 96%

“…Only sam ples containing more than 8.5 ml of blood were used for the passive sensitization and pharmacological modulation experiments (n = 86). Histamine Release Determination HR was measured by a fluorometric method based on the affin ity of histamine for ground glass microfibers, modified from that first described by Stahl Skov et al [13,14].…”

Section: Blood Samplingmentioning

confidence: 99%

See 1 more Smart Citation

Author Index

1990

Int Arch Allergy Immunol

View full text Add to dashboard Cite

“…A previously described micromethod was applied [15]. The provoc ative concentration was determined as the concentration giving half the maximum histamine release.…”

Section: Histamine Release Assaymentioning

confidence: 99%

Modification of House Dust Mite Allergens by Monomethoxypolyethylene Glycol

Mosbech

Dreborg

Påhlman³

et al. 1988

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

In animal models, allergen modification by coupling to monomethoxypolyethylene glycol (mPEG) molecules can reduce allergenicity of the extract and makes the allergen capable of suppressing boosted IgE response. To investigate in a human system the degree of attenuation implied by a mPEG modification of a house dust mite (Dermatophagoides pteronyssinus) extract, 55 adults with asthma caused by house dust mites were tested by skin prick test (SPT) and histamine release assay (HR). RAST inhibition was performed on sera from 6 additional patients. Modified extract containing 0.42 mmol mPEG/g protein was used for the analyses. In order to get the same response of the two extracts when assessed by HR and SPT, a median increase in concentration of 10-fold of the mPEG-modified extract compared to the unmodified extract was needed. Interindividual variation was limited. Sixty-four to 72% needed a dose increase within ± half a decade from this value. In 42–49% of the patients, results from SPT and HR deviated less than half a decade. The relative potency of the modified extract as measured by RAST inhibition was reduced to 17–78% (mean 39%). Reduced allergenicity would by itself mean less side effects in immunotherapy. When planning such therapy it is important to know that mPEG modification reduces the allergenicity to a similar extent in a majority of patients.

show abstract

Sensitive Glass Microfibre‐Based Histamine Analysis for Allergy Testing in Washed Blood Cells

Cited by 103 publications

References 12 publications

Cold Urticaria Patients Exhibit Normal Skin Levels of Functional Mast Cells and Histamine after Tolerance Induction

Cold Urticaria Patients Exhibit Normal Skin Levels of Functional Mast Cells and Histamine after Tolerance Induction

Author Index

Modification of House Dust Mite Allergens by Monomethoxypolyethylene Glycol

Contact Info

Product

Resources

About