Sensitive electrogenerated chemiluminescence biosensors for protein kinase activity analysis based on bimetallic catalysis signal amplification and recognition of Au and Pt loaded metal-organic frameworks nanocomposites

Yan, Zhiyong; Wang, Feng; Deng, Pingye; Wang, Yu; Cai, Kai; Chen, Yanhui; Wang, Zonghua; Liu, Yang

doi:10.1016/j.bios.2018.03.004

Cited by 64 publications

(20 citation statements)

References 43 publications

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“…Nevertheless, the complicated assembly procedures limit their applications. Recently, a metal–organic framework (MOF) of UiO-66 encapsulated with Ru(bpy) 3 2+ was also applied for the design of an ECL biosensor for kinase activity based on the chelation between the Zr 4+ defects on the surface of UiO-66 and the phosphorylated peptide, which avoids the limited point-to-point recognition between the phosphate group with Zr 4+ or antibody. The poor conductivity of the MOF as well as the high background and toxicity of the coreactor of tripropylamine restrict the performances of these biosensors.…”

Section: Introductionmentioning

confidence: 99%

Integrating Highly Efficient Recognition and Signal Transition of g-C₃N₄ Embellished Ti₃C₂ MXene Hybrid Nanosheets for Electrogenerated Chemiluminescence Analysis of Protein Kinase Activity

Sun

Zhang

et al. 2020

Anal. Chem.

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Herein, a novel electrogenerated chemiluminescence (ECL) method for highly sensitive kinase activity and inhibitors screening was developed based on graphitic carbon nitride nanosheet (g-C3N4) nanoparticle embellished titanium carbide (Ti3C2) MXene nanosheets, integrating the highly efficient recognition and signal amplification activity. In this strategy, kemptide was first immobilized onto a gold electrode, and it was phosphorylated by the protein kinase A which was used as a model. The g-C3N4-MXene probe can adsorb explicitly onto the electrode based on chelation between the phosphate group and Ti defect sites enriched in MXenes. Besides the abundant active sites on MXene for the highly efficient binding with phosphate groups, the Ti3C2 MXene not only provides a unique conductive platform to accommodate more g-C3N4 nanoparticles but also facilitates the electron transfer on the electrode interface and suppresses the passivation of g-C3N4 that explores their highly efficient ECL emission with high stability. The obtained ECL signals were closely related to the kinase activity and can be applied for protein kinase A (PKA) activity detection. Under the optimal conditions, the proposed ECL biosensor provided a quantitative readout to PKA concentration in the range 0.015–40 U mL–1 with the detection limit of 1.0 mU mL–1. The ECL biosensor was also successfully applied to monitor drug-triggered kinase activation and quantitative analysis of the kinase inhibitors in MCF-7 cell lysates. This work shows that the proposed ECL biosensor has the potential to be a powerful tool for PKA study and clinical diagnostics as well as the discovery of new targeted drugs.

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Section: Introductionmentioning

confidence: 99%

Integrating Highly Efficient Recognition and Signal Transition of g-C₃N₄ Embellished Ti₃C₂ MXene Hybrid Nanosheets for Electrogenerated Chemiluminescence Analysis of Protein Kinase Activity

Sun

Zhang

et al. 2020

Anal. Chem.

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“…Recently, metal–organic frameworks (MOFs) have emerged as a type of new material and widely explored for sensor design owing to their high surface areas, flexible porosity, chemical tenability, and durable sensing features. − The high porosity of MOFs offers an ability to accommodate a large number of electroactive molecules, and enhances their conductivity and analysis sensibility. , Particularly, Zr-MOFs have a high affinity to phosphate groups and have been used to selectively enrich phosphates biomolecules via the formation of Zr–O–P bonds. − As we know, exosomes are composed of phospholipid bilayers whose outer surface is surrounded with hydrophilic phosphate head. Inspired by these, we have proposed a label-free and enzyme-free electrochemical biosensor in this work for the analysis of GBM-derived exosomes relied on the interaction between the Zr–O clusters and intrinsic phospholipid bilayer of exosomes.…”

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confidence: 99%

An Electrochemical Biosensor Designed by Using Zr-Based Metal–Organic Frameworks for the Detection of Glioblastoma-Derived Exosomes with Practical Application

et al. 2020

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Glioblastoma (GBM) is one of the most fatal tumors in the brain, and its early diagnosis remains technically challenging due to the complex repertoires of oncogenic alterations and blood-brain barrier (BBB). GBM-derived specific exosomes can cross the BBB and circulate in body fluids, so they can be noninvasive biomarkers for the early diagnosis of GBM. Herein, we propose a sensitive and label-free electrochemical biosensor designed by using Zr-based metal–organic frameworks (Zr-MOFs) for the detection of GBM-derived exosomes with practical application. In the design, a peptide ligand can specifically bind with human epidermal growth factor receptor (EGFR) and EGFR variant (v) III mutation (EGFRvIII), which are overexpressed on the GBM-derived exosomes. Meanwhile, Zr-MOFs encapsulated with methylene blue can absorb on the surface of the exosomes due to the interaction between Zr4+ and the intrinsic phosphate groups outside of exosomes. Consequently, the concentration of exosomes can be directly quantified by monitoring the electroactive molecules inside MOFs, ranging from 9.5 × 103 to 1.9 × 107 particles/μL with the detection of limit of 7.83 × 103 particles/μL. Furthermore, this proposed biosensor can distinguish GBM patients from healthy groups, demonstrating the great prospect for early clinical diagnosis.

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“…The introduction of a bimetallic organic framework enriches the sensor design. In 2018, Yan et al proposed Au&Pt@UiO-66 to detect PKA activity and inhibitor screening ( Yan et al, 2018 ). UiO-66 as a carrier inhibited metal nanoparticle aggregation.…”

Section: Application Of Uio-66-based Electrochemical Biosensorsmentioning

confidence: 99%

Research Progress of UiO-66-Based Electrochemical Biosensors

Zhang

et al. 2022

Front. Chem.

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UiO-66, as a member of the MOFs families, is widely employed in sensing, drug release, separation, and adsorption due to its large specific surface area, uniform pore size, easy functionalization, and excellent stability. Especially in electrochemical biosensors, UiO-66 has demonstrated excellent adsorption capacity and response signal, which significantly improves the sensitivity and specificity of detection. However, the existing application research remains in its infancy, lacking systematic methods, and recycling utilization and exclusive sensing of UiO-66 still require further improvement. Therefore, one of the present research objectives is to explore the breakthrough point of existing technologies and optimize the performance of UiO-66-based electrochemical biosensors (UiO-66-EBs). In this work, we summarized current experimental methods and detection mechanisms of UiO-66-EBs in environmental detection, food safety, and disease diagnosis, analyzed the existing problems, and proposed some suggestions to provide new ideas for future research.

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Sensitive electrogenerated chemiluminescence biosensors for protein kinase activity analysis based on bimetallic catalysis signal amplification and recognition of Au and Pt loaded metal-organic frameworks nanocomposites

Cited by 64 publications

References 43 publications

Integrating Highly Efficient Recognition and Signal Transition of g-C₃N₄ Embellished Ti₃C₂ MXene Hybrid Nanosheets for Electrogenerated Chemiluminescence Analysis of Protein Kinase Activity

Integrating Highly Efficient Recognition and Signal Transition of g-C₃N₄ Embellished Ti₃C₂ MXene Hybrid Nanosheets for Electrogenerated Chemiluminescence Analysis of Protein Kinase Activity

An Electrochemical Biosensor Designed by Using Zr-Based Metal–Organic Frameworks for the Detection of Glioblastoma-Derived Exosomes with Practical Application

Research Progress of UiO-66-Based Electrochemical Biosensors

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Sensitive electrogenerated chemiluminescence biosensors for protein kinase activity analysis based on bimetallic catalysis signal amplification and recognition of Au and Pt loaded metal-organic frameworks nanocomposites

Cited by 64 publications

References 43 publications

Integrating Highly Efficient Recognition and Signal Transition of g-C3N4 Embellished Ti3C2 MXene Hybrid Nanosheets for Electrogenerated Chemiluminescence Analysis of Protein Kinase Activity

Integrating Highly Efficient Recognition and Signal Transition of g-C3N4 Embellished Ti3C2 MXene Hybrid Nanosheets for Electrogenerated Chemiluminescence Analysis of Protein Kinase Activity

An Electrochemical Biosensor Designed by Using Zr-Based Metal–Organic Frameworks for the Detection of Glioblastoma-Derived Exosomes with Practical Application

Research Progress of UiO-66-Based Electrochemical Biosensors

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Integrating Highly Efficient Recognition and Signal Transition of g-C₃N₄ Embellished Ti₃C₂ MXene Hybrid Nanosheets for Electrogenerated Chemiluminescence Analysis of Protein Kinase Activity

Integrating Highly Efficient Recognition and Signal Transition of g-C₃N₄ Embellished Ti₃C₂ MXene Hybrid Nanosheets for Electrogenerated Chemiluminescence Analysis of Protein Kinase Activity