Sensitive electrochemical sequential enzyme biosensor for glucose and starch based on glucoamylase- and glucose oxidase-controllably co-displayed yeast recombinant

Liu, Junchong; Lang, Qiaolin; Liang, Bo; Zheng, Zongmei; Zhang, Yaru; Liu, Aihua

doi:10.1016/j.aca.2022.340173

Cited by 8 publications

(2 citation statements)

References 36 publications

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“…The suggested biosensor was not affected by any of the interference factors. Thus, yeast-GOx/RGO/GCE could be used for sensitive starch and glucose detection in real samples [ 123 ]. Some selected studies about enzyme-based electrochemical nanobiosensors are tabulated in Table 1 .…”

Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

Kilic,

Singh,

Keles

et al. 2023

Biosensors

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Electrochemistry is a genuinely interdisciplinary science that may be used in various physical, chemical, and biological domains. Moreover, using biosensors to quantify biological or biochemical processes is critical in medical, biological, and biotechnological applications. Nowadays, there are several electrochemical biosensors for various healthcare applications, such as for the determination of glucose, lactate, catecholamines, nucleic acid, uric acid, and so on. Enzyme-based analytical techniques rely on detecting the co-substrate or, more precisely, the products of a catalyzed reaction. The glucose oxidase enzyme is generally used in enzyme-based biosensors to measure glucose in tears, blood, etc. Moreover, among all nanomaterials, carbon-based nanomaterials have generally been utilized thanks to the unique properties of carbon. The sensitivity can be up to pM levels using enzyme-based nanobiosensor, and these sensors are very selective, as all enzymes are specific for their substrates. Furthermore, enzyme-based biosensors frequently have fast reaction times, allowing for real-time monitoring and analyses. These biosensors, however, have several drawbacks. Changes in temperature, pH, and other environmental factors can influence the stability and activity of the enzymes, affecting the reliability and repeatability of the readings. Additionally, the cost of the enzymes and their immobilization onto appropriate transducer surfaces might be prohibitively expensive, impeding the large-scale commercialization and widespread use of biosensors. This review discusses the design, detection, and immobilization techniques for enzyme-based electrochemical nanobiosensors, and recent applications in enzyme-based electrochemical studies are evaluated and tabulated.

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Section: Application Of Enzyme-based Electrochemical Nanobiosensorsmentioning

confidence: 99%

Novel Approaches to Enzyme-Based Electrochemical Nanobiosensors

Kilic,

Singh,

Keles

et al. 2023

Biosensors

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“…Hence, the detection of glucose in blood can save lives and control the condition of thousands of people with diabetes. However, most commercial sensors today are still mainly glucose sensors that use the glucose oxidase (Gox) enzyme [1][2][3][4][5][6]. The use of enzymatic sensors requires a prolonged time for sample preparation, technical efforts, and a number of chemicals for immobilizing GOx on the sensor's surface.…”

Section: Introductionmentioning

confidence: 99%

Electrodeposited Fabrication of CeO2 Branched-like Nanostructure Used for Nonenzymatic Glucose Biosensor

Nguyet,

Tuan,

Ngan

et al. 2023

Crystals

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The fabrication of nonenzymatic glucose sensors is essential because of the enhancement in the selectivity and accuracy of these sensors. In this work, we used the electrodeposition approach to prepare a CeO2-based electrode for nonenzymatic glucose detection. A CeO2 branched-like nanostructure was successfully fabricated by electrodeposition on the surface of a Au substrate electrode at room temperature. The effects of cyclic voltammetry, CH3COOH content, and scan cycle number on the formation of the CeO2 branched-like nanostructure were investigated. The fabricated electrodes were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The results showed that a CeO2 branched-like nanostructure could be obtained with a CH3COOH content of 1.0 mL and a scan cycle number of 100 in a solution containing 0.015 M Ce(NO3)3, 0.01 M KCl, and 0.02 M CH3COONH4 and with a scan rate of 400 mV/s. The electrochemical characteristics of the sensor were examined by chronoamperometry and cyclic voltammetry. The results showed that the sensitivity of the sensor was 37.72 μA/mM·cm2 and the limit of detection (LOD) of the sensor was 0.093 mM. The findings in this work prove that it is feasible to fabricate CeO2-based sensors for nonenzymatic glucose detection.

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