Sensitive electrochemical measurement of hydroxyl radical generation induced by the xanthine–xanthine oxidase system

Tatsumi, Hirosuke; Tsuchiya, Yui; Sakamoto, Koichi

doi:10.1016/j.ab.2014.08.028

Cited by 4 publications

(2 citation statements)

References 43 publications

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“…These LOD values are comparable to the results of our previous reports for Cat (8 nM) 14 and 2,6-dimethylhydroquinone (1 nM). 15 The present biosensor was stable at between pH 6 and 8. Within this pH range, the sensitivity increased as the test solution became more alkaline, according to the pH dependence of the activity of PQQ-GDH.…”

Section: Calibration Curvesmentioning

confidence: 71%

“…In our recent study, enzyme-catalyzed signal amplification was introduced to the amperometric detection of the hydroxyl radical. 14,15 In the presence of an aromatic trapping agent, pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH, E.C. 1.1.5.2) as a catalyst, and its substrate D-glucose, the current signal for the trapping adducts from hydroxyl radical was amplified and detected sensitively.…”

Section: Introductionmentioning

confidence: 99%

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Sensitive Simultaneous Determinations of 1,2-Dihydroxynaphthalene and Catechol by an Amperometric Biosensor

et al. 2020

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An amperometric biosensor for 1,2-dihydroxynaphthalene (DHN) and catechol (Cat) has been developed in order to monitor the biodegradaton of polycyclic aromatic hydrocarbons (PAHs). DHN is a common intermediary metabolite in naphthalene and phenanthrene degradation, while Cat is produced by further degradation. These compounds were detected by a biosensor modified with pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH). The biosensor was based on signal amplification by enzyme-catalyzed redox cycling and was able to detect DHN and Cat at very low concentrations down to 10 -9 M. Since the anodic waves of DHN and Cat were well separated, simultaneous determinations of these compounds were possible. Although the current signal for DHN was reduced in repeated measurements due to the oxidative polymerization of DHN, it can be avoided when the concentration of DHN was sufficiently low (<1 μM).

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Section: Calibration Curvesmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%