Sensitive Determination of Urinary Mercury(ii) by a Bioluminescent Transgenic Bacteria-Based Biosensor

Roda, Aldo; Pasini, Patrizia; Mirasoli, Mara; Guardigli, Massimo; Russo, Carmela; Musiani, Monica; Baraldini, M.

doi:10.1081/al-100002702

Cited by 24 publications

(13 citation statements)

References 40 publications

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“…Although mercury is bound within the amalgam, it leaches out in the elemental form Hg(0) as vapor, accounting for 75% of daily mercury exposure. It is then absorbed by the lungs, and oxidized to Hg(II) [41]. Roda et al have optimized a wholecell biosensing system based on E. coli cells, in which the luc reporter gene encoding for firefly luciferase is under the control of a mercury-inducible promoter, for detection of mercury in urine.…”

Section: Mercury(ii) Ionsmentioning

confidence: 99%

Engineered cells as biosensing systems in biomedical analysis

et al. 2012

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Over the past two decades there have been great advances in biotechnology, including use of nucleic acids, proteins, and whole cells to develop a variety of molecular analytical tools for diagnostic, screening, and pharmaceutical applications. Through manipulation of bacterial plasmids and genomes, bacterial whole-cell sensing systems have been engineered that can serve as novel methods for analyte detection and characterization, and as more efficient and cost-effective alternatives to traditional analytical techniques. Bacterial cell-based sensing systems are typically sensitive, specific and selective, rapid, easy to use, low-cost, and amenable to multiplexing, high-throughput, and miniaturization for incorporation into portable devices. This critical review is intended to provide an overview of available bacterial whole-cell sensing systems for assessment of a variety of clinically relevant analytes. Specifically, we examine whole-cell sensing systems for detection of bacterial quorum sensing molecules, organic and inorganic toxic compounds, and drugs, and for screening of antibacterial compounds for identification of their mechanisms of action. Methods used in the design and development of whole-cell sensing systems are also reviewed.

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Section: Mercury(ii) Ionsmentioning

confidence: 99%

Engineered cells as biosensing systems in biomedical analysis

et al. 2012

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“…luciferase and β-galactosidase) that emit visible light in response to the presence of specific substances [72]. Several biosensors using this promoter-reporter-gene concept have been developed for the detection of heavy metals [73,74,75] and toxic organic compounds [76,77,78].…”

Section: Microorganismsmentioning

confidence: 99%

“…Luminescent microbial biosensors for Hg 2+ have been developed [74,75] by use of Escherichia coli cells encoded with firefly luciferase luc as reporter gene under the control of a mercury(II)-inducible promoter. The lowest detectable concentration of Hg 2+ was 0.1 fmol L -1 and no interference from other heavy metal ions has been observed, even at concentrations a million times those of mercury.…”

Section: Microorganismsmentioning

confidence: 99%

Fiber-optic biosensors – an overview

2002

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This article reviews progress and developments during the past five years in the field of optical fiber biosensors. Because of the expense and time constraints associated with modern laboratory analysis, there is a growing need for real-time, low-cost technology that can be used industrially, environmentally, and clinically, and to monitor food processing. Miniaturization, integrated systems, and multianalyte determination have become key aspects of sensor development and efforts in this direction will also be discussed, with some pointers to likely directions of future research in the area. The review will provide information about the analytical characteristics and applications of fiber-optic biosensors classified depending on the biorecognition element employed - enzymes, whole cells, antibodies, nucleic acids, and biomimetic polymers.

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“…Arsenite and arsenate were determined using the GFP reporter gene [114], and biogenic amines were detected with similar technology [115]. The effect of metal toxicity was studied using two different bacteria species [116]; mercury [117] and other heavy metals [25] were detected. Herbicides are targeted using a variety of organisms: Shao et al developed a bioluminescence sensor using cyanobacteria for the detection of herbicides [118].…”

Section: Whole-cell-based Biosensorsmentioning

confidence: 99%

Biosensors for environmental pollutants and food contaminants

Baeumner

2003

Analytical and Bioanalytical Chemistry

214

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This review article provides an overview of the most recent literature on biosensors for environmental pollutants and food contaminants. Due to the large number of publications, only papers published between 2000 and January 2003 were considered. Also, while not all of the published literature could be reviewed here, over 200 references are cited to provide a good overview of research undertaken in the last two years. Older publications are covered by a number of earlier review articles. This article provides an introduction into the field including specific consideration of the application areas, describes the typical biosensor assay format used, and is subsequently structured according to the biorecognition elements used (i.e., nucleic acids, enzymes, whole cells, tissue and whole organisms, antibodies and receptors, and biomimetic materials). In addition, a section on microbiosensing systems is provided. Since only very few microbiosensors with applications in environmental and food systems have been published, enabling technology is also covered in this article.

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Sensitive Determination of Urinary Mercury(ii) by a Bioluminescent Transgenic Bacteria-Based Biosensor

Cited by 24 publications

References 40 publications

Engineered cells as biosensing systems in biomedical analysis

Engineered cells as biosensing systems in biomedical analysis

Fiber-optic biosensors – an overview

Biosensors for environmental pollutants and food contaminants

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