Sensitive determination of l-lysine with a new amperometric microbial biosensor based on Saccharomyces cerevisiae yeast cells

Akyılmaz, Erol; Erdoğan, Ali; Öztürk, Ramazan; Yasa, I Wayan Putu Sutirta

doi:10.1016/j.bios.2006.04.023

Cited by 56 publications

(22 citation statements)

References 30 publications

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“…According to the detection principle, electrochemical techniques (transduction method) can be divided into amperometric (Akyilmaz et al 2007), potentiometric (Ercole et al 2003), conductimetric (Muhammad-Tahir and Alocija 2003a), and impedimetric (Munoz-Berbel et al 2008); these techniques are widely used in the development of microbial biosensors.…”

Section: Conductometric 79 Cfu/mlmentioning

confidence: 99%

Nanobiosensors in Food Science and Technology

Mendoza-Madrigal

Chanona-Pérez

Guadarrama-Fernández

et al. 2015

Food Nanoscience and Nanotechnology

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Section: Conductometric 79 Cfu/mlmentioning

confidence: 99%

Nanobiosensors in Food Science and Technology

Mendoza-Madrigal

Chanona-Pérez

Guadarrama-Fernández

et al. 2015

Food Nanoscience and Nanotechnology

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“…Both are tolerant to liquid exposure and exhibit the high sensitivity needed to detect the small changes of oxygen encountered in cell metabolisms. The first technology, amperiometric electrochemical oxygen sensing, is found in a variety of devices for the detection and identification of different bacteria [4] to microbial biosensors [5]. Despite continuous interest in amperiometric sensors, several significant limitations have been indentified concerning their use with biological systems and microfluidic devices.…”

Section: Oxygen Sensingmentioning

confidence: 99%

Micro-patterning of polymer-based optical oxygen sensors for lab-on-chip applications

Nock

Blaikie

Todem

2007

BioMEMS and Nanotechnology III

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Soft-lithography and plasma etching with reactive ions were used to fabricate a polymer microfluidic cell-culture bioreactor with integrated optical oxygen sensor. Platinum(II) octaethylporphyrin ketone (PtOEPK) suspended in a microporous polystyrene (PS) matrix was spin-coated to form sensor films of variable thickness from 1.1 µm to 400 nm on glass substrates. Sensor films were found to be smooth and well adhered. Arbitrary patterns with a minimum feature size of 25 µm could be routinely replicated in the PtOEPK/PS layer using polydimethylsiloxane (PDMS) elastomer stamps as etch masks in a reactive ion etcher. No effect of plasma patterning and sensor integration by plasma bonding on the sensor signal could be observed. Detection of different gaseous and dissolved oxygen concentrations with the patterned sensor followed linear Stern-Volmer behavior. Dynamic measurement of sensor intensity as a function of different oxygen concentration showed good reproducibility and a nearly instantaneous response to gas changes. For gaseous and dissolved oxygen measurement with a patterned 400 nm thick film I 0 /I 100 ratios of 3.2 and 2.7 were found, respectively.

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“…Amperometric biosensors based on screen printed electrodes have been developed by Sarkar et al [73]. An amperometric microbial biosensor based on Saccharomyces cerevisiae cells was developed for selective and rapid determination of L-lysine [40]. In addition, L-aao was physically immobilized on diamond paste to construct an amperometric biosensor that detects L-leucine by measuring the hydrogen peroxide formed when L-aao catalyses the conversion of L-leucine to their keto acids and H 2 O 2 [74].…”

Section: Applications Of L-aaomentioning

confidence: 99%

“…The Saccharomyces cerevisiae has a specific lysine oxidase activity; however it also accepts L-arginine, L-asparagine and other L-amino acids like alanine, leucine, glutamic acid and tryptophan as substrates [40].…”

Section: Enzymatic Properties Of L-aaosmentioning

confidence: 99%

L-Amino Acid Oxidases-Microbial and Snake Venom

Singh¹

2014

J Microb Biochem Technol

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L-amino acid oxidases (EC 1.4.3.2, are flavoenzymes that catalyse the stereospecific deamination of an L-amino acid to their corresponding α-keto acid with the production of hydrogen peroxide and ammonia. These enzymes are widely distributed across diverse phyla from bacteria, fungi to mammals and many venomous snakes. Although they are mainly involved in cellular amino acid catabolism, many other physiological functions are attributed to L-aao including their antibacterial property and ability to protect from infection. L-aao has also been correlated with penicillin production, violacein synthesis and biofilm development and cell dispersal. Snake venom L-aaos are studied extensively for their ability to induce apoptosis, aggregate platelets, induce haemorrhage, edema and many other toxic effects. L-aaos have been characterized biochemically and found to differ in terms of biochemical parameters not only among different species but also among members of the same species. L-aao act on L-amino acids, preferentially basic, aromatic and aliphatic L-amino acids. The snake venom enzyme shows broad oxidizing activity towards aromatic and hydrophobic L-amino acids such as leucine, phenylalanine and isoleucine.L-aaos have practical value in biochemical and chemical investigations as they have been used to destroy Lisomer of a racemic DL-amino acid and thus yield an optically pure preparation of the D-isomer. As such, L-aao finds numerous applications as catalysts in biotransformation and for production of keto acids. L-aao has also been used for the determination of L-amino acids as part of biosensors. L-amino acids are reported to be found in physiological fluids of patients with certain diseases and disorders. In addition, the content of certain amino acids essentially controls the nutritional quality of the food. L-aao is useful in this aspect by development of biosensors to detect the L-amino acids.Snake venom L-aaos are known to induce apoptosis and antibacterial effects mediated by the hydrogen peroxide produced during the L-aao reaction. The hydrogen peroxide induces oxidative stress which in turn activates the heat shock proteins and initiates an array of functions ultimately leading to apoptosis and cell death. In this aspect, L-aao can be greatly useful for the development of efficient therapeutics and drugs to control tumor cells, bacterial, leishmanicidal, viral and protozoal infections. L-aao is also reported to display dose dependent inhibition on HIV-1 infection and replication and as such can be studied for development of anti HIV medicine.

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Sensitive determination of l-lysine with a new amperometric microbial biosensor based on Saccharomyces cerevisiae yeast cells

Cited by 56 publications

References 30 publications

Nanobiosensors in Food Science and Technology

Nanobiosensors in Food Science and Technology

Micro-patterning of polymer-based optical oxygen sensors for lab-on-chip applications

L-Amino Acid Oxidases-Microbial and Snake Venom

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