Sensitive Determination of Epinephrine Using Kinetic Spectrophotometric Method

Abstract

In this study, thionine -bromate as a sensitive reaction system using kinetic spectrophotometric method for the determination of epinephrine is proposed. The method is based on the catalytic effect of epinephrine on the selected reaction system. The change in absorbance was followed spectrophotometrically as a criterion of the oxidation reaction progress. The effective reaction variables were optimized. Under optimized experimental conditions, calibration curve was linear over the range 0.4 -12.8 mg/l and the detection limit was 0.13 mg/l for five replicate determinations of blank signal. The interfering effect of various species was also investigated. The developed procedure was successfully applied for the determination of epinephrine in pharmaceutical and biological samples satisfactorily.

Simultaneous Electrochemical Determination of Articaine HCl and Epinephrine

“…This allows the local anesthetic to persist at the injection site before being absorbed into the systemic circulation [4]. Literature survey reveals that several different analytical techniques have been developed for the determination of articaine HCl and epinephrine including high performance liquid chromatographic methods [5][6][7], thin layer chromatographic methods [8], gas chromatographic methods [9] and spectrophotometric methods [10] for articaine HCl and high performance liquid chromatographic methods [11][12][13][14][15], thin layer chromatographic methods [16,17], gas chromatographic methods [18,19], spectrophotometric methods [20][21][22][23][24] and electrochemical methods [25][26][27][28][29][30] for epinephrine. Carbon based electrodes are currently in widespread use in electroanalytical chemistry, because of their broad potential window, low cost, rich surface chemistry, low background current and chemical inertness.…”

Spectrofluorimetric determination of adrenaline and dopamine .

“…The effect of dopamine lies in the vital areas of the brain, as it affects mood, sleep, study, focus, and learning, so its deficiency may lead to certain diseases, including; Parkinson's and depression [3][4]. Numerous analytical methods are used to determine adrenaline and dopamine, such as chromatographic methods [5][6][7][8], voltammetry [9][10][11][12][13][14], capillary electrophoresis [15], flow injection [16][17][18], ion-selective electrode [19][20][21][22][23], spectrophotometric [24][25][26][27][28][29][30] and spectrofluorimetric [31][32][33][34] methods, It is known that the fluorescence measurement methods are sensitive, so in this paper, an easy method was described to measure the fluorescence of adrenaline and dopamine and it does not require a lot of materials, that is, it is economically feasible in addition to being in an aqueous medium, while most of the methods used expensive organic solvents, which areharmful to the environment.…”