Sensitive determination of derivatized carbohydrates by high-performance liquid chromatography

Nachtmann, F.; Budna, K. W.

doi:10.1016/s0021-9673(00)86280-9

Cited by 55 publications

(4 citation statements)

References 18 publications

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“…Although the LC separation of aliphatic alcohols is easily achieved by using reversed-phase chromatography, their quantitative determination is hindered due to the lack of an inherent chromophore, fluorophore, or dc-active electrophore. Derivatization to benzoate esters is often used to improve the detection properties of aliphatic alcohols (8,9). On the other hand, reversed-phase chromatography with PAD is highly amenable to the determination of aliphatic alcohols.…”

Section: Introductionmentioning

confidence: 99%

Pulsed amperometric detection of aliphatic alcohols in liquid chromatography

LaCourse¹,

Johnson²,

Rey³

et al. 1991

Anal. Chem.

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The quantitative determination of aliphatic alcohols In liquid chromatography has been hindered by the lack of a sensitive detector. Aliphatic alcohols have no Inherent chromophore or fluorophore and are considered to be electrolnactlve under constant applied potential. The determination of underlvatized aliphatic alcohols by reversed-phase polymer-based chromatography with pulsed amperometric detection (PAD) Is direct, sensitive, and simple. PAD was applied to the determination of simple alcohols at Pt and Au working electrodes under acidic and basic conditions, respectively. The combination of mixed-mode chromatography with a chromophoric (UV-vls) detector and a nonchromophoric detector (PAD) is a powerful analytical technique.

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Section: Introductionmentioning

confidence: 99%

Pulsed amperometric detection of aliphatic alcohols in liquid chromatography

LaCourse¹,

Johnson²,

Rey³

et al. 1991

Anal. Chem.

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“…The reaction replaces the active hydrogens with a nitrobenzoyl group. The method was applicable to mono-, di-, and trisaccharides except fructose (Nachtmann & Budna 1977;Nachtmann 1976). Many of the derivatization reactions for carbohydrates are discussed by Knapp (1979).…”

Section: Other Analytical Techniquesmentioning

confidence: 99%

Extraction and Analysis of Inositols and Other Carbohydrates from Soybean Plant Tissues

Campbell¹,

Goheen²,

Donald³

2011

Recent Trends for Enhancing the Diversity and Quality of Soybean Products

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“…The derivatization of saccharides was performed with 4-NBCl in pyridine according to a modified procedure described by Nachtmann et al 14,15 The reaction was performed in stoppered 10-ml centrifuge tubes. The neutral saccharides present in the samples of the oligogalacturonic acid fractions were derivatized prior to the HPLC analysis.…”

Section: Determination Of Neutral Saccharidesmentioning

confidence: 99%

Characterization and degradation of pectin derived from Budimka apple

Nikolić¹,

Mojović²

2008

JSCS

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The characterization of apple pectin and its oligogalacturonic fractions derived from the autochthones apple variety Budimka, characteristic for central Serbia, is described in this paper. After extraction, the apple pectin was subjected to controlled enzymatic hydrolysis by polygalacturonase (PG) and pectin lyase (PL) from Aspergillus niger and then fractionated by ion-exchange column chromatography on Dowex 1X-8 (200-400 mesh). Saturated oligogalacturonic acids, obtained by controlled hydrolysis with PG, were efficiently separated by elution with a gradient of Na acetate buffer (pH 6.0), while unsaturated oligogalacturonic acids, obtained by controlled hydrolysis with PL, were separated on the same resin, using a gradient of Na formate buffer (pH 4.7) as the eluent. The yields of the fractions with the particular degree of polymerization (DP) were also determined. The total content of neutral saccharides in the original Budimka apple pectin was detected by HPLC analysis of the 4-nitrobenzoyl derivatives of the sugar, and amounted to 5.31 %. Among the neutral saccharides, contents of galactose, glucose, rhamnose, arabinose, xylose and mannose were detected.

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Sensitive determination of derivatized carbohydrates by high-performance liquid chromatography

Cited by 55 publications

References 18 publications

Pulsed amperometric detection of aliphatic alcohols in liquid chromatography

Pulsed amperometric detection of aliphatic alcohols in liquid chromatography

Extraction and Analysis of Inositols and Other Carbohydrates from Soybean Plant Tissues

Characterization and degradation of pectin derived from Budimka apple

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