Sensitive Detection of Viruses in Pollen Using Conventional and Real‐time Reverse Transcription‐polymerase Chain Reaction

Shiller, Jason; Lebas, B. S. M.; Horner, M.; Pearson, M. N.; Clover, G. R. G.

doi:10.1111/j.1439-0434.2010.01693.x

Cited by 10 publications

(4 citation statements)

References 28 publications

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“…For those virus-or viroidhost combinations, pollinating insects can thereby aid in plant-toplant spread and infection of a healthy plant (20,65). Many countries have strict phytosanitary regulations to ensure that pathogens are not introduced in pollen that is traded internationally as a source of germplasm by breeders (92). Many countries have strict phytosanitary regulations to ensure that pathogens are not introduced in pollen that is traded internationally as a source of germplasm by breeders (92).…”

Section: The Unique Challenges For the Detection And Control Of Systementioning

confidence: 99%

Safeguarding Fruit Crops in the Age of Agricultural Globalization

et al. 2015

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The expansion of fruit production and markets into new geographic areas provides novel opportunities and challenges for the agricultural and marketing industries. Evidence that fruit consumption helps prevent nutrient deficiencies and reduces the risk of cardiovascular disease and cancer has assisted in the expansion of all aspects of the fruit industry. In today's competitive global market environment, producers need access to the best plant material available in terms of genetics and health if they are to maintain a competitive advantage in the market. An ever-increasing amount of plant material in the form of produce, nursery plants, and breeding stock moves vast distances, and this has resulted in an increased risk of pest and disease introductions into new areas. One of the primary concerns of the global fruit industry is a group of systemic pathogens for which there are no effective remedies once plants are infected. These pathogens and diseases require expensive management and control procedures at nurseries and by producers locally and nationally. Here, we review (i) the characteristics of some of these pathogens, (ii) the history and economic consequences of some notable disease epidemics caused by these pathogens, (iii) the changes in agricultural trade that have exacerbated the risk of pathogen introduction, (iv) the path to production of healthy plants through the U.S. National Clean Plant Network and state certification programs, (v) the economic value of clean stock to nurseries and fruit growers in the United States, and (vi) current efforts to develop and harmonize effective nursery certification programs within the United States as well as with global trading partners.

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Section: The Unique Challenges For the Detection And Control Of Systementioning

confidence: 99%

Safeguarding Fruit Crops in the Age of Agricultural Globalization

et al. 2015

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“…From anther dehiscence to release in the atmosphere, airborne pollens come in contact with various atmospheric particles ( Knox et al, 1997 ; Sedghy et al, 2017 ). Airborne pollens constitute a major fraction of bioaerosols and serve as carriers for various pathogens, including viruses ( Jones & Harrison, 2004 ; Shiller, Lebas, Horner, Pearson, & Clover, 2010 ; West, 2014 ). This will affect the prevalence and occurrence of diseases linked to pollen exposure.…”

Section: Airborne Pollen and Virus Interactionmentioning

confidence: 99%

Does airborne pollen influence COVID-19 outbreak?

Ravindra

Goyal

Mor

2021

Sustainable Cities and Society

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The fast spread of SARS-CoV-2 presented a worldwide challenge to public health, economy, and educational system affecting human society’s welfare. With high transmission rates and increasing evidence of COVID-19 spread via bioaerosols. The current review was conducted to examine airborne pollens impact on COVID-19 transmission and to identify the major gaps for post-pandemic research. The study used all key terms to identify revenant literature and observation were collated for the current research. Based on the existing literature, there is a potential association between pollen bioaerosols and COVID-19. There are few studies focusing on the impact of airborne pollen on SARS-CoV-2, which could be useful to advance future research. Allergic rhinitis and asthma patients were found to have pre-modified immune activation, which could help to provide protection against COVID-19. However, does airborne pollen acts as a potent carrier for SARS-CoV-2 transport, dispersal and its proliferation still require further multidisciplinary research. Further, a clear conclusion cannot be drawn due to limited evidence and hence more research is needed to show how pollen bioaerosols could affect virus survivals. The small but growing literature review focuses on searching for every possible answer to provide additional security layers to overcome near future corona-like infectious diseases.

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“…Reverse Transcription Loop-mediated Isothermal Amplification Assay for Detecting Peach Latent Mosaic Viroid in Peach Pollen Because of their unique modes of transmission, these viruses can be stable in infected pollen and, as such, represent a significant risk to phytosanitation even after several years of storage (Shiller et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Reverse Transcription Loop-mediated Isothermal Amplification Assay for Detecting Peach Latent Mosaic Viroid in Peach Pollen

Leehyojeong¹,

Jeong²

2020

HST

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Artificial pollination is used to increase the yield of many fruit crops. However, much of the pollen used for this process is imported from other countries, thereby increasing the risk of introducing plant pathogens, such as peach latent mosaic viroid (PLMVd). According to the Animal and Plant Quarantine Service in Korea, PLMVd is a quarantine pathogen that reduces both fruit yield and tree vigor. Therefore, the aim of the present study was to develop a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for detecting PLMVd in imported pollen. The optimum RT-LAMP conditions were incubation at 63°C for 60 min, and the assay was both highly specific and 10 times more sensitive than RT-PCR. The usefulness of the RT-LAMP assay was also confirmed by screening both imported pollen and leaf samples for PLMVd. Thus, the present study demonstrates the usefulness of RT-LAMP for the rapid screening of PLMVd in field conditions, peach certification programs, and quarantine inspections.

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Sensitive Detection of Viruses in Pollen Using Conventional and Real‐time Reverse Transcription‐polymerase Chain Reaction

Cited by 10 publications

References 28 publications

Safeguarding Fruit Crops in the Age of Agricultural Globalization

Safeguarding Fruit Crops in the Age of Agricultural Globalization

Does airborne pollen influence COVID-19 outbreak?

Reverse Transcription Loop-mediated Isothermal Amplification Assay for Detecting Peach Latent Mosaic Viroid in Peach Pollen

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