Sensitive detection of rare cancer cells in sputum and peripheral blood samples of patients with lung cancer by preproGRP-specific RT-PCR

Lacroix, Jeannine; Becker, Heinrich D.; Woerner, Stefan M.; Rittgen, Werner; Drings, P.; Doeberitz, Magnus von Knebel

doi:10.1002/1097-0215(200102)9999:9999<::aid-ijc1159>3.0.co;2-5

Cited by 47 publications

(21 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The separation and detection of low numbers of bacteria and virus; drug delivery nanoparticles, high molecular weight (hmw) DNA biomarkers, antibodies and other entities from blood and other complex biological samples remains a significant challenge [1][2][3][4][5][6]. For many research and clinical applications, sample preparation is used to enrich a specific analyte before detection is carried out.…”

Section: Introductionmentioning

confidence: 99%

An AC electrokinetic method for enhanced detection of DNA nanoparticles

Krishnan

Heller

2009

Journal of Biophotonics

View full text Add to dashboard Cite

Journal of BIOPHOTONICSIn biomedical research and diagnostics it is a challenge to isolate and detect low levels of nanoparticles and nanoscale biomarkers in blood and other biological samples. While highly sensitive epifluorescent microscope systems are available for ultra low level detection, the isolation of the specific entities from large sample volumes is often the bigger limitation. AC electrokinetic techniques like dielectrophoresis (DEP) offer an attractive mechanism for specifically concentrating nanoparticles into microscopic locations. Unfortunately, DEP requires significant sample dilution thus making the technology unsuitable for biological applications. Using a microelectrode array device, special conditions have been found for the separation of hmw-DNA and nanoparticles under high conductance (ionic strength) conditions. At AC frequencies in the 3000-10 000 Hz range, 10 mm microspheres and human T lymphocytes can be isolated into the DEP low field regions, while hmw-DNA and nanoparticles can be concentrated into microscopic high field regions for subsequent detection using an epifluorescent system.Final separation and detection of 150 ng/mL OliGreen fluorescent stained high molecular weight (hmw) DNA in 1 Â TBE buffer (109 mS/m). The DEP field was applied at 10 000 Hz and 10 volts pk-pk for 5 minutes to group of nine microelectrodes. The green fluorescent image (Ex 505 nm, Em 520 nm) shows fluorescence from the OliGreen stained hmw DNA concentrated on the 80-micron diameter microelectrodes with the far right column of unactivated microelectrodes showing no concentration of hmw-DNA.

show abstract

Section: Introductionmentioning

confidence: 99%

An AC electrokinetic method for enhanced detection of DNA nanoparticles

Krishnan

Heller

2009

Journal of Biophotonics

View full text Add to dashboard Cite

show abstract

“…According to previous studies of lung cancer, CEA was detected in 50% 21 or 60% of patients, 4 cytokeratin 19 in 39% of patients 22 and prepro GRP in 30%. 20 Syndecan 1 (CD 138) is a cell surface transmembrane heparan sulfate proteoglycan. 47 It is located at the surface of many kinds of cells such as fibroblasts and epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…More recently, it has been suggested that the RT-PCR method is more rapid and sensitive. 4,21,29,30 PCR methods have been reported for the detection of CEA, 21 CK19, 31 preprogrowth hormone receptor 20 and MUC1 32 from blood samples of lung cancer patients.…”

mentioning

confidence: 99%

Application of differential display to identify genes for lung cancer detection in peripheral blood

Matsunaga

Hangai

Aso

et al. 2002

Intl Journal of Cancer

View full text Add to dashboard Cite

“…Recently, Lacroix et al (2001) has reported the use of reverse transcription PCR assays for preprogastrinreleasing peptide (preproGRP) to detect rare exfoliated tumour cells in peripheral venous blood and sputum samples in patients with lung cancer. These target transcripts are tightly suppressed in normal nonneoplastic tissues.…”

Section: Sputum Biomarkersmentioning

confidence: 99%