Sensitive Detection of Glycyrrhizin and Evaluation of the Affinity Constants by a Surface Plasmon Resonance-based Immunosensor

Sakai, Takahisa; Shinahara, Katsura; Torimaru, Akira; Tanaka, Hiroyuki; Shoyama, Yukihiro; Matsumoto, Kiyoshi

doi:10.2116/analsci.20.279

Cited by 22 publications

(11 citation statements)

References 22 publications

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“…Although it is well established that changes in refractive index (RI) at surfaces can be used to study molecular interactions (9,10,26,27), some studies (e.g., 28) suggest that molecular interactions can be quantified in free solution by using an intrinsic signal other than temperature, as in ITC. Sota et.al.…”

mentioning

confidence: 99%

Free-Solution, Label-Free Molecular Interactions Studied by Back-Scattering Interferometry

Bornhop

Latham

Kussrow

et al. 2007

Science

190

216

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Free-solution, label-free molecular interactions were investigated with back-scattering interferometry in a simple optical train composed of a helium-neon laser, a microfluidic channel, and a position sensor. Molecular binding interactions between proteins, ions and protein, and small molecules and protein, were determined with high dynamic range dissociation constants (K d spanning six decades) and unmatched sensitivity (picomolar K d 's and detection limits of 10,000s of molecules). With this technique, equilibrium dissociation constants were quantified for protein A and immunoglobulin G, interleukin-2 with its monoclonal antibody, and calmodulin with calcium ion Ca 2+ , a small molecule inhibitor, the protein calcineurin, and the M13 peptide. The high sensitivity of back-scattering interferometry and small volumes of microfluidics allowed the entire calmodulin assay to be performed with 200 picomoles of solute.

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mentioning

confidence: 99%

Free-Solution, Label-Free Molecular Interactions Studied by Back-Scattering Interferometry

Bornhop

Latham

Kussrow

et al. 2007

Science

190

216

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“…For the promotion of effective cultivation of licorice as an additional and stable herbal resource, the rapid and easy measurement methods of the GC content are required. Previously, we established anti-GC MAb and a fast and sensitive assay system using the MAb for the screening of GC concentration in large numbers of samples (Tanaka & Shoyama, 1998;Shan et al, 2001), the sensitive detection of GC by surface plasmon resonance-based immunosensor (Sakai et al, 2004), and the rapid detection by immunochromatographic assay (Putalun et al, 2005). The purpose of our recent studies is to purify and quantify GC from licorice crude extract by anti-GC-MAB immunoaffinity chromatography to expand the possibilities for pharmacological research and other applications.…”

Section: Preparation Of Gc-knock Out Extract and Its Application For mentioning

confidence: 99%

Quality Control by Immunoaffinity Concentration Using Monoclonal Antibody

Uto¹,

Tung²

2011

Quality Control of Herbal Medicines and Related Areas

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“…In this group, researchers most often published data from IAsys (Affinity Sensors ), Spreeta and TISPR-1 (Texas Instruments [978][979][980][981][982][983][984][985][986][987][988][989][990][991][992] , small molecules , and cells, phage and virus particles [866][867][868][869][870][871][872][873][874][875][876][877][878][879][880][881][882][883][884][885]. In the applied sciences, biosensors increasingly contribute in clinical [886][887][888][889][890][891][892][893][894][895][896][897][898][899]…”

Section: Instrumentsmentioning

confidence: 99%

Survey of the year 2004 commercial optical biosensor literature

Rich

Myszka

2005

J of Molecular Recognition

113

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The year 2004 represents a milestone for the biosensor research community: in this year, over 1000 articles were published describing experiments performed using commercially available systems. The 1038 papers we found represent a $ 10% increase over the past year and demonstrate that the implementation of biosensors continues to expand at a healthy pace. We evaluated the data presented in each paper and compiled a 'top 10' list. These 10 articles, which we recommend every biosensor user reads, describe wellperformed kinetic, equilibrium and qualitative/screening studies, provide comparisons between binding parameters obtained from different biosensor users, as well as from biosensor-and solution-based interaction analyses, and summarize the cutting-edge applications of the technology. We also re-iterate some of the experimental pitfalls that lead to sub-optimal data and over-interpreted results. We are hopeful that the biosensor community, by applying the hints we outline, will obtain data on a par with that presented in the 10 spotlighted articles. This will ensure that the scientific community at large can be confident in the data we report from optical biosensors.

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Sensitive Detection of Glycyrrhizin and Evaluation of the Affinity Constants by a Surface Plasmon Resonance-based Immunosensor

Cited by 22 publications

References 22 publications

Free-Solution, Label-Free Molecular Interactions Studied by Back-Scattering Interferometry

Free-Solution, Label-Free Molecular Interactions Studied by Back-Scattering Interferometry

Quality Control by Immunoaffinity Concentration Using Monoclonal Antibody

Survey of the year 2004 commercial optical biosensor literature

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