Sensitive detection of cardiac troponin T based on superparamagnetic bead-labels using a flexible micro-fluxgate sensor

Guo, Lei; Yang, Zhen; Zhi, Shaotao; Zhang, Feng; Lei, Chong; Zhou, Yong

doi:10.1039/c7ra10355g

Cited by 16 publications

(9 citation statements)

References 33 publications

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“…In Figure S6, XPS data of the surface of the Au LSPR strip present the appearance of S and N peaks after MUA and IL-10 antibody were treated, respectively. We observed the increased N signal implying that IL-10 antibody was successfully conjugated with the Au LSPR strip (Guo et al, 2017). Figure 5 shows the extinction spectrum demonstrating the colloidal AuNC-based ELISA on the double-bent Au LSPR strip.…”

Section: Resultsmentioning

confidence: 91%

Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10

et al. 2020

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Localized surface plasmon resonance (LSPR) is a powerful platform for detecting biomolecules including proteins, nucleotides, and vesicles. Here, we report a colloidal gold (Au) nanoparticle-based assay that enhances the LSPR signal of nanoimprinted Au strips. The binding of the colloidal Au nanoparticle on the Au strip causes a red-shift of the LSPR extinction peak, enabling the detection of interleukin-10 (IL-10) cytokine. For LSPR sensor fabrication, we employed a roll-to-roll nanoimprinting process to create nanograting structures on polyethylene terephthalate (PET) film. By the angled deposition of Au on the PET film, we demonstrated a double-bent Au structure with a strong LSPR extinction peak at ∼760 nm. Using the Au LSPR sensor, we developed an enzyme-linked immunosorbent assay (ELISA) protocol by forming a sandwich structure of IL-10 capture antibody/IL-10/IL-10 detection antibody. To enhance the LSPR signal, we introduced colloidal Au nanocube (AuNC) to be cross-linked with IL-10 detection antibody for immunogold assay. Using IL-10 as a model protein, we successfully achieved nanomolar sensitivity. We confirmed that the shift of the extinction peak was improved by 450% due to plasmon coupling between AuNC and Au strip. We expect that the AuNC-assisted LSPR sensor platform can be utilized as a diagnostic tool by providing convenient and fast detection of the LSPR signal.

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Section: Resultsmentioning

confidence: 91%

Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10

et al. 2020

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“…The S and N signal peaks appeared after the MUA and anti-CFH antibody treatment. The IgG antibody has four polypeptide chains and peptides composed of several amino acids; thus, the N signal peaks arise from the anti-CFH antibody immobilized on the AuNM surface . The main provider of S signal peaks was MUA, which consisted of the thiol group and served as a bridge to connect the AuNMs and the antibodies.…”

Section: Resultsmentioning

confidence: 99%

“…38,39 40 thus, the N signal peaks arise from the anti-CFH antibody immobilized on the AuNM surface. 41 The main provider of S signal peaks was MUA, which consisted of the thiol group and served as a bridge to connect the AuNMs and the antibodies. In addition, the AuNM−antibody modification was also examined using a fluorescence microscope.…”

Section: Detection Of Biomarkers With Aunm Chips and Sandwich Immunoa...mentioning

confidence: 99%

Noninvasive Detection of Bladder Cancer Markers Based on Gold Nanomushrooms and Sandwich Immunoassays

Yang

Chang

Chiu

et al. 2023

ACS Appl. Nano Mater.

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Bladder cancer is one of the most common malignancies in the urinary system. Cystoscopy is the traditional standard diagnostic method for bladder cancer with subsequent biopsy or surgery. However, this method is uncomfortable for most patients because it requires anesthesia and possibly causes infections. Because of the high recurrence rate of bladder cancer, a rapid, low-cost, high-sensitivity, and noninvasive sensing method is needed. This study employed gold nanomushroom (AuNM) chips for bladder cancer biomarker detection, combining the benefits of sandwich immunoassay and localized surface plasmon resonance (LSPR) sensing. With a metal nanotransfer printing technique, which is cheap and straightforward, the AuNMs were patterned on flexible polycarbonate (PC) sheets. The gold caps stood above PC stems and provided ample spatial areas for capturing the biomarkers to be sensed. Three biomarkers served as the antigens and analytes, including human complement factor H (CFH), hyaluronic acid (HA), and nuclear matrix protein 22 (NMP22). Different antibodies, against the same biomarker, were covalently conjugated to AuNMs or gold nanoparticles, respectively. When the antibody–antigen–antibody sandwich structure formed, the plasmonic coupling between the AuNM surface and the gold nanoparticles significantly enhanced LSPR signals. The LSPR red shifts correlated quantitatively with the concentrations of the biomarkers. The limits of detection were 6.5, 8.3, and 7.0 pg/mL for CFH, HA, and NMP22, respectively. The chip’s specificity was tested and confirmed, excluding the nonspecific binding and false-positive possibility. The sensing performance of this sandwich immunoassay-based AuNM chip was better than that of the commercialized enzyme-linked immunosorbent assay. It provided a rapid, label-free, and easy operating platform for diagnosing and monitoring bladder cancer.

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“…For application purposes, Guo et al applied the flexible fluxgate sensors for the detection of cardiac troponin T (cTnT), an important biomarker for cardiovascular diseases. 191 In their work, the fluxgate sensor incorporates a double layer of Co-based amorphous ribbons as the sensing element along with three-dimensional solenoid copper coils (one sensing coil and two excitation coils) for sensitive core control, as shown in Figure 9B(a) and 9B(b). The fluxgate sensor was fabricated via standard microfabrication techniques such as photolithography and electroplating.…”

Section: Flexible Fluxgate Sensorsmentioning

confidence: 99%

Flexible Magnetic Field Nanosensors for Wearable Electronics: A Review

Mostufa

Yari

Rezaei

et al. 2023

ACS Appl. Nano Mater.

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Flexible magnetic field nanosensors hold immense potential for wearable electronics, offering a range of advantages such as comfort, real-time health monitoring, motion sensing, durability, and seamless integration with other sensors. They are expected to revolutionize wearable technologies and drive innovation in various domains, enhancing the overall user experience. In this review, we provide an overview of recent advances in flexible magnetic field nanosensors, including flexible Hall sensors, flexible magnetoresistive (MR) sensors such as giant magnetoresistance (GMR), magnetic tunnel junction (MTJ), and anisotropic magnetoresistance (AMR) sensors, flexible fluxgate sensors, and flexible giant magnetoimpedance (GMI) sensors. We discuss different fabrication methods and real-life applications for each type of sensor as well as the technical challenges faced by these sensors. The use of these flexible nanosensors opens more possibilities for human−computer interaction and presents exciting opportunities for wearable technology in diverse fields. The robustness of these sensors along with the trend to reduce energy consumption will continue to be important research areas. Future trends in flexible magnetic field nanosensors include energy harvesting from the body, miniaturization and lower power consumption, improved durability and reliability, and reduced cost. These advancements have the potential to drive the widespread adoption of flexible magnetic field nanosensors in wearable devices, enabling innovative applications and enhancing the overall user experience.

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Sensitive detection of cardiac troponin T based on superparamagnetic bead-labels using a flexible micro-fluxgate sensor

Abstract: In this study, we describe an innovative micro-fluxgate immunosensor based on superparamagnetic Dynabeads for the detection of cardiac troponin T (cTnT), an important biomarker for cardiovascular diseases.

Cited by 16 publications

References 33 publications

Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10

Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10

Noninvasive Detection of Bladder Cancer Markers Based on Gold Nanomushrooms and Sandwich Immunoassays

Flexible Magnetic Field Nanosensors for Wearable Electronics: A Review

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