“…Various analytical techniques have been used to determine 4-NP, including electrochemical sensors, 15 surface enhanced Raman spectroscopy (SERS), 16 high-performance liquid chromatography, 17 mass spectrometry, 18 surface plasmon resonance, 19 capillary electrophoresis, 20 ultraviolet-visible spectrophotometry, 21 fluorescence spectrometry, 22 and indirect enzymatic immunosorbent assay. 23 Among them, SERS has emerged as a powerful nanophotonic technology to detect the spectral signals of analytes even at the single-molecular level owing to its high sensitivity, unique fingerprint characteristics, high accuracy, non-destructiveness, excellent repeatability, rapid acquisition, and multiplexed detection ability.…”