Sensitive colorimetric assays for α-glucosidase activity and inhibitor screening based on unmodified gold nanoparticles

α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

“…The calculated IC 50 values are in good agreement with the values obtained in previously published studies. 6,9 Such findings confirm the feasibility of our approach for α-Glu inhibitor screening.…”

“…Therefore, α-Glu activity detection and its inhibitor screening are crucial, especially for drug discovery. Due to the significance of α-Glu measurement, especially for clinical research, various analytical approaches including electrochemical, colorimetric, , surface plasmon resonance, and fluorometric ,, have been designed for its activity monitoring. Fluorometric and colorimetric approaches are mainly considered among others due to their real-time sensing capability, low cost, and simple operation.…”

Electrochemical assay of α-glucosidase activity and the inhibitor screening in cell medium

“…Moreover, the detection limit has also been calculated to be 0.04 U/mL by the interpolation of the mean plus three times the standard deviation of the zero standards. Though the value is higher than the previous reports with colorimetric method based on the aggregation of AuNPs Chen et al, 2015), the proposed method exhibits strong anti-interference ability. It is well known that the state of AuNPs can be easily affected by various factors such as the amount of salt and pH value of the detection system.…”

A visible and label-free colorimetric sensor for miRNA-21 detection based on peroxidase-like activity of graphene/gold-nanoparticle hybrids

“…Most of the reported colorimetric methods based on the Au-NP-regulated visual strategy are limited by poor stability, because phosphodiester bonds of oligonucleotides are very degradable and Au-NPs tend to agglutinate with changing detection conditions, such as pH, temperature and the concentration of Au-NPs. [22][23][24] Moreover, these colorimetric methods oen require complex probe preparation and modication. Thus, it is urgent to develop new colorimetric platforms with easy design, high response, high stability and easy probe preparation for specic miRNA detections.…”