Sensitive colorimetric assay of hydrogen peroxide and glucose in humoral samples based on the enhanced peroxidase-mimetic activity of NH2-MIL-88-derived FeS2@CN nanocomposites compared to its precursors

Fan, Ru; Tian, Jinrong; Wang, Huili; Wang, Xuedong; Zhou, Peipei

doi:10.1007/s00604-022-05525-w

Cited by 2 publications

(2 citation statements)

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“…The mechanism of peroxidase-like activity of FeS 2 nanoparticles was then further explored. Terephthalic acid was used as a fluorescent probe to detect the production of hydroxyl radicals, which can be produced from H 2 O 2 by peroxidase [ 42 ]. The generation of hydroxyl radicals from H 2 O 2 catalyzed by FeS 2 was detected.…”

Section: Resultsmentioning

confidence: 99%

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Dual-Enzyme Cascade Composed of Chitosan Coated FeS2 Nanozyme and Glucose Oxidase for Sensitive Glucose Detection

Shen¹,

Qing

Zhū

et al. 2023

Molecules

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Immobilizing enzymes with nanozymes to catalyze cascade reactions overcomes many of the shortcomings of biological enzymes in industrial manufacturing. In the study, glucose oxidases were covalently bound to FeS2 nanozymes as immobilization carriers while chitosan encapsulation increased the activity and stability of the immobilized enzymes. The immobilized enzymes exhibited a 10% greater increase in catalytic efficiency than the free enzymes while also being more stable and catalytically active in environments with an alkaline pH of 9.0 and a high temperature of 100 °C. Additionally, the FeS2 nanozyme-driven double-enzyme cascade reaction showed high glucose selectivity, even in the presence of lactose, dopamine, and uric acid, with a limit of detection (LOD) (S/N = 3) as low as 1.9 × 10−6 M. This research demonstrates that nanozymes may be employed as ideal carriers for biological enzymes and that the nanozymes can catalyze cascade reactions together with natural enzymes, offering new insights into interactions between natural and synthetic biosystems.

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Section: Resultsmentioning

confidence: 99%

“…Different concentrations of H 2 O 2 and FeS 2 were mixed in a phosphate buffer with pH = 4.5, and 10 μL of 80 mM TMB solution was then added. The absorbance of the mixture at 652 nm was assessed using a UV-vis spectrophotometer (Perkin Elmer, Waltham, MA, USA) after 5 min of reaction at 37 °C [ 42 ].…”

Section: Methodsmentioning

confidence: 99%