Sensitive biomarker responses of the shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Roles of alpha-tocopherol and metallothioneins

Bertrand, Lidwina; Monferrán, Magdalena V.; Mouneyrac, Catherine; Bonansea, Rocío Inés; Ramón, Adriana Noemí; Amé, María Valeria

doi:10.1016/j.aquatox.2016.08.014

Cited by 49 publications

(21 citation statements)

References 68 publications

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“…In contrast to our findings, Campos et al (47) reported no significant changes in total protein and lipid content in adult D. magna exposed to much higher Flx concentrations (80 µg/L) for 24 h. Instead, they reported increased carbohydrate and oxygen consumption, which may point to higher ROS levels associated with increased aerobic metabolism (29). As several studies suggest higher protein carbonyl levels in oxidative stress (49), which ends with proteasomal degradation of oxidised proteins (50), the lowering of total protein levels in our subacute experiment may display the oxidative damage on tissue proteins.…”

Section: Resultscontrasting

confidence: 99%

Oxidative and apoptotic effects of fluoxetine and its metabolite norfluoxetine in Daphnia magna

Över

Güven

Taşkın

et al. 2020

Archives of Industrial Hygiene and Toxicology

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The aim of this study was to investigate the oxidative and apoptotic potential of fluoxetine, a widely used antidepressant in Turkey and the world, and of its metabolite norfluoxetine on a model non-target organism, Daphnia magna to see how exposure to this group of antidepressants (specific serotonin reuptake inhibitors) could affect the aquatic environment in which they end up. Juvenile D. magna specimens were chronically exposed to fluoxetine and norfluoxetine alone and in combination at concentrations found in the aquatic environment (0.091 and 0.011 μg/L, respectively) and to their 10-fold environmental concentrations for 21 days. Another group of 17-day-old animals were subacutely exposed to 100-fold environmental concentrations for four days. After exposure, we measured their glutathione peroxidase (GPx) and cholinesterase (ChE) activities, thiobarbituric acid-reactive substances (TBARS), and total protein content spectrophotometrically, while mitochondrial membrane potential (MMP) was analysed by fluorescence staining, and cytochrome c and ERK1/2 protein content by Western blotting. This is the first-time cytochrome c and ERK1/2 were determined at the protein level in D. magna. We also measured their carapace length, width, and caudal spine length microscopically. At environmental concentrations fluoxetine and norfluoxetine caused an increase in ChE activity and brood production. They also caused a decrease in juvenile carapace length, width, and caudal spine length and depolarised the mitochondrial membrane. At 10-fold environmental concentrations, GPx activity, lipid peroxidation levels, cytochrome c, and ERK1/2 protein levels rose. The most pronounced effect was observed in D. magna exposed to norfluoxetine. Norfluoxetine also decreased brood production. Similar effects were observed with subacute exposure to 100-fold environmental concentrations. However, total protein content decreased. All this confirms that fluoxetine and norfluoxetine have oxidative and apoptotic potential in D. magna. Daphnia spp. have a great potential to give us precious insight into the mechanisms of environmental toxicants, but there is still a long way to go before they are clarified in these organisms.

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Section: Resultscontrasting

confidence: 99%

Oxidative and apoptotic effects of fluoxetine and its metabolite norfluoxetine in Daphnia magna

Över

Güven

Taşkın

et al. 2020

Archives of Industrial Hygiene and Toxicology

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“…On the other hand, fish biomarkers are useful tools in several steps of the risk assessment process: effect, exposure and hazard assessment, risk characterization or classification, and monitoring the environmental quality of aquatic ecosystems (van der Ooost et al, 2003). However, data provided by this biomarker approach is difficult to interpret without an integrated overview that globally assesses the potential influence of the pollutant under study (Bertrand et al, 2016a). Hence, stress indices also have been developed from this type of parameters.…”

Section: Introductionmentioning

confidence: 99%

“…A prominent example of them is the Integrated Biomarkers Response (IBR) index, which constitutes a practical and robust tool to assess the susceptibility to pollutants using multiple biomarker responses (Beliaeff and Burgeot, 2002;Serafim et al, 2012). Several studies used this index with field data (Damiens et al, 2007;Cravo et al, 2012;Pain-Devin et al, 2014), although it was also utilized as a promising tool to integrate and interpret responses measured in organisms exposed in laboratory experiments (Quintaneiro et al, 2015;Bertrand et al, 2016a).…”

Section: Introductionmentioning

confidence: 99%

An integrated biomarker response study explains more than the sum of the parts: Oxidative stress in the fish Australoheros facetus exposed to imidacloprid

Iturburu

Bertrand

Mendieta

et al. 2018

Ecological Indicators

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Integrated Biomarkers Response (IBR) index have been developed as a practical and robust tool to assess the susceptibility to pollutants using multiple biomarker responses. Neonicotinoid insecticides are nowadays one of the most sold pesticides worldwide. Nevertheless, imidacloprid (IMI) sub-lethal effects such as oxidative stress (OS) on fishes are scarcely studied. Hence, the aims of this work were: (1) to evaluate exposure-and damage biomarkers related to OS in the freshwater fish Australoheros facetus exposed to IMI and (2) to apply the IBR index to achieve a comprehensive understanding of OS in the fish. The results of the present study showed that all the biomarkers presented different responses in the three monitored tissues: liver, brain and gills. Results for an initial battery of 19 biomarkers were obtained and for the IBR index only those with significant differences have been considered. The biomarkers that had the most important weight on the IBR index were SOD activity in brain and gills, H 2 O 2 concentration in liver, and carbonyl groups concentration in gills in fishes exposed to 100 and 1000 μg L −1 IMI. This index allowed affirming that a short term exposure to environmentally relevant concentrations of IMI (≥10 μg L −1) produces OS in A. facetus. However, a more deep understanding of some biomarkers response is necessary to improve the index and for finally apply it in field studies.

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“…An extensive bibliography reports the effects of OP on animals, most of them associated with their consequence on diverse physiological activities such as interference of oxidative metabolism, inhibition of respiratory enzymes and inhibition of esterases (Bertrand et al, 2016b;Bonifacio et al, 2016;Giesy and Solomon, 2014). Chlorpyrifos (CPF), O, O-diethyl-O-(3,5,6-trichloro-2-pyridyl) phosphorothioate is a broad-spectrum OP pesticide extensively used for the control of insects.…”

Section: Introductionmentioning

confidence: 99%

Can a low concentration of an organophosphate insecticide cause negative effects on an aquatic macrophyte? Exposure of Potamogeton pusillus at environmentally relevant chlorpyrifos concentrations

Bertrand

Marino

Monferrán

et al. 2017

Environmental and Experimental Botany

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The contamination of the aquatic environments with organophosphorus pesticides may affect non-target organisms. The aim of this study was to evaluate the toxic effects of the insecticide chlorpyrifos (CPF) at environmental concentrations on the freshwater macrophyte Potamogeton pusillus belonging to a genus of worldwide distribution. For this purpose, individuals were exposed from 3.5 to 94.5 ng of CPF L À1 for 96 h. A battery of biochemical responses including bioaccumulation, defense and damage biomarkers were measured in leaf, stem and root. Even when CPF was not detected in the macrophyte tissues, our results showed that this insecticide promotes oxidative stress and biomolecule damages in P. pusillus after acute exposure. Significant response of biomarkers was observed from the lowest tested concentration (3.5 ng CPF L À1). Oxidative stress was evidenced by increased lipid peroxidation and antioxidant enzymatic activation, including changes in superoxide dismutase, guaiacol peroxidase and glutathione peroxidase activities, especially in leaf. Also, a significant decrease in chlorophyll a and b contents was observed mainly in leaf. Finally, with some selected biomarkers, an Integrated Biomarker Response index was calculated showing a dose-response relationship with CPF exposure. Previous studies reported that herbicides and organophosphorus pesticides are responsible for several effects on photosynthetic systems but at higher exposure concentrations than the tested in this study. These results draw attention to the need for more studies in toxic effects of insecticides on aquatic macrophytes, at low concentrations and different biological levels, since the protection guidelines would not be preserving these species.

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Sensitive biomarker responses of the shrimp Palaemonetes argentinus exposed to chlorpyrifos at environmental concentrations: Roles of alpha-tocopherol and metallothioneins

Cited by 49 publications

References 68 publications

Oxidative and apoptotic effects of fluoxetine and its metabolite norfluoxetine in Daphnia magna

Oxidative and apoptotic effects of fluoxetine and its metabolite norfluoxetine in Daphnia magna

An integrated biomarker response study explains more than the sum of the parts: Oxidative stress in the fish Australoheros facetus exposed to imidacloprid

Can a low concentration of an organophosphate insecticide cause negative effects on an aquatic macrophyte? Exposure of Potamogeton pusillus at environmentally relevant chlorpyrifos concentrations

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