Sensitive and specific gas chromatographic and extraction method for the determination of orphenadrine in human body fluids

Labout, J. J. M.; Thussen, C.T.; Hespe, W.

doi:10.1016/s0021-9673(00)99355-5

Cited by 10 publications

(1 citation statement)

References 3 publications

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“…Gas chromatographic methods have been developed for the quantitative determination of orphenadrine in human serum, plasma, and urine (Labout et al, 1977;Lutz et al, 1983;Contin et al, 1988;Bilzer and Gundert-Remy, 1973). The related substances test for orphinadrine citrated in British pharmacopoeia, 2007(Righetti, 1996 also relies on GC.…”

Section: Introductionmentioning

confidence: 99%

Development of a capillary electrophoresis method for the determination of orphenadrine citrate in tablets in the presence of paracetamol

Haj-Ali

Hamdan

2010

Saudi Pharmaceutical Journal

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A validated method using capillary electrophoresis was developed, for the determination of orphenadrine citrate in its tablet formulations, in the presence of paracetamol. The method employs a running buffer of 30 mM pentane sulfonate sodium, dissolved in 20 mM MOPS buffer pH 7.7. Samples were injected using hydrodynamic sample injection mode (25 mbar, for 25 s), using positive polarity of 25 kV, at a constant temperature of 30 °C. Samples of orphenadrine citrate alone or in mixture solutions with paracetamol were exposed to various degradation conditions, and were electrophoresed using the recommended condition. The method was found to be specific, linear (r (2) = 0.994), precise, accurate, and robust, with an LOQ of 0.02 mg/mL. The proposed method was successfully applied for measurement of the percentage per label of orphenadrine citrate in commercially available tablets.

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Section: Introductionmentioning

confidence: 99%

Development of a capillary electrophoresis method for the determination of orphenadrine citrate in tablets in the presence of paracetamol

Haj-Ali

Hamdan

2010

Saudi Pharmaceutical Journal

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Simple and rapid GLC method for the determination of orphenadrine in human plasma

Contin

Riva

Albani

et al. 1987

Biomedical Chromatography

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A rapid and specific method has been developed for the determination of orphenadrine concentration in plasma. It involves a one-step sample preparation using n-hexane/isopropyl alcohol (98:2) extraction, and analysis by gas chromatography on a wide bore capillary column using nitrogen/phosphorus detection. This procedure considerably simplifies previously reported assays and is specific and sensitive enough for the determination of orphenadrine in plasma of patients on chronic therapy.

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Difference between single and multiple dose pharmacokinetics of orphenadrine hydrochloride in man

Labout

Thijssen

Keijser

et al. 1982

Eur J Clin Pharmacol

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Plasma concentrations of orphenadrine were measured by a specific gas chromatographic method in 5 healthy male volunteers after a single oral dose of orphenadrine hydrochloride 100mg. The single dose pharmacokinetic profile of orphenadrine was evaluated from these data. The elimination half-life ranged from 13.2-20.1h after the commercial tablet formulation. Plasma concentrations, determined in volunteers and patients under different conditions of repeated oral administration of the same formulation of orphenadrine hydrochloride exceeded the theoretical values, predicted from the single dose pharmacokinetics, by a factor 2 to 3. The elimination half-lives after discontinuation of treatment showed a 2 to 3-fold increase over the single dose values. This demonstrates a clear discrepancy between the multiple and single dose pharmacokinetics of orphenadrine. Experiments in dogs suggested competition for biotransformation between orphenadrine and its metabolite N-demethylorphenadrine. Product inhibition of this type could explain the observed discrepancy.

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Sensitive and specific gas chromatographic and extraction method for the determination of orphenadrine in human body fluids

Cited by 10 publications

References 3 publications

Development of a capillary electrophoresis method for the determination of orphenadrine citrate in tablets in the presence of paracetamol

Development of a capillary electrophoresis method for the determination of orphenadrine citrate in tablets in the presence of paracetamol

Simple and rapid GLC method for the determination of orphenadrine in human plasma

Difference between single and multiple dose pharmacokinetics of orphenadrine hydrochloride in man

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