Sensitive and Specific Detection of
            <i>Yersinia pseudotuberculosis</i>
            by Loop-Mediated Isothermal Amplification

Horisaka, Tomoko; Fujita, Kayoko; Iwata, Tadahisa; Nakadai, Aya; Okatani, Alexandre Tomomitsu; Horikita, Tetsuya; Taniguchi, Takahide; Honda, Eiichi; Yokomizo, Yuichi; Hayashidani, Hideki

doi:10.1128/jcm.42.11.5349-5352.2004

Cited by 62 publications

(35 citation statements)

References 16 publications

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“…Heat-treated C. albicans cell suspensions prepared directly from growth on plates showed a detection limit for the LAMP reaction consistently below 10 cells in the reaction mixture (Fig. 3C), in good agreement with the results of other authors who followed the same experimental approach using whole bacterial cells (13,15). A different electrophoretic banding pattern was occasionally (Ͻ1%) detected in LAMP assays (Fig.…”

Section: Vol 46 2008 Identification Of Clinically Relevant Yeast Spsupporting

confidence: 80%

“…The performances of LAMP-and PCR-based diagnostic systems, including real-time technologies (23,40), have been extensively compared. In general, LAMP was found to be either similar or superior to PCR, and more specific (e.g., 8,13,15), but a few studies proved otherwise, such as those reported by Kato et al (18), who showed that although LAMP was 10-fold more sensitive than standard PCR for the detection of the Clostridium difficile toxin B gene (tcdB), an optimized nested-PCR assay performed much better than LAMP. In our experience, an optimization step can be critical for improving the sensitivity of both LAMP-and PCR-based methods.…”

Section: Discussionmentioning

confidence: 99%

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Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes

2008

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The occurrence of invasive mycoses has progressively increased in recent years. Yeasts of the genus Candida remain the leading etiologic agents of those infections. Early identification of opportunistic yeasts may contribute significantly to improved disease management and the selection of appropriate antifungal therapy. We developed a rapid and reliable molecular identification system for clinically relevant yeasts that makes use of nonspecific primers to amplify a region of the 26S rRNA gene, followed by reverse hybridization of the digoxigenin-labeled products to a panel of species-specific oligonucleotide probes arranged on a nylon membrane macroarray format. DNA amplification was achieved by the recently developed loop-mediated isothermal DNA amplification technology, a promising option for the development of improved laboratory diagnostic kits. The newly developed method was successful in distinguishing among the major clinically relevant yeasts associated with bloodstream infections by using simple, rapid, and cost-effective procedures and equipment.

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Section: Vol 46 2008 Identification Of Clinically Relevant Yeast Spsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes

2008

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“…The LAMP assay is a novel method to amplify DNA under isothermal conditions (20,21) and has been applied to the identification or detection of some bacteria with high sensitivity and specificity (8,10). The method is more rapid and easier to perform than a PCR assay and does not require any special equipment, such as a thermal cycler or electrophoresis system.…”

Section: Discussionmentioning

confidence: 99%

Rapid and Simple Method for Detecting the Toxin B Gene of Clostridium difficile in Stool Specimens by Loop-Mediated Isothermal Amplification

Kato

Yokoyama²,

Kato

et al. 2005

J Clin Microbiol

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We applied the loop-mediated isothermal amplification (LAMP) assay to the detection of the toxin B gene (tcdB) of Clostridium difficile for identification of toxin B (TcdB)-positive C. difficile strains and detection of tcdB in stool specimens. tcdB was detected in all toxin A (TcdA)-positive, TcdB-positive (A ؉ B ؉ ) and TcdA-negative, TcdB-positive (A ؊ B ؉ ) C. difficile strains but not from TcdA-negative, TcdB-negative strains. Of the 74 stool specimens examined, A ؉ B ؉ or A ؊ B ؉ C. difficile was recovered from 39 specimens, of which 38 specimens were LAMP positive and one was negative. Amplification was obtained in 10 specimens that were culture negative, indicating that LAMP is highly sensitive. The LAMP assay was applied to detection of tcdB in DNA extracted by a simple boiling method from 47 of those 74 specimens, which were cultured overnight in cooked-meat medium (CMM). Twenty-two of 24 culture-positive specimens were positive for LAMP on DNA from the culture in CMM. Four specimens were culture negative but positive by LAMP on DNA from CMM cultures. The LAMP assay is a reliable tool for identification of TcdB-positive C. difficile as well as for direct detection of tcdB in stool specimens with high sensitivity. Detection of tcdB by LAMP from overnight cultures in CMM could be an alternative method of diagnostic testing at clinical laboratories without special apparatus.

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“…Two outbreaks causing more than 500 infections were reported in China in the 1980s (24). Yersinia enterocolitica has a broad animal reservoir, with swine being the most common source of infection for humans (10,11,13,18). Y. enterocolitica is distributed among a diverse range of animals in China.…”

mentioning

confidence: 99%

Pathogenic Strains ofYersinia enterocoliticaIsolated from Domestic Dogs (Canis familiaris) Belonging to Farmers Are of the Same Subtype as PathogenicY. enterocoliticaStrains Isolated from Humans and May Be a Source of Human Infection in Jiangsu Province, China

et al. 2010

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We isolated 326 Yersinia enterocolitica strains from 5,919 specimens from patients with diarrhea at outpatient clinics, livestock, poultry, wild animals, insect vectors, food, and the environment in the cities of Nantong and Xuzhou in Jiangsu Province, China, from 2004 to 2008. The results showed that the 12 pathogenic strains were of the O:3 serotype. Six strains were isolated from domestic dogs (Canis familiaris) belonging to farmers and were found to be the primary carriers of pathogenic Y. enterocolitica strains, especially in Xuzhou. Pulsed-field gel electrophoresis analysis of the pathogenic strains from dogs belonging to farmers showed that they shared the same patterns as strains from diarrhea patients isolated in 1994. This indicates that the strains from domestic dogs have a close correlation with the strains causing human infections.

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Sensitive and Specific Detection of Yersinia pseudotuberculosis by Loop-Mediated Isothermal Amplification

Cited by 62 publications

References 16 publications

Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes

Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes

Rapid and Simple Method for Detecting the Toxin B Gene of Clostridium difficile in Stool Specimens by Loop-Mediated Isothermal Amplification

Pathogenic Strains ofYersinia enterocoliticaIsolated from Domestic Dogs (Canis familiaris) Belonging to Farmers Are of the Same Subtype as PathogenicY. enterocoliticaStrains Isolated from Humans and May Be a Source of Human Infection in Jiangsu Province, China

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