Sensitive and Specific Detecting Biomarker of Radiation-Resistant Nasopharyngeal Carcinoma by TpTta-COF Nanosheet

Abstract

An important attribute of microRNA-205 (MiR-205) is their potential use as predictive biomarker for anti-radiation of nasopharyngeal carcinoma (NPC), and it is pivotal to monitor the dynamic change of MiR-205 for personalized precise treatment strategies. So far, there are several methods aiming at detecting MiR-205 while rarely has worked out questions such as limited complex detection processes, poor sample detection limit or spending lots of time. Therefore, a method for detecting MiR-205 based on 2D COF nanosheets and fluorescent oligonucleotide probe was constructed and investigated in this study. The 2D COF nanosheet quenches the fluorescence of the fluorescent single-stranded DNA (ss-DNA) probes through fluorescence resonance energy transfer (FRET). The method enables to capture MiR-205 sensitively in aqueous solution with a detection limit of 4.78[Formula: see text]nM in the range 0–500[Formula: see text]nM and [Formula: see text], and the method offers great specificity in that it can distinguish the target miRNA from mismatch nontarget miRNAs. Considering its simple operability and excellent specificity, it has great application prospects in the detection of miRNA biomarker in clinical diagnosis and personalized treatment plan.

Ratiometric Fluorescent Biosensor Based on Self-Assembled Fluorescent Gold Nanoparticles and Duplex-Specific Nuclease-Assisted Signal Amplification for Sensitive Detection of Exosomal miRNA

“…These novel strategies are based on various functional nanomaterials and different principles such as fluorescence, surface-enhanced Raman spectroscopy, colorimetry, surface plasmon resonance, electrochemistry, photoelectrochemistry, and electrochemiluminescence. − Fluorescence-based methods are most commonly used for the determination of miRNAs due to their high sensitivity and simple operation . Various nanomaterials with fluorescence emission or quenching functions such as gold nanoparticles (Au NPs), Ti 3 C 2 T x , MoS 2 , g-C 3 N 4 , black phosphorus, graphene oxide, MnO 2 nanosheets, magnetic nanoparticles, metal-organic frameworks, and covalent organic frameworks have been explored for the detection of miRNAs. − Among them, Au NPs can effectively quench the fluorescence of fluorescent molecules through an energy transfer phenomenon. For instance, Zhao et al constructed a molecular beacon by attaching Cy5-modified hairpin probes to Au NP-coated polystyrene microbeads .…”