Sensitive and simultaneous detection of multi-index lung cancer biomarkers by an NIR-Ⅱ fluorescence lateral-flow immunoassay platform

Ao, Lijiao; Liao, Tao; Huang, Liang; Lin, Shan; Xu, Kui; Ma, Jiangtao; Qiu, Shaorong; Wang, Xiangyu; Zhang, Qiqing

doi:10.1016/j.cej.2022.135204

Cited by 53 publications

(26 citation statements)

References 52 publications

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“…The limit of detection is determined to be 0.01 ng/mL (S/N = 3). The CEA detecting performance of this CL immunosensor is equal to and/or even better than the commercialized kits (Table S3) as well as some recently reported immunosensors. − …”

Section: Resultsmentioning

confidence: 65%

A General Route for Chemiluminescence of n-Type Au Nanocrystals

Zhang

Gao

et al. 2022

Anal. Chem.

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The photoluminescence, electroluminescence, and electrochemiluminescence from nanocrystals (NCs) have been extensively exploited for both fundamental and applied investigation over two decades, while the understanding of chemiluminescence (CL) from NCs is still far from clear by now. Herein, a general route for triggering CL from NC luminophore is proposed by extensively exploiting the charge transfer between n-type NCs and oxidants. Oxidants, such as K2S2O8, H2O2, KMnO4, and NaClO, can chemically inject the hole onto the valence band (VB) of methionine-capped n-type AuNCs (Met@AuNCs) and enable the occurrence of efficient radiative-charge-recombination between the chemically injected exogenous VB hole and the pre-existed endogenous conduction band (CB) electron, which eventually results in single-color and defect-involved CL with the maximum emission wavelength around 824 nm. The CL of Met@AuNCs/oxidant is qualified for ultrasensitive CL immunoassay in a similar procedure to the biotin–avidin and magnetic separation involved commercial CL immunoassay and exhibits acceptable performance for linearly determining carcinoembryonic antigen from 50 pg/mL to 100 ng/mL with a limit of detection of 10 pg/mL (S/N = 3). This strategy provides a general route to develop nanoparticulate CL luminophores and might eventually enable CL multiplexing assay via extensively exploiting the CL of different wavebands.

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Section: Resultsmentioning

confidence: 65%

A General Route for Chemiluminescence of n-Type Au Nanocrystals

Zhang

Gao

et al. 2022

Anal. Chem.

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“…However, the traditional colloidal gold nanoparticles (AuNPs)-based LFIA has poor quantitative ability, high false negative rates and limited sensitive, due to the absence of readout system and the single signal of colorimetric [ [22] , [23] , [24] ]. With the development of nanomaterials, many novel materials, such as quantum dots (QDs) [ 25 , 26 ], up-conversion luminescence materials [ 27 ], fluorescent microsphere [ 28 ], and magnetic nanobeads (MBs) [ 29 ] are used as labels in LFIA and greatly promote the development of LFIA and realizes the quantitative detection of LFIA. Due to the strong photostability, high quantum yield, and tunable fluorescent wavelength of QDs with a signal readout system for LFIA, the QDs-based LFIA could offer high sensitivity and quantitative analysis property [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Magnetic/fluorescent dual-modal lateral flow immunoassay based on multifunctional nanobeads for rapid and accurate SARS-CoV-2 nucleocapsid protein detection

Xie

Feng²,

Pei³

et al. 2022

Analytica Chimica Acta

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“…Most conventional test strips for tumor biomarker detection are based on the double-antibody sandwich principle. − However, the usage of double antibodies undoubtedly raises the cost, and the activity of antibodies needs to be considered for the overall design and assembly of the test strip, making the development of test strips more difficult. Since the pregnancy test strip has been successfully commercialized, its cost has been greatly reduced due to its widespread application.…”

Section: Introductionmentioning

confidence: 99%

Detection of Matrix Metalloproteinase-1 in Human Saliva Based on a Pregnancy Test Strip Platform

et al. 2022

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Matrix metalloproteinase (MMP) is closely correlated with tumorigenesis and progression. Establishing a low-cost, simple, rapid, and sensitive method for its detection is highly desired for the broad-spectrum screening of oral cancer. Herein, we combine the MMP-specific cleavage ability with magnetic separation technology and a commercial test strip to construct a sensitive biosensor to detect MMP-1 conveniently for the first time. The method involves two DNA probes, peptide–DNA1 and hCG–DNA2, where DNA1 and DNA2 are complementary sequences, and the peptide labeled with biotin can bind streptavidin-modified magnetic nanoparticles stably. The human chorionic gonadotropin (hCG) is the target of the pregnancy test strip. The cleavage reaction mediated by MMP-1 releases peptide–DNA1 and the hybridized hCG–DNA2 into the solution, and the hCG probe in the solution can develop color on the test strip for the determination of MMP-1 after magnetic separation. This method utilizes the high specificity of MMP-1’s proteolytic cleavage and the high sensitivity of the test strip to the target probe, achieving a sensitive detection of MMP-1 with a visual detection limit of 65.5 pg/mL. The method shows better anti-interference and sensitivity than the enzyme-linked immunosorbent assay in the application of a biological sample matrix, suggesting its great potential for clinical diagnosis, especially for broad-spectrum oral cancer screening.

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Sensitive and simultaneous detection of multi-index lung cancer biomarkers by an NIR-Ⅱ fluorescence lateral-flow immunoassay platform

Cited by 53 publications

References 52 publications

A General Route for Chemiluminescence of n-Type Au Nanocrystals

A General Route for Chemiluminescence of n-Type Au Nanocrystals

Magnetic/fluorescent dual-modal lateral flow immunoassay based on multifunctional nanobeads for rapid and accurate SARS-CoV-2 nucleocapsid protein detection

Detection of Matrix Metalloproteinase-1 in Human Saliva Based on a Pregnancy Test Strip Platform

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