Sensitive and Selective Whole Cell Detection of Escherichia Coli from Waters by a Paper-Based Immunosensor with Laser-Induced Fluorescence Detection

Wang, Wei; Yu, Xiufeng; Zhang, Huimin; Zhang, Shaoyan

doi:10.2139/ssrn.3950174

Cited by 1 publication

(2 citation statements)

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“…Research has been conducted on bacterial detection for food and environmental control using various methods for different applications [187][188][189]. S. aureus [143,190,191], S. typhimurium [144,192,193], Microcystin-LR [145,194], E. coli [195][196][197], L. monocytogenes [198,199] and P. aeruginosa [200][201][202] are popular pathogens that can be fatal to humans and animals. Bhardwaj et al [143] developed a labelfree µPAD EI for the detection of S. aureus using an antibody-(Ab-) single-walled carbon nanotube (SWCNT) bio-conjugate with DPV.…”

Section: µPed Immunosensors For Bacteriamentioning

confidence: 99%

“…Research has been conducted on bacterial detection for food and environmental control using various methods for different applications [ 187 – 189 ]. S. aureus [ 143 , 190 , 191 ], S. typhimurium [ 144 , 192 , 193 ], Microcystin-LR [ 145 , 194 ], E. coli [ 195 – 197 ], L. monocytogenes [ 198 , 199 ] and P. aeruginosa [ 200 – 202 ] are popular pathogens that can be fatal to humans and animals. Bhardwaj et al .…”

Section: Paper-based Eismentioning

confidence: 99%

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Advances in paper-based electrochemical immunosensors: review of fabrication strategies and biomedical applications

du Plooy,

Jahed,

Iwuoha

et al. 2023

R. Soc. open sci.

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Cellulose paper-based sensing devices have shown promise in addressing the accuracy, sensitivity, selectivity, analysis time and cost of current disease diagnostic tools owing to their excellent physical and physiochemical properties, high surface-area-to-volume ratio, strong adsorption capabilities, ease of chemical functionalization for immobilization, biodegradability, biocompatibility and liquid transport by simple capillary action. This review provides a comprehensive overview of recent advancements in the field of electrochemical immunosensing for various diseases, particularly in underdeveloped regions and globally. It highlights the significant progress in fabrication techniques, fluid control, signal transduction and paper substrates, shedding light on their respective advantages and disadvantages. The primary objective of this review article is to compile recent advances in the field of electrochemical immunosensing for the early detection of diseases prevalent in underdeveloped regions and globally, including cancer biomarkers, bacteria, proteins and viruses. Herein, the critical need for new, simplistic early detection strategies to combat future disease outbreaks and prevent global pandemics is addressed. Moreover, recent advancements in fabrication techniques, including lithography, printing and electrodeposition as well as device orientation, substrate type and electrode modification, have highlighted their potential for enhancing sensitivity and accuracy.

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