Sensitive and selective determination of GSH based on the ECL quenching of Ru(II) 1,10-phenanthroline-5,6-dione complex

Xu, Yanxue; Zhang, Lei; Liu, Yuan; Jin, Zhaoyu; Zhao, Qian; Yang, Feng; Xiao, Dingquan

doi:10.1016/j.bios.2015.09.033

Cited by 23 publications

(8 citation statements)

References 36 publications

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“…The development of new reaction sites and new mechanisms for thiol uorescent probes will constantly be a key research area in the following years. 91,92 Biological insight promoted by thiol probes Utilizing these known thiol uorescent probe design strategies, we can obtain the desired tools through rational molecular design. Fluorescent probes that have a specic response toward one of the biothiols allow us to evaluate concentration changes under different conditions without the potential interference caused by other species.…”

Section: Evolution Of Fluorescent Probes For Thiolsmentioning

confidence: 99%

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The chronological evolution of small organic molecular fluorescent probes for thiols

2021

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Section: Evolution Of Fluorescent Probes For Thiolsmentioning

confidence: 99%

“…The development of new reaction sites and new mechanisms for thiol fluorescent probes will constantly be a key research area in the following years. 91,92 …”

Section: Evolution Of Fluorescent Probes For Thiolsmentioning

confidence: 99%

The chronological evolution of small organic molecular fluorescent probes for thiols

2021

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“…The ECL intensity of 1 gradually decreased with addition of Cys as expected. To demonstrate the stability of 1 in human serum, chronoamperometry (CA) , coupled ECL experiments were conducted by a 40 cycle continuous scan, and the initial ECL intensity remained almost unchanged (Figure b), indicating that sensor 1 was stable. These results suggest that sensor 1 could potentially be applied to ECL clinical diagnosis.…”

Section: Resultsmentioning

confidence: 99%

Photoluminescence and Electrochemiluminescence Dual-Signaling Sensors for Selective Detection of Cysteine Based on Iridium(III) Complexes

Kim

Hong

2019

ACS Omega

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Cysteine (Cys) is important in biosynthesis, detoxification, and metabolism. The selective detection of Cys over structurally similar homocysteine (Hcy) or glutathione (GSH) remains an immense challenge. Although there are many methods for detecting Cys, photoluminescence (PL) and electrochemiluminescence (ECL) techniques are well-suited for clinical diagnostics and analytical technology because of their high sensitivities. Herein, we report PL and ECL dual-channel sensors using cyclometalated iridium(III) complexes for the discrimination of Cys from Hcy and GSH. The sensors react with cysteine preferentially because of kinetic differences in intramolecular conjugate addition/cyclization, enabling phosphorescence enhancement and ECL decrease in the blue-shifted region. Sensor 1 shows ratiometric PL turn-on and ECL turn-off for Cys. In addition, unique ECL-enhancing behavior of sensor 1 toward GSH enables discrimination between Cys and GSH. Sensor 1 was successfully applied to the detection of Cys in human serum by the ECL method. We demonstrate the first case of a Cys-selective PL and ECL dual-channel chemodosimetric sensor based on cyclometalated iridium(III) complexes and expect that the rational design of efficient PL and ECL dual-channel sensors will be useful in diagnostic technology.

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“…However, there were a variety of metabolic disorders which leaded to altered Tyr degradation and organic aciduria in humans, including tyrosinemia, phenylketoneuria and severe liver disease . Due to the biological and clinical significance of GSH and Tyr, researches concerning their individual analysis have been widely conducted . Up till now, no attention has been paid to their simultaneous determination by electrochemical method.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric Separation and Determination of Glutathione and L‐tyrosine with Chlorogenic Acid as an Electrocatalytic Mediator

Zhang

Chen

et al. 2017

Electroanalysis

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A novel and sensitive voltammetric method was proposed for separation and determination of glutathione (GSH) and L‐tyrosine (Tyr) at acetylene black and chitosan modified glassy carbon electrode (AB‐CS/GCE). By introducing chlorogenic acid (CGA) as a new electrocatalytic mediator, GSH could be detected at much lower potential with symmetric peak shape. Acetylene black and chitosan composite served as current signal amplifier for sensitive detection. The electrochemical behavior of GSH and Tyr in the presence of CGA was studied at AB‐CS/GCE and complete separation of anodic peaks was achieved. Under the optimum conditions, the electrocatalytic oxidation peak current of GSH showed a linear dependence on its concentration in the ranges of 2.0×10−7‐4.0×10−5 M with the detection limit of 5.8×10−8 M (S/N=3), while the oxidation peak current of Tyr was linear to its concentration from 2.5×10−6 to 4.3×10−4 M with the detection limit of 9.2×10−7 M (S/N=3) by differential pulse voltammetry (DPV). The established method has been applied to the simultaneous determination of GSH and Tyr in human urine with satisfactory results.

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Sensitive and selective determination of GSH based on the ECL quenching of Ru(II) 1,10-phenanthroline-5,6-dione complex

Cited by 23 publications

References 36 publications

The chronological evolution of small organic molecular fluorescent probes for thiols

The chronological evolution of small organic molecular fluorescent probes for thiols

Photoluminescence and Electrochemiluminescence Dual-Signaling Sensors for Selective Detection of Cysteine Based on Iridium(III) Complexes

Voltammetric Separation and Determination of Glutathione and L‐tyrosine with Chlorogenic Acid as an Electrocatalytic Mediator

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