Sensitive and selective colorimetric assay of alkaline phosphatase activity with Cu(II)-phenanthroline complex

Hu, Qiong; He, Minhui; Mei, Yaqi; Feng, Wen-Jie; Jing, Su; Kong, Jinming; Zhang, Xueji

doi:10.1016/j.talanta.2016.10.097

Cited by 54 publications

(13 citation statements)

References 51 publications

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“…The concentration of ALP that could be visualized by the naked eye was as low as 0.01 U/L. Compared with the recent developed colorimetric methods for ALP detection are listed in Table S1 (Supporting Information), the proposed method is more sensitive and simpler. ,,− …”

Section: Results and Discussionmentioning

confidence: 99%

Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay

et al. 2018

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Herein, a redox-cycling was proposed to amplify the signal of enzyme-linked immunosorbent assay (ELISA), which was performed in a polystyrene microplate based on a classic sandwich-type. After the sandwich immunoreactions were finished, the alkaline phosphatase captured on a microplate triggered the hydrolyzation of L-ascorbic acid 2-phosphate to generate ascorbic a c i d ( A A ) , w h i c h t h e n r e d u c e d c o l o r l e s s t r i s -(bathophenanthroline) iron(III) (Fe(BPT) 3 3+) encapsulated in the micelle of TX-100 to pink red tris(bathophenanthroline) iron(II) (Fe(BPT) 3 2+ ). In the presence of tris(2-carboxyethyl)phosphine, the oxidation product, dehydroascorbic acid, was transformed to AA quickly which then reduced Fe(BPT) 3 3+ again and again, resulting in the generation of abundant Fe(BPT) 3 2+ that could be read out conveniently by a commercial microplate reader or the naked eye. Because the negative charged TCEP with large size could hardly pass through the micelle, the reduction of Fe(BPT) 3 3+ by TCEP directly was negligible. Experiment results for assay of alpha-fetoprotein (a model antigen) showed the cycling greatly improved the detection limit to 5 pg/mL, 2 orders of magnitude lower than that of conventional ELISA. The cycling also exhibited the advantages of simplicity and high reproducibility, implying its great potential for practical applications in biological and clinical diagnosis.

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Section: Results and Discussionmentioning

confidence: 99%

Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay

et al. 2018

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“…The absorbance was measured at 546 nm, and AST levels were expressed as U/L. ALP activity was measured following p ‐nitrophenyl phosphate conversion to a yellow substrate when dephosphorylated [28]. The absorbance was measured at 405 nm, and the ALP levels were expressed as U/L.…”

Section: Methodsmentioning

confidence: 99%

Calcium dobesilate protects against d‐galactose‐induced hepatic and renal dysfunction, oxidative stress, and pathological damage

Hakimizadeh

Heidari

Giménez‐Llort

et al. 2022

Fundamemntal Clinical Pharma

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Calcium dobesilate (CaD) is used for the treatment of diabetic retinopathy and nephropathy. This agent exerts antioxidant effects. In the present study, we evaluated the protective effects of oral administration of CaD against hepatorenal damages in a mice model of aging induced by D-galactose (Dgal). We used 28 male albino mice, which equally and randomly were divided into four groups as follows: intact, aging (D-gal at the dose of 500 mg/kg, p.o.), aging + CaD 50 (D-gal plus CaD at the dose of 50 mg/kg), and aging + CaD 100 (D-gal plus CaD at the dose of 100 mg/kg, p.o.). All drugs were administered orally once a day for 42 days. The liver and kidney damages were evaluated by measuring mass indices, levels of serum creatinine and blood urea nitrogen, and activities of serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase and by histopathological evaluation. Moreover, hepatic and renal tissue oxidant/ antioxidant markers (malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase) were measured. The results showed that D-gal treatment induced significant oxidative stress in the kidney and liver that was paralleled by dysfunctions and histological alterations of these organs. CaD significantly improved the liver and kidney indices, implemented functional capacity of the liver and kidney, as well as decreased oxidative stress

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“…It is likely that novel probes will improve colorimetric assays. Hu et al (2017) proposed a practical and simple method for the selective and sensitive colorimetric assay of ALP activity [85]. The researchers used a water soluble and biologically conducive Cu(II)-phenanthroline complex as a probe.…”

Section: Optical Detection Techniquesmentioning

confidence: 99%

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

Balbaied

Moore

2019

Biosensors

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Alkaline phosphatase (ALP), which catalyzes the dephosphorylation process of proteins, nucleic acids, and small molecules, can be found in a variety of tissues (intestine, liver, bone, kidney, and placenta) of almost all living organisms. This enzyme has been extensively used as a biomarker in enzyme immunoassays and molecular biology. ALP is also one of the most commonly assayed enzymes in routine clinical practice. Due to its close relation to a variety of pathological processes, ALP’s abnormal level is an important diagnostic biomarker of many human diseases, such as liver dysfunction, bone diseases, kidney acute injury, and cancer. Therefore, the development of convenient and reliable assay methods for monitoring ALP activity/level is extremely important and valuable, not only for clinical diagnoses but also in the area of biomedical research. This paper comprehensively reviews the strategies of optical and electrochemical detection of ALP and discusses the electrochemical techniques that have been addressed to make them suitable for ALP analysis in cell culture.

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Sensitive and selective colorimetric assay of alkaline phosphatase activity with Cu(II)-phenanthroline complex

Cited by 54 publications

References 51 publications

Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay

Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay

Calcium dobesilate protects against d‐galactose‐induced hepatic and renal dysfunction, oxidative stress, and pathological damage

Overview of Optical and Electrochemical Alkaline Phosphatase (ALP) Biosensors: Recent Approaches in Cells Culture Techniques

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