Sensitive and rapid detection of Aeromonas caviae in stool samples by loop-mediated isothermal amplification

Wei, Xiao; Zheng, Ze; Zhang, Li Huan; Qu, Fengli; Huang, Xi‐Tai

doi:10.1016/j.diagmicrobio.2007.07.021

Cited by 9 publications

(9 citation statements)

References 14 publications

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“…The PCR products were analyzed by submarine gel electrophoresis in 1% agarose gel and visualized by UV after staining with ethidium bromide. For comparison, 16S-23S ITS amplifications by PCR using the primers F3 and B3 were also performed as described in a previous report (Wei et al 2008).…”

Section: Methodsmentioning

confidence: 99%

“…In our preliminary study, some verified A. caviae isolates did not yield PCR-positive reactions specific to 16S-23S ITS region using primers as described by Wei et al (2008). Therefore, in this study new sets of PCR primers targeting the gyrB gene were developed.…”

mentioning

confidence: 99%

“…For example, A. caviae and A. trota have only a single nucleotide difference (Martinez-Murcia et al 1992) and A. aquariorum and A. caviae are 99.98% identical (Figueras et al 2011). Recently, loop-mediated isothermal amplification (LAMP) based on a 16S-23S internal transcribed spacer (ITS) for A. caviae detection was developed, but only clinical isolates were tested (using primers as described by Wei et al 2008). The housekeeping genes including gyrB, rpoD, rpoB, dnaJ, recA, and cpn60 were described for the identification of Aeromonas spp.…”

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confidence: 99%

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Development of a PCR Assay Based on a Single–Base Pair Substitution for the Detection of Aeromonas caviae by Targeting the gyrB Gene

et al. 2015

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Aeromonas caviae is a bacterial pathogen that causes various infectious diseases in both humans and animals. To facilitate its detection, we developed species-specific primer sets targeting polymorphisms in the gyrB gene for use in a PCR assay. The technique was able to detect 100% (29/29) of the A. caviae strains tested using either of two sets of primers (designated ACF1-ACR and ACF3-ACR), which produced 293-bp and 206-bp amplicons, respectively. Another set of primers (designated ACF2-ACR) yielded a 237-bp amplicon and exhibited 90% (26/29) positive results with respect to A. caviae. None of the primer sets exhibited cross-reactivity with 12 non-A. caviae isolates and 52 other non-Aeromonas bacteria. The detection limit using the ACF2-ACR and ACF3-ACR primer sets in pure culture was 1.6 × 10(3) CFU/mL, or 6 CFU per reaction, whereas that of the ACF1-ACR primer set was 1.6 × 10(4) CFU/mL, or 60 CFU per reaction. In the case of spiked Nile Tilapia Oreochromis niloticus, the sensitivity of all primer sets without enrichment was 1.8 × 10(4) CFU/g, or 30 CFU per reaction. Primer set ACF3-ACR was the best for a PCR assay targeting the gyrB gene, and the PCR technique developed was rapid, specific, and sensitive for the identification of A. caviae.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Development of a PCR Assay Based on a Single–Base Pair Substitution for the Detection of Aeromonas caviae by Targeting the gyrB Gene

et al. 2015

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“…Recently, derivative LAMP assays, such as reverse-transcription LAMP assay [57], multiplex LAMP assay [58], in situ LAMP assay [59], and real-time reverse-transcription LAMP assay [60] have been developed and employed for the detection of various foodborne pathogens, including porcine parvo virus [61], Campylobacter jejuni and Campylobacter coli [62], Vibrio parahaemolyticus [63], Aeromonas caviae [64], Escherichia coli [55], Staphylococcus aureus [65], etc.…”

Section: Loop-mediated Isothermal Amplification (Lamp)mentioning

confidence: 99%

Molecular methods for the detection and characterization of foodborne pathogens

Shi

Long

Suo

2010

Pure and Applied Chemistry

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The surveillance of foodborne pathogens in food industries has shown the urgent need for rapid and dependable methods to detect and characterize the organisms in food and environments of clinical and epidemiologic importance. Recent studies on rapid methods in microbiology have been focused on biochemical characterization, immunoassays, and molecular methods. Many molecular methods have been developed and applied to the detection and characterization of foodborne pathogens in laboratories and food industries. They can be mainly divided into DNA banding pattern-based tests and DNA sequence-based tests. The former includes nucleic acid hybridization, polymerase chain reaction (PCR), amplified restriction length polymorphism, and randomly amplified polymorphic DNA, etc. Most of these methods in commercial applications are based on PCR or hybridization techniques. The principle, characteristics, and application of molecular methods for the detection and characterization of foodborne pathogens were reviewed in this article.

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“…Os casos mais severos ocorreram em indivíduos com fatores predisponentes; no entanto, em indivíduos saudáveis, na maioria dos casos, a evolução da doença é auto-limitada (PEREIRA et al, 2004 e GHATAK et al, 2007. Em 85% dos casos clínicos em que as Aeromonas foram consideradas como principal agente, as espécies A.hydrophila, A. caviae e A. veronii biovar sobria foram identificadas com maior freqüência (CHIM et al, 2007;WEI et al, 2008).…”

Section: Ambiente Contagem Típica (Ufc/ml)*unclassified

Análise do perfil de resistência a antibióticos e detecção dos genes de virulência e resistência em Aeromonas provenientes de amostras ambientais

Moura¹

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Sensitive and rapid detection of Aeromonas caviae in stool samples by loop-mediated isothermal amplification

Cited by 9 publications

References 14 publications

Development of a PCR Assay Based on a Single–Base Pair Substitution for the Detection of Aeromonas caviae by Targeting the gyrB Gene

Development of a PCR Assay Based on a Single–Base Pair Substitution for the Detection of Aeromonas caviae by Targeting the gyrB Gene

Molecular methods for the detection and characterization of foodborne pathogens

Análise do perfil de resistência a antibióticos e detecção dos genes de virulência e resistência em Aeromonas provenientes de amostras ambientais

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