Sensitive and quantitative detection of cardiac troponin I with upconverting nanoparticle lateral flow test with minimized interference

Abstract

Measurement of cardiac troponin I (cTnI) should be feasible for point-of-care testing (POCT) to diagnose acute myocardial infarction (AMI). Lateral flow immunoassays (LFIAs) have been long implemented in POCT and clinical settings. However, sensitivity, matrix effect and quantitation in lateral flow immunoassays (LFIAs) have been major limiting factors. The performance of LFIAs can be improved with upconverting nanoparticle (UCNP) reporters. Here we report a new methodological approach to quantify cTnI using UCNP-LFIA technology with minimized plasma interference. The performance of the developed UCNP-LFIA was evaluated using clinical plasma samples (n = 262). The developed UCNP-LFIA was compared to two reference assays, the Siemens Advia Centaur assay and an in-house well-based cTnI assay. By introducing an anti-IgM scrub line and dried EDTA in the LFIA strip, the detection of cTnI in plasma samples was fully recovered. The UCNP-LFIA was able to quantify cTnI concentrations in patient samples within the range of 30–10,000 ng/L. The LoB and LoD of the UCNP-LFIA were 8.4 ng/L and 30 ng/L. The method comparisons showed good correlation (Spearman’s correlation 0.956 and 0.949, p < 0.0001). The developed UCNP-LFIA had LoD suitable for ruling in AMI in patients with elevated cTnI levels and was able to quantify cTnI concentrations in patient samples. The technology has potential to provide simple and rapid assay for POCT in ED setting

Chemiluminescence Biosensor for the Determination of Cardiac Troponin I (cTnI)

“…The cTnI epitope of the monoclonal mouse antibody clone 9707 was obtained from the corresponding data sheet of the supplier Medix Biochemica [17] and as published previously [18]. Based on this information, the following peptide amino acid sequence (Pep188-199) with a molecular weight of 1548.1 g/mol was chosen and synthesized by peptide & elephants GmbH: NH2-CGGGDWRKNIDALSG-NH2.…”

Latest Trends in Lateral Flow Immunoassay (LFIA) Detection Labels and Conjugation Process

“…The reaction mixture was incubated with stirring for 30 min at room temperature, and the reaction was quenched by the addition of a 50 mM glycine solution. After a further 30 min incubation step, the nanoparticles were washed by centrifugation twice and a solution of 25 mM borate pH 7.8, 150 mM NaCl, 0.1% NaN3, 2 mM KF, 0.2% BSA was added to the pellet ( Bayoumy et al, 2021 ).…”

Review on cardiovascular disease and antihypertensive drugs effect on the circulating biomarkers of heart disease

“…This is used as a detection factor for myocardial infarction. It is also implicated in cardiac muscle damage and injury [41]. Cardiac troponin is specifically important in myocardial infarction because of its high sensitivity and specificity in myocardial injury.…”