Sensitive and Highly Selective Biosensor Based on Triangular Au Nanoplates for Detection of Uric Acid in Human Serum

Wang, Xue; Li, Zhonggui; Lai, Juanhua; Tang, Xiaomin; Qiu, Ping

doi:10.1007/s42250-018-0001-0

Cited by 13 publications

(3 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is unable to undergo further oxidation to 5-hydroxyisourate because of the lack of urate oxidase in humans [ 1 ]. Under normal physiological conditions, the concentration of UA ranges from 120 to 460 μM in blood serum and 1.4 to 4.4 mM in urinary excretion [ 2 , 3 ]. Maintaining a normal level of UA in bodily fluids is vital for ensuring proper health because changes in the concentration of UA can cause or reflect a host of diseases.…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

Rui

Yan

et al. 2021

Microchim Acta

Self Cite

View full text Add to dashboard Cite

Graphical abstract A simple dual-read assay for uric acid (UA) was developed based on a combined ratiometric fluorescent and colorimetric strategy using nitrogen-doped carbon dots (N-CDs). The biosensor relies on the oxidation of UA by uricase to produce H 2 O 2 , which was then converted to • OH radicals by I - , resulting in the oxidation of o -phenylenediamine (OPD) to 2,3-diaminophenazine (DAP). In the presence of UA, the colorless biosensor system changed to yellow. Furthermore, the presence of DAP quenched the fluorescence emission of the N-CDs at 427 nm based on the inner filter effect (IFE). With increasing UA concentrations, the fluorescence intensity of the biosensor at 427 nm decreased but increased at 580 nm, demonstrating the ratiometric response. A strong linearity was observed between the fluorescence intensity ratio of DAP to N-CDs ( I 580 / I 427 ) and the corresponding UA concentration over the range 0.5−150 μM, and a limit of detection (S/N ratio of 3) of 0.06 μM was calculated. The dual-read assay was successfully employed in the quantitation of UA in human serum and urine samples, revealing its potential for measuring UA in clinical samples. Supplementary Information The online version contains supplementary material available at 10.1007/s00604-021-04971-2.

show abstract

Section: Introductionmentioning

confidence: 99%

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

Rui

Yan

et al. 2021

Microchim Acta

Self Cite

View full text Add to dashboard Cite

show abstract

“…The range of UA in human serum is 120–460 μM. 2 Serious conditions such as Lesch Nyhan syndrome, renal disorders, and gout can be brought on by abnormally high levels of UA. 3 High UA levels, frequently referred to as hyperuricemia, are linked to hypertension, cardiovascular and cerebrovascular disorders, and metabolic syndrome.…”

Section: Introductionmentioning

confidence: 99%

Uric acid quantification via colorimetric detection utilizing silver oxide-modified activated carbon nanoparticles functionalized with ionic liquid

Nishan,

Ahmed,

Muhammad

et al. 2024

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…AuNPs possess good conductivity, high biocompatibility and they can easily immobilize the thiolated bio-elements by the strong Au-S bond [22]. Furthermore, there are a large application of gold nanomaterial in different field [23][24][25]. In other hand, carbon black is amorphous nanomaterial, offers a high conductivity and has been considered recently as the most cost-effective modifier for improving the analytical performances of sensors [26].…”

Section: Introductionmentioning

confidence: 99%

A Highly Sensitive Electrochemical Biosensor Based on Carbon Black and Gold Nanoparticles Modified Pencil Graphite Electrode for microRNA-21 Detection

Yammouri

Mohammadi

2019

Chemistry Africa

View full text Add to dashboard Cite

This work aims to develop cost-effective, simple and sensitive electrochemical biosensor for detection of microRNA-21. The combination of carbon black (CB) and gold nanoparticles (AuNPs) as nanohybrid was employed for the first time as a platform for microRNA-21 biosensor fabrication. The developed biosensor is based on the immobilization of thiolated capture probe (complementary sequence of microRNA-21) labeled with methylene blue on the surface of the pencil graphite electrode modified with CB/AuNPs nanohybrid. After hybridization with the target microRNA-21, the orientation of the labeled capture probe changed which causes a decrease of the response of methylene blue oxidation. Differential pulse voltammetry was used for monitoring the methylene blue response before and after hybridization. Under the optimal conditions, the developed biosensor was characterized using differential pulse voltammetry (DPV), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The detection of microRNA-21 was carried out using a DPV. A wide linear range was obtained between 2.9 fM and 0.7 µM of microRNA-21. The calculated limit of detection was 1 fM of microRNA-21. This approach shows a good reproducibility, stability and an excellent selectivity. The proposed biosensor was used for microRNA-21 analysis in serum and a satisfactory result was obtained.

show abstract

Sensitive and Highly Selective Biosensor Based on Triangular Au Nanoplates for Detection of Uric Acid in Human Serum

Cited by 13 publications

References 38 publications

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

A highly sensitive dual-read assay using nitrogen-doped carbon dots for the quantitation of uric acid in human serum and urine samples

Uric acid quantification via colorimetric detection utilizing silver oxide-modified activated carbon nanoparticles functionalized with ionic liquid

A Highly Sensitive Electrochemical Biosensor Based on Carbon Black and Gold Nanoparticles Modified Pencil Graphite Electrode for microRNA-21 Detection

Contact Info

Product

Resources

About