Sensitive Analysis of Sialic Acid and Related Compound by Hydrophilic Interaction Liquid Chromatography Using Fluorescence Detection after Derivatization with DBD-PZ

Abstract

N-Acetylneuraminic acid (NANA) has been reported to react with hydrogen peroxide in vitro to produce 4-(acetylamino)-2,4-dideoxy-D-glycero-D-galacto-octonic acid (ADOA). We labeled NANA and ADOA with 4-(N,N-dimethylaminosulfonyl)-7-piperazino-2,1,3-benzoxadiazole (DBD-PZ) for simultaneous detection. The derivatized NANA and ADOA were separated using hydrophilic interaction liquid chromatography (HILIC) with fluorescence detection. The calibration curves of DBD-PZ-derivatized NANA and ADOA showed good linearity in the range of 221 fmol to 1.5 nmol, and 44 fmol to 1.5 nmol, respectively. This analytical method has high specificity and is useful for the detection of NANA and ADOA in saliva and serum.

Assays for the identification and quantification of sialic acids: Challenges, opportunities and future perspectives

“…The method is very specific and is also highly sensitive with studies reporting detection limits of sialic acids as low as 12 pg to as high as 0.46 ng. 76,78,80,81 One study reported the lower limit of detection as 0.3 mg but this appears to be due to the fact that this was the lowest size standard sample used by the authors and it appears that further validation with smaller sample quantities could be carried out. 79 DMB labelling has one disadvantage in that the derivatized sialic acids are not very stable for periods over 24 hours; they are only stable for hours at room temperature 82 and are sensitive to UV light.…”

Highly Sensitive Fluorescence Detection of Daptomycin in Murine Samples through Derivatization with 2,3-Naphthalenedialdehyde

“…A fluorescence derivatization method is generally popular as well as UV detection, and the sensitivity is comparable to MS detection. 16 In this study, we developed a highly sensitive quantification method using fluorescent derivatization of DAP using 2,3-naphthalenedialdehyde (NDA).…”

Long-wavelength Fluorogenic Derivatization of Aryl Halides Based on the Formation of Stilbene by Heck Reaction with Vinylbenzenes

“…Until now, various fluorescence derivatization reagents for typical functional groups such as amine, phenol, and carbonyl groups have been developed. [1][2][3][4][5][6][7][8][9] However, fluorescence derivatization can be difficult if the target molecules do not have typical functional groups that can be derivatized. Among such compounds that are difficult to derivatize, our research group have focused on aryl halides.…”