Sensitive analysis of recombination activity using integrated cell surface reporter substrates

Christine, Rainer; Siebenkotten, Gregor; Radbruch, Andreas

doi:10.1002/(sici)1097-0320(19991101)37:3<205::aid-cyto7>3.0.co;2-9

Cited by 3 publications

(1 citation statement)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To understand the cis elements that are required for class-switch recombination, various in vitro assay systems were developed using artificial DNA constructs and B cells or B-cell lines [30][31][32][33][34][35][36] . One of the most effective was a system using an efficiently switching mouse lymphoma cell line (CH12F3-2), in which 80% of the cells switch from expressing IgM to (almost exclusively) IgA in one week after stimulation with CD40L, IL-4 and TRANSFORMING GROWTH FACTOR β (TGF-β) 37 .…”

Section: Recognition and Cleavage Of Target Dnamentioning

confidence: 99%

Linking class-switch recombination with somatic hypermutation

Kinoshita

Honjo

2001

Nat Rev Mol Cell Biol

139

112

View full text Add to dashboard Cite

The recent discovery of a molecular link between two apparently different genetic alteration events--class-switch recombination and somatic hypermutation--has led to the idea that the recognition and cleavage of target DNA in these two events might be mediated by similar or identical molecules to those involved in RNA editing. This could mean that the complexity of mammalian genetic information may be enriched by an interplay between RNA editing and DNA modification.

show abstract

Section: Recognition and Cleavage Of Target Dnamentioning

confidence: 99%

Linking class-switch recombination with somatic hypermutation

Kinoshita

Honjo

2001

Nat Rev Mol Cell Biol

139

112

View full text Add to dashboard Cite

show abstract

Efficiency of Iε promoter-directed switch recombination in GFP expression-based switch constructs works synergistically with other promoter and/or enhancer elements but is not tightlylinked to the strength of transcription

Zhang

Yamada

et al. 2002

Eur. J. Immunol.

View full text Add to dashboard Cite

One key unresolved issue in immunoglobulin class switch recombination (CSR) is how the accessibility of the switch region for CSR is controlled. To better understand the nature of accessibility control for human Ig CSR, we developed a novel inducible switch recombination assay based on expression of green fluorescence protein (GFP) from switch constructs undergoing substrate switch recombination (SSR). Efficient SSR depends on the cytokine‐inducible Iϵ promoter and co‐stimulation with IL‐4+anti‐CD40. Characterization of SSR reveals that both S‐S deletional recombination and S‐Sinversion occur. We show that the IL‐4‐inducible Iϵ promoter (pIϵ) selectively determines the efficiency of the accessibility for SSR. However, the pIϵ‐induced transcription, by itself,is not sufficient to direct efficient SSR. For efficient SSR, both pIϵ‐driven transcriptional activity and an additional promoter/enhancer‐derived activity are required. The efficiency of SSR is not tightly correlated with the strength of the combined transcriptional activity. Our results suggest that the mechanism(s) underlying the transcriptional activity, e.g. DNA modification is important for controlling the accessibility for efficient switch recombination.

show abstract