Sensititre® MYCOTB MIC plate for drug susceptibility testing of &lt;I&gt;Mycobacterium tuberculosis&lt;/I&gt; complex isolates

Ma, Yao; Gl, Jiang; St, Chen; Gr, Wang; Hr, Huang

doi:10.5588/ijtld.15.0573

Cited by 19 publications

(17 citation statements)

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“…However, the number of strains resistant to Cs in the present and other three studies were all small. In the present study, sensitivity for EMB at 5μg/ml was 100% (95%CI, 90.8–100.0), similar with 98.2% in Laslie’s study, but significantly higher than that (64.3%, 95%CI (55.4–73.2)) in study of Jongseok et al [ 11 ] and that in Yu’s study[19]. Some studies showed that phenotypic DST method for ethambutol is not as accurate as other drugs such as RFP or INH, with low inter-laboratory agreement[20,21].…”

Section: Discussionsupporting

confidence: 84%

“…The 95% confidence interval for Cs and EMB at 10μg/ml in the present study overlap those in study of Jongseok et al Another study conducted by Laslie et al[18] reported that the sensitivities of Cs, EMB at 10ug/ml were both 100%. One study conducted in China by Yu showed that the sensitivities for Cs and EMB were 100% and 64.6%, respectively[19]. However, the number of strains resistant to Cs in the present and other three studies were all small.…”

Section: Discussioncontrasting

confidence: 47%

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Assessment of a 96-Well Plate Assay of Quantitative Drug Susceptibility Testing for Mycobacterium Tuberculosis Complex in China

et al. 2017

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ObjectiveTo evaluate the performance of the Sensitire MYCOTB MIC Plate (MYCOTB) which could measure the twelve anti-tuberculosis drugs susceptibility on one 96-wells plate.MethodsA total of 140 MDR-TB strains and 60 non-MDR strains were sub-cultured and 193 strains were finally tested for drug resistance using MYCOTB and agar proportion method (APM) and another 7 strains failed of subculture. The drugs included ofloxacin (Ofx), moxifloxacin (Mfx), rifampin (RFP), amikacin (Am), rifabutin (Rfb), para-aminosalicylic acid (PAS), ethionamide (Eth), isoniazid (INH), kanamycin (Km), ethambutol (EMB), streptomycin (Sm), and cycloserine(Cs). The categorical agreement, conditional agreement, sensitivity and specificity of MYCOTB were assessed in comparison with APM. For strains with inconsistent results between MYCOTB and APM, the drug resistance related gene fragments were amplified and sequenced: gyrA for Ofx and Mfx; rpoB for RFP and Rfb; embB for EMB; rpsl for Sm; katG and the promoter region of inhA for INH, ethA and the promoter region of inhA for Eth. The sequence results were compared with results of MYCOTB and APM to analyze the consistency between sequence results and MYCOTB or APM.ResultsThe categorical agreement between two methods for each drug ranged from 88.6% to 100%. It was the lowest for INH (88.6%). The sensitivity and specificity of MYCOTB ranged from 71.4% to 100% and 84.3% to 100%, respectively. The sensitivity was lowest for Cs(71.4%), EMB at 10μg/ml (80.0%) and INH at 10.0μg/ml (84.6%). The specificity was lowest for Rfb (84.3%). Overall discordance between the two phenotypic methods was observed for 96 strains, of which 63 (65.6%) were found susceptible with APM and resistant with MYCOTB and the remaining 33(34.4%) strains were resistant by APM and susceptible with MYCOTB. 34/52 (65.4%) sequenced APM susceptible and MYCOTB resistant(APM-S/MYCOTB-R) strains had mutations or insertions in the amplified regions. 20/30 (66.7%) sequenced APM resistant and MYCOTB susceptible strains had mutations in the sequenced genes. MICs of twenty-nine of these thirty isolates were equal to or within 1 doubling dilution of the critical concentration.ConclusionMYCOTB had good performance for most of tested drugs and could be used as an alternative to the more labor demanding and longer turnaround time solid culture based DST method for detection of drug susceptibility in China.

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Section: Discussionsupporting

confidence: 84%

Section: Discussioncontrasting

confidence: 47%

Assessment of a 96-Well Plate Assay of Quantitative Drug Susceptibility Testing for Mycobacterium Tuberculosis Complex in China

et al. 2017

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“…For most of the drugs, resistant and susceptible strains had clearly different MIC distributions on the UKMYC5 plate (except for linezolid, which showed no resistant strains; clofazimine, para -aminosalicylic acid, and ethionamide were not tested). We did not observe the lack of accuracy for ethambutol observed in previous studies using the MYCOTB plate (17, 21). Since APM and MGIT generally classified the same strains as resistant, similar distributions were observed when MGIT was used to classify the strains (Fig.…”

Section: Resultscontrasting

confidence: 99%

“…Broth microdilution methods, including several using colorimetric indicators, have previously been developed that assess the MICs of a panel of compounds by using a single microtiter plate (5, 10, 11). A dry-format, 96-well microtiter plate assay (the Sensititre MYCOTB plate; Thermo Fisher Scientific Inc., USA) containing 12 drugs has been commercially available since 2010, and early validation studies have returned promising results (12–17). At present, however, neither clinical breakpoints nor epidemiological cutoffs have been defined for any microtiter plate, nor have any plate-based assays included both new and repurposed drugs that will be key to the successful treatment of individual MDR-TB cases in the future.…”

Section: Introductionmentioning

confidence: 99%

Validating a 14-Drug Microtiter Plate Containing Bedaquiline and Delamanid for Large-Scale Research Susceptibility Testing of Mycobacterium tuberculosis

Rancoita

Cugnata

et al. 2018

Antimicrob Agents Chemother

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The UKMYC5 plate is a 96-well microtiter plate designed by the CRyPTIC Consortium (Comprehensive Resistance Prediction for Tuberculosis: an International Consortium) to enable the measurement of MICs of 14 different antituberculosis (anti-TB) compounds for >30,000 clinical Mycobacterium tuberculosis isolates. Unlike the MYCOTB plate, on which the UKMYC5 plate is based, the UKMYC5 plate includes two new (bedaquiline and delamanid) and two repurposed (clofazimine and linezolid) compounds.

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“…Broth microdilution methods, including using several colorimetric indicators, have been developed and used as research tools or to establish the ECOFF for new drugs . A dry‐format, 96‐well microtitre plate assay (Sensititre MYCOTB plate; Thermo Fisher Scientific Inc., Waltham, MA, USA) has been commercially available since 2010 and early validation studies have returned promising results . This assay performs MIC for 12 anti‐TB drugs on the same plates.…”

Section: Phenotypic Dstmentioning

confidence: 99%

Drug resistance mechanisms and drug susceptibility testing for tuberculosis

et al. 2018

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Tuberculosis (TB) caused by Mycobacterium tuberculosis (MTB) is the deadliest infectious disease and the associated global threat has worsened with the emergence of drug resistance, in particular multidrug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB). Although the World Health Organization (WHO) End-TB Strategy advocates for universal access to antimicrobial susceptibility testing, this is not widely available and/or it is still underused. The majority of drug resistance in clinical MTB strains is attributed to chromosomal mutations. Resistance-related mutations could also exert certain fitness cost to the drug-resistant MTB strains and growth fitness could be restored by the presence of compensatory mutations. Understanding these underlying mechanisms could provide an important insight into TB pathogenesis and predict the future trend of MDR-TB global pandemic. This review covers the mechanisms of resistance in MTB and provides a comprehensive overview of current phenotypic and molecular approaches for drug susceptibility testing, with particular attention to the methods endorsed and recommended by the WHO.

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Sensititre® MYCOTB MIC plate for drug susceptibility testing of <I>Mycobacterium tuberculosis</I> complex isolates

Abstract: MYCOTB is a rapid, convenient, quantitative and accurate method for testing both first- and second-line anti-tuberculosis drugs.

Cited by 19 publications

References 0 publications

Assessment of a 96-Well Plate Assay of Quantitative Drug Susceptibility Testing for Mycobacterium Tuberculosis Complex in China

Assessment of a 96-Well Plate Assay of Quantitative Drug Susceptibility Testing for Mycobacterium Tuberculosis Complex in China

Validating a 14-Drug Microtiter Plate Containing Bedaquiline and Delamanid for Large-Scale Research Susceptibility Testing of Mycobacterium tuberculosis

Drug resistance mechanisms and drug susceptibility testing for tuberculosis

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