Sensing platform for nucleic-acid detection based on a 2-aminopurine probe sheared by trans-cleavage activity of the CRISPR/Cas12a system

Abstract

Target double-stranded DNA (dsDNA) or single-stranded DNA (ssDNA) can activate the trans-cleavage activity of the CRISPR/Cas12a, cutting the surrounding non-target ssDNA arbitrarily. In typical CRISPR/Cas12a system, this non-target ssDNA with...

2-Aminopurine-based quencher-free DNA tweezers with fluorescence properties well tuned by surrounding bases

“…The uorescence of 2-AP is highly quenched by base-stacking interaction with natural bases in DNA oligonucleotides, making it an extraordinarily sensitive uorescent probe for nucleic acid structure and biosensors. [22][23][24] Several mechanisms have been proposed for the quenching effect of 2-AP, mainly focusing on photoinduced electron transfer with neighboring bases. [25][26][27][28] The phenomenon of 2-AP quenching has been used to study mispair recognition, base ipping, local melting, protein binding, and electron and energy transfer.…”