Sensing of proteins in human serum using conjugates of nanoparticles and green fluorescent protein

De, Mrinmoy; Rana, Subinoy; Akpinar, Handan; Miranda, Oscar R.; Arvizo, Rochelle R.; Bunz, Uwe H. F.; Rotello, Vincent M.

doi:10.1038/nchem.334

Cited by 441 publications

(348 citation statements)

References 28 publications

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“…The use of monoclonal antibodies can also be useful for detection of small quantities of proteins. Recently, fluorescence based sensing methods using nanoparticles have been developed for more sensitive and cost effective evaluation of proteins levels in the serum [51,52].…”

Section: Biophotonicsmentioning

confidence: 99%

Improved protocols for vibrational spectroscopic analysis of body fluids

Bonnier¹,

Petitjean

Baker

et al. 2013

Journal of Biophotonics

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The applications of vibrational spectroscopy to the examination of human blood serum are explored. Although FTIR spectra can be recorded in aqueous solutions at (gelatin) concentrations as low as 100 mg/L, the high-wavenumber region remains obscured by water absorption. Using Raman spectroscopy, high quality spectra of gelatine solutions as low as 10 mg/L can be achieved, also covering the high-wavenumber regions. In human serum, spectral profiles are weak and partially obscured by water features. Dried deposits are shown to be physically and chemically inhomogeneous resulting in reduced measurement reproducibility. Concentration of the serum using commercially available centrifugal filter devices results in an improvement in the spectral intensity and quality. Additionally, in Raman spectroscopy, reduced background and significantly enhanced signal collection is achievable by measurement in an inverted geometry. The improved protocols for spectroscopic measurement of human serum are applicable to a range of bodily fluids and should accelerate potential clinical applications

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Section: Biophotonicsmentioning

confidence: 99%

Improved protocols for vibrational spectroscopic analysis of body fluids

Bonnier¹,

Petitjean

Baker

et al. 2013

Journal of Biophotonics

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“…Fluorescence-based techniques for protein sensing applications have gained an ever increasing popularity due to their simplicity and exquisite sensitivity (De et al, 2009;Ibraheem and Campbell, 2010;You et al, 2007). In particular, quantum dots (QDs), also called semiconductor nanoparticles, are emerging as a new class of fluorescent probes (Boeneman et al, 2009;Larson et al, 2003;Pinaud et al, 2006).…”

Section: Quantum Dotsmentioning

confidence: 99%

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Kobeissy

Gülbakan

Alawieh

et al. 2014

OMICS: A Journal of Integrative Biology

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The post-genomics era has brought about new Omics biotechnologies, such as proteomics and metabolomics, as well as their novel applications to personal genomics and the quantified self. These advances are now also catalyzing other and newer post-genomics innovations, leading to convergences between Omics and nanotechnology. In this work, we systematically contextualize and exemplify an emerging strand of post-genomics life sciences, namely, nanoproteomics and its applications in health and integrative biological systems. Nanotechnology has been utilized as a complementary component to revolutionize proteomics through different kinds of nanotechnology applications, including nanoporous structures, functionalized nanoparticles, quantum dots, and polymeric nanostructures. Those applications, though still in their infancy, have led to several highly sensitive diagnostics and new methods of drug delivery and targeted therapy for clinical use. The present article differs from previous analyses of nanoproteomics in that it offers an in-depth and comparative evaluation of the attendant biotechnology portfolio and their applications as seen through the lens of post-genomics life sciences and biomedicine. These include: (1) immunosensors for inflammatory, pathogenic, and autoimmune markers for infectious and autoimmune diseases, (2) amplified immunoassays for detection of cancer biomarkers, and (3) methods for targeted therapy and automatically adjusted drug delivery such as in experimental stroke and brain injury studies. As nanoproteomics becomes available both to the clinician at the bedside and the citizens who are increasingly interested in access to novel post-genomics diagnostics through initiatives such as the quantified self, we anticipate further breakthroughs in personalized and targeted medicine.

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“…Our hypothesis was that if a cross-reactive array could be created that was responsive to the subtle structural differences inherent in glycerides, it could be used to pattern individual glycerides, identify structural features of unknown glycerides, and potentially quantitate glycerides in a mixture. Cross-reactive arrays have been successfully used in a number of sensing applications (28)(29)(30)(31)(32)(33). Differential sensing mimics the mammalian senses of olfaction and gustation by detecting the pattern of response of an analyte to a collection of semiselective receptors (34,35).…”

Section: Significancementioning

confidence: 99%

Differential sensing for the regio- and stereoselective identification and quantitation of glycerides

Diehl

Ivy

Rabidoux

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Glycerides are of interest to the areas of food science and medicine because they are the main component of fat. From a chemical sensing perspective, glycerides are challenging analytes because they are structurally similar to one another and lack diversity in terms of functional groups. Furthermore, because animal and plant fat consists of a number of stereo-and regioisomeric acylglycerols, their components remain challenging analytes for chromatographic and mass spectrometric determination, particularly the quantitation of species in mixtures. In this study, we demonstrated the use of an array of cross-reactive serum albumins and fluorescent indicators with chemometric analysis to differentiate a panel of mono-, di-, and triglycerides. Due to the difficulties in identifying the regio-and stereochemistry of the unsaturated glycerides, a sample pretreatment consisting of olefin cross-metathesis with an allyl fluorescein species was used before array analysis. Using this simple assay, we successfully discriminated 20 glycerides via principal component analysis and linear discriminant analysis (PCA and LDA, respectively), including stereo-and regioisomeric pairs. The resulting chemometric patterns were used as a training space for which the structural characteristics of unknown glycerides were identified. In addition, by using our array to perform a standard addition analysis on a mixture of triglycerides and using a method introduced herein, we demonstrated the ability to quantitate glyceride components in a mixture.array sensing | glyceride | chemometrics | serum albumin | differential sensing

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Sensing of proteins in human serum using conjugates of nanoparticles and green fluorescent protein

Cited by 441 publications

References 28 publications

Improved protocols for vibrational spectroscopic analysis of body fluids

Improved protocols for vibrational spectroscopic analysis of body fluids

Post-Genomics Nanotechnology Is Gaining Momentum: Nanoproteomics and Applications in Life Sciences

Differential sensing for the regio- and stereoselective identification and quantitation of glycerides

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