Sensing for intracellular thiols by water-insoluble two-photon fluorescent probe incorporating nanogel

Aminomaleimide fluorophores: a simple functional group with bright, solvent dependent emission

“…Bis-maleimides have been used to allow detection of bis-thiols (including reduced disulfides), 9 – 11 and maleimide-functional probes have been used for protein labelling, 12 and both single and two-photon imaging of cells where they allow detection of thiol functional peptides. 9 , 10 , 13 – 16 …”

Nanogels as imaging agents for modalities spanning the electromagnetic spectrum

“…Encapsulation of dyes into nanogels without covalent attachment has also been reported. 19 , 123 Cao et al encapsulated a pH-sensitive dye, 8-hydroxypyrene-1-carbaldehyde (HPC), into polyurethane nanogels as an intracellular fluorescence pH indicator. 19 The nanogels' pH sensitivity allowed imaging of H 2 O 2 -induced cytosolic acidosis.…”

Endowing a triarylboron compound showing ACQ with AIE characteristics by transforming its emissive TICT state to be dark

“…[3][4][5][6][7] Considering their molecular structure can be modied to achieve a diversity of functions, recently they have also been successfully applied to the area of bio-and chemosensing by others and by us. [8][9][10][11][12] Some triarylboron-based biological uorescence probes have been developed, including imaging for intracellular temperature, ATP, H 2 S, biothiol and mitochondria etc. Although they possess some excellent photophysical properties, including a large two-photon crosssection 8,12 and high quantum yields, and are extremely sensitive to changes in their surroundings, 6,7,9,10 we realized that in the experimental process, they usually demonstrate stronger uorescence in solution than in the solid state; they suffer from the same aggregation-caused quenching (ACQ) problem with some traditional uorophores.…”

“…[8][9][10][11][12] Some triarylboron-based biological uorescence probes have been developed, including imaging for intracellular temperature, ATP, H 2 S, biothiol and mitochondria etc. Although they possess some excellent photophysical properties, including a large two-photon crosssection 8,12 and high quantum yields, and are extremely sensitive to changes in their surroundings, 6,7,9,10 we realized that in the experimental process, they usually demonstrate stronger uorescence in solution than in the solid state; they suffer from the same aggregation-caused quenching (ACQ) problem with some traditional uorophores. 13 Therefore, to overcome this drawback and further expand their applications, it is urgent for us to develop some triarylboron uorophores showing stronger uorescence in the solid state than in solution.…”