Sensing and transduction of nutritional and chemical signals in filamentous fungi: Impact on cell development and secondary metabolites biosynthesis

Martı́n, Juan F.; Berg, M. A.; Themaat, Emiel Ver Loren van; Liras, Paloma

doi:10.1016/j.biotechadv.2019.04.014

Cited by 31 publications

(20 citation statements)

References 153 publications

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“…G-protein coupled receptors (GPCR) and oxylipins are two of the actors involved in such processes. It has been shown that regulation of G-protein signaling plays an important role in secondary metabolite production [31,186] (Table 10) and that oxylipins influence secondary metabolism at a transcriptional level [187]. GPCRs are involved in oxylipin response [30].…”

Section: Cell Signalingmentioning

confidence: 99%

Aflatoxin Biosynthesis and Genetic Regulation: A Review

Caceres

Khoury

et al. 2020

Toxins

198

177

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The study of fungal species evolved radically with the development of molecular techniques and produced new evidence to understand specific fungal mechanisms such as the production of toxic secondary metabolites. Taking advantage of these technologies to improve food safety, the molecular study of toxinogenic species can help elucidate the mechanisms underlying toxin production and enable the development of new effective strategies to control fungal toxicity. Numerous studies have been made on genes involved in aflatoxin B1 (AFB1) production, one of the most hazardous carcinogenic toxins for humans and animals. The current review presents the roles of these different genes and their possible impact on AFB1 production. We focus on the toxinogenic strains Aspergillus flavus and A. parasiticus, primary contaminants and major producers of AFB1 in crops. However, genetic reports on A. nidulans are also included because of the capacity of this fungus to produce sterigmatocystin, the penultimate stable metabolite during AFB1 production. The aim of this review is to provide a general overview of the AFB1 enzymatic biosynthesis pathway and its link with the genes belonging to the AFB1 cluster. It also aims to illustrate the role of global environmental factors on aflatoxin production and the recent data that demonstrate an interconnection between genes regulated by these environmental signals and aflatoxin biosynthetic pathway.

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Section: Cell Signalingmentioning

confidence: 99%

Aflatoxin Biosynthesis and Genetic Regulation: A Review

Caceres

Khoury

et al. 2020

Toxins

198

177

View full text Add to dashboard Cite

show abstract

“…Numbers of transporter DEGs in response to AV and MC increased, after longer induction duration compared with NC, but numbers decreased in response to GLU and WB, and hardly changed in response to HEC. Homologous analysis with known GPCR in filamentous fungi (Affeldt et al, 2014;Martín et al, 2019) detected 18 DEGs encoding GPCRs. Their expression was active under induction by WB and GLU during the early period ( Figure 6).…”

Section: Carbon Source-specific Transcriptional Patterns Of P Oxalicummentioning

confidence: 99%

“…POX00907, POX01263, and POX07722 were predicted to encode a cytochrome P450, an integral membrane protein and a bicarbonate transporter, respectively. Two candidate classic G protein-coupled receptors POX01263 and POX03930 containing seven transmembrane domains (TMDs) had a specific feature of classic GPCRs (Martín et al, 2019). However, POX03930 was annotated as a RAT1-like protein that is involved in fungal detoxification (Soustre et al, 1996).…”

Section: Identification Of Co-expression Modules Of Cellulase-and Xylmentioning

confidence: 99%

Weighted Gene Co-expression Network Analysis Identifies Critical Genes for the Production of Cellulase and Xylanase in Penicillium oxalicum

Zhao

Luo

et al. 2020

Front. Microbiol.

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Genes involved in cellular processes undergo environment-dependent co-regulation, but the co-expression patterns of fungal cellulase and xylanase-encoding genes remain unclear. Here, we identified two novel carbon sources, methylcellulose and 2-hydroxyethyl cellulose, which efficiently induced the secretion of cellulases and xylanases in Penicillium oxalicum. Comparative transcriptomic analyses identified carbon source-specific transcriptional patterns, mainly including major cellulase and xylanase-encoding genes, genes involved in glycolysis/gluconeogenesis and the tricarboxylic acid cycle, and genes encoding transcription factors, transporters and G protein-coupled receptors. Moreover, the weighted correlation network analysis of time-course transcriptomes, generated 17 highly connected modules. Module MEivory, comprising 120 members, included major cellulase and xylanase-encoding genes, genes encoding the key regulators PoxClrB and PoxXlnR, and a cellodextrin transporter POX06051/CdtC, which were tightly correlated with the filter-paper cellulase, carboxymethylcellulase and xylanase activities in P. oxalicum. An expression kinetic analysis indicated that members in MEivory were activated integrally by carbon sources, but their expressional levels were carbon source-and/or induction duration-dependent. Three uncharacterized regulatory genes in MEivory were identified, which regulate the production of cellulases and xylanases in P. oxalicum. These findings provide insights into the mechanisms associated with the synthesis and secretion of fungal cellulases and xylanases, and a guide for P. oxalicum application in biotechnology.

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“…In P. chrysogenum sensing of nutrients and signal transduction takes place by complex regulatory cascades that control expression of penicillin and other secondary metabolites [ 139 ], thus connecting primary and secondary metabolism. As an example, a metabolome study shows the relation between the penicillin production flux and central metabolism in P. chrysogenum under different perturbation conditions affecting the penicillin pathway and central metabolism.…”

Section: Metabolomicsmentioning

confidence: 99%

Omics Approaches Applied to Penicillium chrysogenum and Penicillin Production: Revealing the Secrets of Improved Productivity

Garcı́a-Estrada

Martı́n

Cueto

et al. 2020

Genes

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Penicillin biosynthesis by Penicillium chrysogenum is one of the best-characterized biological processes from the genetic, molecular, biochemical, and subcellular points of view. Several omics studies have been carried out in this filamentous fungus during the last decade, which have contributed to gathering a deep knowledge about the molecular mechanisms underlying improved productivity in industrial strains. The information provided by these studies is extremely useful for enhancing the production of penicillin or other bioactive secondary metabolites by means of Biotechnology or Synthetic Biology.

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Sensing and transduction of nutritional and chemical signals in filamentous fungi: Impact on cell development and secondary metabolites biosynthesis

Cited by 31 publications

References 153 publications

Aflatoxin Biosynthesis and Genetic Regulation: A Review

Aflatoxin Biosynthesis and Genetic Regulation: A Review

Weighted Gene Co-expression Network Analysis Identifies Critical Genes for the Production of Cellulase and Xylanase in Penicillium oxalicum

Omics Approaches Applied to Penicillium chrysogenum and Penicillin Production: Revealing the Secrets of Improved Productivity

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