Sensing and imaging of mitochondrial viscosity in living cells using a red fluorescent probe with a long lifetime

Chen, Bochao; Li, Chengde; Zhang, Jie; Kan, Jianfei; Jiang, Tiantian; Zhou, Jin; Ma, Huimin

doi:10.1039/c9cc03977e

Cited by 132 publications

(47 citation statements)

References 36 publications

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“…Although conventional dyes (e.g., mitochondria-tracker and lysosometracker probes) report the morphology of mitochondria and lysosomes 3 , they are insensitive to viscosity and therefore do not track the dynamic changes of an organelle's internal environment. Recently developed viscosity probes 22 can realize the imaging of the viscosity of a single organelle by using fluorescent probe with rotatable C sp 2 -C sp 2 bond [23][24][25] . In Coupa, there is also C 2p 2 -C sp 2 bond in its molecule skeleton, this may provide Coupa also the fluorescent viscosity sensing ability.…”

Section: Resultsmentioning

confidence: 99%

A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

et al. 2020

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Mitochondria–lysosome interactions are essential for maintaining intracellular homeostasis. Although various fluorescent probes have been developed to visualize such interactions, they remain unable to label mitochondria and lysosomes simultaneously and dynamically track their interaction. Here, we introduce a cell-permeable, biocompatible, viscosity-responsive, small organic molecular probe, Coupa, to monitor the interaction of mitochondria and lysosomes in living cells. Through a functional fluorescence conversion, Coupa can simultaneously label mitochondria with blue fluorescence and lysosomes with red fluorescence, and the correlation between the red–blue fluorescence intensity indicates the progress of mitochondria–lysosome interplay during mitophagy. Moreover, because its fluorescence is sensitive to viscosity, Coupa allowed us to precisely localize sites of mitochondria–lysosome contact and reveal increases in local viscosity on mitochondria associated with mitochondria–lysosome contact. Thus, our probe represents an attractive tool for the localization and dynamic tracking of functional mitochondria–lysosome interactions in living cells.

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Section: Resultsmentioning

confidence: 99%

A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

et al. 2020

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“…Ma and colleagues synthesized a red fluorescent probe 12 with long lifetime, achieving the visualization of mitochondrial viscosity in live cells [39] . Probe 12 was fabricated by attaching cationic hemicyanine to aniline through a rotatable C−C bond.…”

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

“…(C) Fluorescence imaging of intracellular viscosity under different conditions with probe 12 . Copyright 2019, Royal Society of Chemistry [39] . (D) Fluorescence imaging of viscosity distribution in liver slice tissues with probe 13 .…”

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

108

115

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Viscosity, as a vital microenvironment parameter, is tightly associated with multitudinous cellular processes and diseases. Recently, precise visualization of viscosity has started to arouse more and more interest. However, owing to the complicated character, it is still a huge challenge to directly observe viscosity in living systems. In this regard, mounting fluorescence probes are being increasingly fabricated to map viscosity inside live cells and small animals. In this minireview, the viscosity‐sensitive small molecular fluorescent probes used in bioimaging are comprehensively summarized, mainly focusing on the last three years. Moreover, the current challenges and opportunities for the development of viscosity‐specific fluorescent probes will be discussed.

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“…It has been found that micro-viscosity varies from 1 to 400 cP in cells. [147][148][149] Abnormalities in intracellular viscosity is associated with severe diseases, such as cancer, [150] AD, [151,152] diabetes, [153] HD and ALS, [154] Leigh's syndrome, [155] and atherosclerosis. [156] After examination of hemorheological parameters, it was found that vascular perturbations in AD patients result from hemorheological derangements, such as increased whole blood viscosity, decreased red cell deformability, increased red cell aggregation, and increased fibrinogen.…”

Section: Viscositymentioning

confidence: 99%

Fluorescent Diagnostic Probes in Neurodegenerative Diseases

et al. 2020

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Both chronic and acute neurodegenerative diseases have been associated with high morbidity and mortality, along with the death of neurons in different areas of the brain, and there are few or no effective curative therapy options for treatment of patients. [3,4] These disorders are a major cause of concern for the health and quality of life in the aging society, since age is the greatest risk factor for neurodegenerative disease. [5] The World Health Organization has predicted that in next 20 years neurodegenerative disease will become the second most common cause of mortality after cardiovascular disease. [6] Neurodegenerative processes begin long before their clinical symptoms are evident, and evolve for years slowly and irreversibly. Hence, there is an urgent need to diagnose neurodegenerative disease as early as possible and to distinguish between different neurodegenerative disorders with shared and unique symptoms to facilitate decision making regarding choice of treatment. Timely diagnosis is important for the adoption of therapeutic modalities in clinical trials prior to moderate dementia exhibition, to appraise and apply antidotes to maximally preserve the cognitive functions or to slow down the course of these disorders. The ability to analyze and identify risk factors for cognitive deficit would represent an extraordinary advancement in the field of dementia. [5] The main modern diagnostic technologies for the early examination of neurodegenerative disease pathology, neuroimaging techniques, include radioligandsbased positron emission tomography (PET), 3D single-photon emission-computed tomography (SPECT), and structural magnetic resonance imaging (MRI). Although PET and SPECT are the most used imaging techniques for neurodegenerative diseases, their application is limited by high cost, involvement of hazardous radiolabeled compounds, need for sophisticated instruments, and application of complex data acquisition and analysis protocols. The MRI technique has relatively low spatial resolution as well as intrinsically poor sensitivity to distinguish morphological differences between disease biomarkers and the surrounding tissue. Recently, fluorescence diagnosis technology has become an attractive and potential alternative to diagnose and probe the progression of neurodegenerative diseases, because of being rapid, noninvasive, sensitive, simple, real-time, low-cost, and high-resolution in nature. [7] There are several uses for Neurodegenerative diseases are debilitating disorders that feature progressive and selective loss of function or structure of anatomically or physiologically associated neuronal systems. Both chronic and acute neurodegenerative diseases are associated with high morbidity and mortality along with the death of neurons in different areas of the brain; moreover, there are few or no effective curative therapy options for treating these disorders. There is an urgent need to diagnose neurodegenerative disease as early as possible, and to distinguish between different disorders with overlap...

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Sensing and imaging of mitochondrial viscosity in living cells using a red fluorescent probe with a long lifetime

Abstract: A red mitochondria-targeting probe (Mito-V) with a long lifetime for viscosity assay has been successfully applied to monitor the viscosity changes in the courses of apoptosis, inflammation, hyperglycemia and antifungal medication.

Cited by 132 publications

References 36 publications

A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Fluorescent Diagnostic Probes in Neurodegenerative Diseases

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